16 papers · ranked by Valyu relevance
Authors not listed
Genetically encoded covalent warheads at the protein-protein interface, enable the locking of interaction from the transient complex into a stable covalent adduct, which could improve the binding affinity, selectivity, and kinetics between a protein binder and its target. Given the diverse chemical microenvironments…
Irene Shajan, Lea Rochet, Shannon Tracey, Rania Benazza + 7 more
Redirecting T cells to tumor cells by bispecific antibodies is an effective approach to treat cancer and T cell-dependent bispecific antibodies (TDBAs) are an emerging class of potent immunotherapeutic agents. By simultaneously targeting antigens on tumor cells and that on T cells, T cells are activated to kill tumor…
Andrew Phillips, Anusha Srinivas, Ilina Prentoska Prentoska, Margaret O'Dea + 5 more
Teaching chemistry and chemical engineering students about biologics formulation remains challenging despite its increasing importance in pharmaceutical development. Monoclonal antibodies, commonly called mAbs, are the most popular biologics. They have been developed into drugs to treat various diseases in the past…
Yutaka Matsuda, Natsuki Shikida, Noriko Hatada, Kei Yamada + 10 more
A traceless site-selective conjugation method, “AJICAP-M,” was developed for native antibodies at specific sites using Fc-affinity peptides, focusing on Lys248 or Lys288. It produces antibody-drug conjugates (ADCs) with consistent drug-to-antibody ratios, enhanced stability, and simplified manufacturing. Comparative in…
Authors not listed
Helicobacter pylori adhesin A (HpaA) is a surface-associated protein critical for the adhesion of H. pylori to gastric epithelial cells, which is strongly associated with an increased risk of gastric cancer. Recent elucidation of the 3D structure of HpaA offers novel opportunities for targeted therapeutic strategies to…
Authors not listed
Processing high dimensional and complex monoclonal antibody (mAb) bioprocess data in industry is now more efficient due to conversational AI. The human in the loop approach to Large Language Model (LLM) inferencing with document retrieval and chained outputs is a probable benefit to existing biotechnology workflows.…
David Cate, Helen Hsieh, Veronika Glukhova, Joshua D Bishop + 15 more
The global COVID-19 pandemic has created an urgent demand for large numbers of inexpensive, accurate, rapid, point-of-care diagnostic tests. Analyte-based assays are suitably inexpensive and can be rapidly mass-produced, but for sufficiently accurate performance they require highly optimized antibodies and assay…
Liping Zhou, Leyun Wu, Cheng Peng, Yanqing Yang + 3 more
The emergence of coronavirus disease 2019 (COVID-19) pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been bringing the world to a standstill. Beyond all doubt, the most striking therapeutic target for antibody development is the spike (S) protein on the surface of virus. In contrast…
Ana Díaz-Fernández, Simona Ranallo, Francesco Ricci
Here we report the development of a method for the electrochemical ultrasensitive detection of antibodies that couples the programmability and versatility of DNA-based systems with the sensitivity provided by enzymatic amplification. The platform, termed Enzyme-Linked DNA Displacement (ELIDIS), is based on the use of…
Yuchen Bai, Jie Fei, Weilin Wu, Leina Dou + 6 more
The pursuit of the limit between dimensionalities is a scientific goal with high applicability. Sandwich immunoassay, usually based on two antibodies binding two epitopes, is one of the most popular mainstay tools in both academic and industrial fields. Herein, we determined and evaluated the minimum distance of two…
Amit Patel, Rahul Sharma
The human immune system is a complex network of cells, tissues, and organs that work together to protect the body from harmful pathogens. Antibodies, also known as immunoglobulins (Ig), are small proteins that play a vital role in the immune system's defense mechanism. Among the five classes of immunoglobulins…
Authors not listed
Antibody therapeutics can effectively target protein–protein interactions; however, translating antibody recognition motifs into short peptide ligands remains challenging due to the loss of conformational control outside the antibody framework. In particular, CDR-derived peptides often require additional constraints to…
Brad Evans, Lai Yeung, Armen Beck, Annie Li + 3 more
The growth of therapeutic monoclonal antibodies continues to accelerate due to their success as treatments for many diseases. As new therapeutics are developed, it is increasingly important to have robust bioanalytical methods to measure the pharmacokinetics of the circulating therapeutic monoclonal antibodies (mAbs)…
Authors not listed
Site-specific modifications of native-sequence proteins are technologies that underpin progresses in chemical biology, diagnostics and next-generation biotherapeutics. However, the pursuit of site-specificity has often come at the expense of scalability and usability, ultimately limiting translational potential of a…
Caoimhe Robinson, VUSLAT B. JUSKA, Alan O'Riordan
Electrochemical Impedance Spectroscopy (EIS) is a surface sensitive technique which examines the impedance of an electrochemical cell over a range of frequencies. By immobilising an antigen or antibody to the electrode surface, EIS shows potential for highly specific and sensitive immunosensor performance. Following an…
Yue Huang, Hui Yin Tan, Jiaqi Yuan, Ruipeng Mu + 8 more
What happens to macromolecules in vivo? What drives structure-activity relationship and in vivo stability for antibody-drug conjugates (ADCs)? These interrelated questions are increasingly relevant due to the re-emerging importance of ADCs as an impactful therapeutic modality and the gaps that exist in our…