14 papers · ranked by Valyu relevance
Thi Oanh Dao, Hyun-Eui Park, Jun Ho Lee, Kyu-Min Kim + 4 more
Genetic engineering of mycobacteria is challenging due to their hydrophobic cell wall structure and slow growth rates. Despite these obstacles, significant progress has been made to develop genetic engineering tools to study gene function and pathogenesis in these organisms. This review comprehensively explores the…
Mayke A. C. ten Hoor, Margot M. Linssen, Conny M. Brouwers, Jill W. C. Claassens + 2 more
Functional validation of candidate genes in congenital anomalies of the kidneys and urinary tract (CAKUT) and other disorders is essential for translating genetic discoveries into clinical applications. Conditional knockout mouse models are indispensable for studying gene function in complex organ systems. The Short…
Andrzej Nagalski, Kamil Koziński, Suelen Baggio, Łukasz M. Szewczyk + 15 more
Psychiatric and metabolic disorders often co-occur. While shared genetic factors and cellular dysfunctions are implicated, the underlying molecular mechanisms remain poorly understood. TCF7L2, a risk gene for type 2 diabetes and autism spectrum disorder, is highly expressed in the thalamus—a brain region extensively…
Nela Kelam, Petar Todorović, Patricija Bajt, Nikola Pavlović + 4 more
Background/Objectives: Congenital anomalies of the kidney and urinary tract (CAKUTs) represent the leading cause of pediatric chronic kidney disease, yet the molecular mechanisms underlying these malformations remain incompletely understood. While genetic studies have identified numerous CAKUT-associated genes…
Ankeeta Koirala, Kyle O’Connor, Samantha Bare, Max Toubin + 6 more
Engineering conditional alleles remains a major challenge in functional genomics. Short Artificial Introns (SAIs) have emerged as powerful tools to simplify allele design and characterization, yet practical guidelines for their implementation remain limited. Here, we describe two streamlined strategies, eSPLIT (exon…
Connor P. Lynch, Seona Thompson, Erin-Claire Pallott, Laura Campbell + 1 more
Recent research examining classical type two alarmin TSLP has uncovered non-epithelial cellular sources of the cytokine during various murine challenges. Trichuris muris is a key model of type two immunity in the intestine with expulsion being TSLP-dependent. Previous research was suggestive of epithelial production of…
Katherine G. English, Shelby N. Rorrer, Muthukumar Karuppasamy, Kathleen A. Becker + 4 more
Dedicator of cytokinesis (DOCK) proteins have diverse and critical roles in myoblast fusion, glucose metabolism, skeletal muscle regeneration, and neuronal polarity. DOCK7 is part of this 11-member family of atypical guanine exchange factors. While human DOCK7 pathogenic variants are rare, biallelic variants are known…
Yi Lin, Xiaolei Ye, Ling Zeng, Yuting Luo + 3 more
In Drosophila genetic studies, clonal analysis such as mosaic and Mosaic Analysis with a Repressible Cell Marker has been widely used to investigate gene function. Recently, the CRISPR/Cas9 system has been established as a powerful tool for efficient mutant generation; however, its application in clonal analysis has…
Alessandro Stamilla, Deborah Recchia, Giovanni Stelitano, Ludovica Maci + 5 more
Mycobacterium tuberculosis (Mtb), the etiological agent of tuberculosis, is one of the most challenging pathogens due to its complex physiology, diverse clinical manifestations, and growing multidrug resistance. The global rise of drug-resistant Mtb strains has prompted the search for innovative genetic and molecular…
Miriam Ruhinda, Roenick P. Olmo, Bianca C. Burini
Stability with Small Molecules: A Review of the ecDHFR Destabilizing Domain System Authors: Miriam Ruhinda, Roenick P. Olmo, Bianca C. Burini The E. coli dihydrofolate reductase (ecDHFR) destabilizing domain (DD) is a versatile post-translational tool for the conditional control of protein stability via ligand-induced…
Shun Zhang, Wen Sun, Ting Xiao, Yali Wang + 4 more
Controlled activation of CRISPR-Cas12a is critical for achieving conditional gene editing and molecular diagnostics. As an indispensable component for forming an active complex, CRISPR RNA (crRNA) represents a key route to regulate Cas12a activity. Here, we establish TopCas (Topology-gated Cas12a via DNA-RNA Chimeric…
Andrea Zounková, Daniele Chirivì, Adéla Přibylová, Damiano Martignago + 6 more
CRISPR-Cas9 has emerged as a powerful tool for targeted genome editing in plants; however, its application in tetraploid potato (Solanum tuberosum ssp. tuberosum) remains challenging due to its vegetative propagation and complex highly heterozygous genome. Availability of whole-genome sequence data for the specific…
Katie Willis, Austin Burt
Genetic interventions to modify wild population densities are typically framed around pest suppression, with parallel approaches for boosting beneficial or endangered populations remaining largely undeveloped. Imposing a sustained but non-eliminative genetic load could in principle address both objectives, but existing…
Ramon Diaz-Uriarte, Íñigo Ríos-Arroyo, Iain G. Johnston
Evolutionary accumulation models (EvAMs), also known as cancer progression models (CPMs), infer dependencies in the order of accumulation of mutations during tumor progression from cross-sectional data. It has been suggested that EvAMs could be used to identify therapeutic targets, but there is no procedure in the…