PPubMed29 Sep 2016Cited 1×

Complete Genome Sequence of a Lytic <i> Siphoviridae </i> Bacteriophage Infecting Several Serovars of <i> Salmonella enterica </i> Genome Announcement Paradiso et al.

Rubina Paradiso, Serena Lombardi, Maria Grazia Iodice, Marita Georgia Riccardi, Massimiliano Orsini, Sergio Bolletti Censi, Giorgio Galiero, Giorgia Borriello

Abstract

'Marita Georgia Riccardi' 'Massimiliano Orsini' 'Sergio Bolletti Censi' 'Giorgio Galiero' 'Giorgia Borriello'] The bacteriophage 100268_sal2 was isolated from water buffalo feces in southern Italy, exhibiting lytic activity against several subspecies of Salmonella enterica. This bacteriophage belongs to the Siphoviridae family and has a 125,114-bp double-stranded DNA (ds-DNA) genome containing 188 coding sequences (CDSs).

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A lytic Siphoviridae phage (100268_sal2) with a 125 kb genome lacking virulence or resistance genes was sequenced, showing broad Salmonella serovar coverage and potential as a biocontrol agent.

What’s new

i.Isolated a broad‑host‑range lytic phage 100268_sal2 from water buffalo feces
ii.Generated a high‑coverage (2,034×) complete genome sequence (125,114 bp)
iii.Annotated 188 coding sequences, revealing no lysogeny, toxin, virulence or antibiotic‑resistance genes
iv.Classified the phage as a T5‑like Siphoviridae based on 90.8% identity to phage Stitch
v.Demonstrated potential prophylactic use against multiple Salmonella serovars

How they did it

The phage was isolated from water buffalo feces and its host range tested on several Salmonella serovars. DNA was purified with a QIAamp kit, sequenced on an Ion Torrent PGM (335,919 reads, avg 239 bp), assembled with SPAdes, finished with DraftDoctor, and annotated using Prokka followed by manual curation.

What they found

Genome length 125,114 bp with G+C 40.2%
Long terminal repeat of 11,238 nt
188 predicted CDSs (64 matched known phage genes, 124 hypothetical)
22 tRNA genes (2 likely pseudogenes)
No genes for lysogeny, toxin production, Salmonella virulence or antibiotic resistance
90.8% identity to phage Stitch, classifying it as a T5‑like Siphoviridae

Caveats

✕Over half of the predicted proteins are hypothetical with unknown functions
✕Functional assays (e.g., in vivo efficacy, safety) were not performed
✕Only plating efficiency data provided; detailed infection dynamics are lacking
✕Potential regulatory or ecological impacts of phage application remain unassessed

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Complete Genome Sequence of a Lytic <i> Siphoviridae </i> Bacteriophage Infecting Several Serovars of <i> Salmonella enterica </i> Genome Announcement Paradiso et al.

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