13 papers · ranked by Valyu relevance
Teruhiko Suzuki, Satoko Takagi, Takahiko Hara
Mouse embryonic stem cells (ESCs) are powerful tools for functional analysis of stem cell-related genes; however, complex gene manipulations, such as locus-targeted introduction of multiple genes and conditional gene knockout conditional knockout, are technically difficult. Here, we review recent advances in…
Szu-Hsien Sam Wu, Heetak Lee, Réka Szép-Bakonyi, Gabriele Colozza + 12 more
'Ayse Boese' 'Krista R. Gert' 'Natalia Hallay' 'Ji-Hyun Lee' 'Jihoon Kim' 'Yi Zhu' 'Margot M. Linssen' 'Sandra Pilat-Carotta' 'Peter Hohenstein' 'Hans-Christian Theussl' 'Andrea Pauli' 'Bon-Kyoung Koo'] The generation of conditional alleles using CRISPR technology is still challenging. Here, we introduce a Short…
Szu-Hsien Sam Wu, Somi Kim, Heetak Lee, Ji-Hyun Lee + 12 more
'Réka Bakonyi' 'Isaree Teriyapirom' 'Natalia Hallay' 'Sandra Pilat-Carotta' 'Hans-Christian Theussl' 'Jihoon Kim' 'Joo-Hyeon Lee' 'Benjamin D. Simons' 'Jong Kyoung Kim' 'Gabriele Colozza' 'Bon-Kyoung Koo'] Image-based lineage tracing enables tissue turnover kinetics and lineage potentials of different adult cell…
Hui Shi, Qin Jin, Fangbing Chen, Zhen Ouyang + 9 more
Conditional loss and restoration of function are becoming important approaches for investigating gene function. Given that reversible conditional gene knockouts in cells required complicated manipulation, conditional inactivation and reactivation of a gene in primary somatic cells with limited proliferative capacity…
Mayke A. C. ten Hoor, Margot M. Linssen, Conny M. Brouwers, Jill W. C. Claassens + 2 more
Functional validation of candidate genes in congenital anomalies of the kidneys and urinary tract (CAKUT) and other disorders is essential for translating genetic discoveries into clinical applications. Conditional knockout mouse models are indispensable for studying gene function in complex organ systems. The Short…
Nela Kelam, Petar Todorović, Patricija Bajt, Nikola Pavlović + 4 more
Background/Objectives: Congenital anomalies of the kidney and urinary tract (CAKUTs) represent the leading cause of pediatric chronic kidney disease, yet the molecular mechanisms underlying these malformations remain incompletely understood. While genetic studies have identified numerous CAKUT-associated genes…
Quanjiang Ji, Hongyuan Zhang, Yifei Zhang, Wei-Xiao Wang + 4 more
New therapeutic strategies for rapid and effective treatment of tuberculosis are highly desirable, and their development can be drastically accelerated by facile genetic manipulation methods in Mycobacterium tuberculosis. CRISPR base editors allow for rapid, robust, and programmed single base substitutions and gene…
Garima Jain, Helga Ertesvåg
Rhodococcus opacus PD630 is a biotechnologically important bacterium with metabolic capability for bioremediation, metal recovery, and storage of triacylglycerols. Genome editing by homologous recombination in R. opacus is hampered by a very low combined frequency of DNA transfer and recombination. To improve…
Duo Peng, Madhuri Vangipuram, Joan Wong, Manuel D. Leonetti
CRISPR/Cas9-mediated knock-in of DNA sequences enables precise genome engineering for research and therapeutic applications. However, designing effective guide RNAs (gRNAs) and homology-directed repair (HDR) donors remains a bottleneck. Here, we present protoSpaceJAM, an open-source algorithm to automate and optimize…
Omid Oftadeh, Vassily Hatzimanikatis
Genetic manipulation of cells to couple byproduct production and growth rate is important in bioengineering and biotechnology. In this way, we can use growth rate as a selective pressure, where the mutants with higher growth have higher production capacity. Computational methods have been proposed to find knockouts…
Ehsan Motamedian, Kristaps Berzins, Reinis Muiznieks, Egils Stalidzans
Finding the best knockout strategy for coupling biomass growth and production of a target metabolite using a metabolic model is a challenge in biotechnology. In this research, a three-step method named OptEnvelope is developed based on finding minimal active reactions for a target point in the feasible solution space…
Ian Faulkner, Cholpisit Kiattisewee, Brian Darst, Pansa Leejareon + 3 more
Genetic constructs meant for metabolic engineering in nonmodel microbes often use similar genetic parts to those familiar to E. coli work. The typical workflow is to clone these parts into plasmids in E. coli before they are transferred to the nonmodel host or its genome. In many cases, the metabolic burden of these…
Adam G. Bond, Miquel Muñoz i Ordoño, Celia M. Bisbach, Conner Craigon + 7 more
Proteolysis targeting chimera (PROTAC) degraders are typically bifunctional with one moiety of an E3 ligase ligand connected to one target protein ligand via a linker. While augmented valency has been shown with trivalent PROTACs targeting two binding sites within a given target protein, or used to recruit two…