14 papers · ranked by Valyu relevance
Xiaofeng Dai, Li Shen
The human history has witnessed the rapid development of technologies such as high-throughput sequencing and mass spectrometry that led to the concept of “omics” and methodological advancement in systematically interrogating a cellular system. Yet, the ever-growing types of molecules and regulatory mechanisms being…
Parul Tyagi, Deeksha Singh, Shivangi Mathur, Ayushi Singh + 1 more
'Rajiv Ranjan'] Transcriptome sequencing or RNA-Sequencing is a high-resolution, sensitive and high-throughput next-generation sequencing (NGS) approach used to study non-model plants and other organisms. In other words, it is an assembly of RNA transcripts from individual or whole samples of functional and…
Nunzio D’Agostino, Wenli Li, Dapeng Wang
High-throughput transcriptomics has revolutionised the field of transcriptome research by offering a cost-effective and powerful screening tool. Standard bulk RNA sequencing (RNA-Seq) enables characterisation of the average expression profiles for individual samples and facilitates identification of the molecular…
Stephen B. Montgomery, Jonathan A. Bernstein, Matthew T. Wheeler
In the past 5 years transcriptome or RNA-sequencing (RNA-seq) has steadily emerged as a complementary assay for rare disease diagnosis and discovery. In this perspective, we summarize several recent developments and challenges in the use of RNA-seq for rare disease investigation. Using an accessible patient sample…
Tanner Stokes, Haoning Howard Cen, Philipp Kapranov, Iain J Gallagher + 7 more
'Iain J Gallagher' 'Andrew A. Pitsillides' 'Claude‐Henry Volmar' 'William E Kraus' 'James D. Johnson' 'Stuart M. Phillips' 'Claes Wahlestedt' 'James A. Timmons'] Title: Abstract Sequencing the human genome empowers translational medicine, facilitating transcriptome-wide molecular diagnosis, pathway biology, and drug…
Linlin Zhang, Dongsheng Chen, Dongli Song, Xiaoxia Liu + 3 more
'Xun Xu' 'Xiangdong Wang'] The combination of spatial transcriptomics (ST) and single cell RNA sequencing (scRNA-seq) acts as a pivotal component to bridge the pathological phenomes of human tissues with molecular alterations, defining in situ intercellular molecular communications and knowledge on spatiotemporal…
Denis Bienroth, Hieu T. Nim, Dimitar Garkov, Karsten Klein + 3 more
'Sabrina Jaeger-Honz' 'Mirana Ramialison' 'Falk Schreiber'] Spatially resolved transcriptomics is an emerging class of high-throughput technologies that enable biologists to systematically investigate the expression of genes along with spatial information. Upon data acquisition, one major hurdle is the subsequent…
Cristian D. Gutierrez Reyes, Gerardo Alejo-Jacuinde, Benjamin Perez Sanchez, Jesus Chavez Reyes + 7 more
'Benjamin Perez Sanchez' 'Jesus Chavez Reyes' 'Sherifdeen Onigbinde' 'Damir Mogut' 'Irma Hernández-Jasso' 'Denisse Calderón-Vallejo' 'J. Luis Quintanar' 'Yehia Mechref' 'Madhav Bhatia'] Traditional methodologies often fall short in addressing the complexity of biological systems. In this regard, system biology omics…
Kevin Vo, Sharmin Shila, Yashica Sharma, Grace J. Pei + 5 more
'Cinthia Y. Rosales' 'Vinesh Dahiya' 'Patrick E. Fields' 'M. A. Karim Rumi' 'Christiane Branlant'] Most eukaryotic genes express more than one mature mRNA, defined as transcript variants. This complex phenomenon arises from various mechanisms, such as using alternative transcription start sites and alternative…
Xin Zhang, Qiqi Cao, Shreya Rajachandran, Edward J Grow + 2 more
In the early days of ST, physical microdissection techniques were used to isolate molecules at specific spatial locations, such as those in LCM (), Tomo-Seq (), and spatial transcriptomics by reoriented projections and sequencing (STRP-seq) (). LCM can efficiently and accurately obtain target cell subgroups or single…
Elena A. Ponomarenko, George S. Krasnov, Olga I. Kiseleva, Polina A. Kryukova + 7 more
'Polina A. Kryukova' 'Viktoriia A. Arzumanian' 'Georgii V. Dolgalev' 'Ekaterina V. Ilgisonis' 'Andrey V. Lisitsa' 'Ekaterina V. Poverennaya' 'Jinghua Zhao' 'Jiadong Ji'] Transcriptomics methods (RNA-Seq, PCR) today are more routine and reproducible than proteomics methods, i.e., both mass spectrometry and…
Jeremy W. Prokop, Stephanie M. Bilinovich, Ember Tokarski, Sangeetha Vishweswaraiah + 27 more
The pilot human genome, completed in 2003-2004, enabled the new era of sequencing-based discoveries, with 99% of the genome mapped, enabling searching for RNA coding genes (30). The genome was narrowed to approximately 20,000-25,000 protein-coding genes based on bioinformatic filtering of open reading frames. The…
Marina Reixachs‐Solé, Eduardo Eyras
The high-throughput sequencing of cellular RNAs has underscored a broad effect of isoform diversification through alternative splicing on the transcriptome. Moreover, the differential production of transcript isoforms from gene loci has been recognized as a critical mechanism in cell differentiation, organismal…
Nico Valerio Giger, Esther Wehrle
Purpose of Review Spatial transcriptomics enables to capture the whole transcriptome within the local microenvironment in bone. Within this review, we provide an overview of recent spatial transcriptomics applications and indicate its potential for advancing basic and translational research in skeletal development and…