9 papers · ranked by Valyu relevance
Fu Der Chen, Homeira Moradi Chameh, Mandana Movahed, Hannes Wahn + 14 more
Optogenetics has transformed neuroscience by allowing precise manipulation of neural circuits with light [1–5]. However, a central difficulty has been to deliver spatially shaped light and record deep within the brain without causing damage or significant heating. Current approaches form the light beam in free space…
Matteo Calvarese, Petra Paiè, Alessia Candeo, Gianmaria Calisesi + 7 more
Structured Illumination Microscopy (SIM) is a key technology for high resolution and super-resolution imaging of biological cells and molecules. The spread of portable and easy-to-align SIM systems requires the development of novel methods to generate a light pattern and to shift it across the field of view of the…
Mario Lachetta, Hauke Sandmeyer, Alice Sandmeyer, Jan Schulte am Esch + 2 more
Digital micromirror devices (DMDs) are spatial light modulators that employ the electro-mechanical movement of miniaturized mirrors to steer and thus modulate the light reflected of a mirror array. Their wide availability, low cost and high speed make them a popular choice both in consumer electronics such as video…
Alice Sandmeyer, Mario Lachetta, Hauke Sandmeyer, Wolfgang Hübner + 2 more
Structured illumination microscopy (SIM) is among the most widely used super-resolution fluorescence microscopy techniques for visualizing the dynamics of cellular organelles, such as mitochondria, the endoplasmic reticulum, or the cytoskeleton. In its most wide-spread implementation, SIM relies on the creation of an…
Priyash Barya, Yanyu Xiong, Skye Shepherd, Rohit Gupta + 5 more
Assays utilizing molecular fluorophores are common throughout life science research and diagnostic testing, although detection limits are generally limited by weak emission intensity, thus requiring many labeled target molecules to combine their output to achieve signal-to-noise greater than the background. Here, we…
Masakazu Iwasaka
Light projection over short distances can minimize the size of photonic devices, e.g., head-mounted displays and lens-free microscopes. Small lenses or light condensers without typical lenses are essential for light control in micron-scale spaces. In this work, micro-platelets floating in water are used for light…
Ida S. Opstad, Florian Ströhl, Marcus Fantham, Colin Hockings + 8 more
Large fields of view (FOVs) in total internal reflection fluorescence microscopy (TIRFM) via waveguides have been shown to be highly beneficial for single molecule localisation microscopy on fixed cells [1, 2] and have also been demonstrated for short-term live-imaging of robust cell types [3–5], but not yet for…
Masakazu Iwasaka
Microscopic observation system without lens has a potential to realize ubiquitous sensing network detecting micro/nano biological hazards to modern society because the lens-free imaging device can provide an extremely compact microscope. In addition to in slico micro-mirrors, liquid injectable organic micro-mirrors…
Anders Kokkvoll Engdahl, Surjendu Bikash Dutta, Stefan Belle, Jasmin Schürstedt + 5 more
Photonic waveguide chips offer near-field excitation of biological samples, which enables cost-effective, large field-of-view super-resolution microscopy without the need for high numerical aperture (NA) objective lenses. Single molecule localization based super-resolution microscopy that requires high illumination…