A service-based approach to cryoEM facility processing pipelines at eBIC CryoEM facility processing pipelines at eBIC
Anna Horstmann, Stephen Riggs, Yuriy Chaban, Daniel K. Clare, Guilherme de Freitas, David Farmer, Andrew Howe, Kyle L. Morris, Daniel Hatton, T. Burnley
Abstract
The automation of cryoEM pipelines to aid data-quality analysis during acquisition poses a number of challenges, particularly at facilities where the expected data rates are high. The use of a modern service-based architecture deployed on Diamond Light Source’s on-premises cloud computing cluster to tackle some of these challenges is demonstrated, focusing on the provision of an early-stage processing pipeline for electron tomography that produces first-attempt reconstructed volumes within minutes of tilt-series acquisition.
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