BbioRxiv8 Sep 2026
AnnoAudit: a marker-based protocol for auditing single-cell atlas annotations reveals annotation-driven artifacts in a widely used traumatic brain injury resource
Li Zhang, Haicheng Rao, Mengge Li, Xiaobo Qian + 3 more
Single-cell atlas annotations are routinely treated as ground truth but rarely validated before use. We present AnnoAudit, a marker-based audit protocol that combines four convergent checks—marker scoring, unsupervised clustering, margin-gated module scoring, and applicability-gated pretrained models—into a composite…