14 papers · ranked by Valyu relevance
Senik Matinyan, Jan Pieter Abrahams
High-throughput data collection in crystallography poses significant challenges in handling massive amounts of data. Here, we present TERSE, a novel lossless compression algorithm specifically designed for diffraction data. We compare TERSE with the established lossless compression algorithms implemented in gzip, CBF…
Steve P. Meisburger, Nozomi Ando
Diffuse scattering is a powerful technique to study disorder and dynamics of macromolecules at atomic resolution. Although diffuse scattering is always present in diffraction images from macromolecular crystals, the signal is weak compared with Bragg peaks and background, making it a challenge to visualize and measure…
Max T.B. Clabbers, Johan Hattne, Michael W. Martynowycz, Tamir Gonen
A favorable signal-to-noise ratio is essential for obtaining high-quality diffraction data in macromolecular electron crystallography. Inelastic scattering contributes significantly to the noise, reducing contrast between diffraction peaks and background, which complicates peak detection and compromises the accuracy of…
Thomas A. Hensel, Jan O. Wirth, Stefan W. Hell
Discerning two or more identical and constantly scattering point sources using freely propagating waves is thought to be limited by diffraction. Here we show both theoretically and experimentally that by employing a diffraction minimum rather than a maximum for resolution, a given number of point scatterers can be…
Marcus Gallagher-Jones, Robert Bücker, Quentin Delaunay, Nicolas Coquelle + 8 more
Bacillus thuringiensis is one of the most widely used biopesticides worldwide owing to the highly specific pesticidal-proteins various strains produce in the form of nanocrystals. Structure determination of such crystals remains difficult because their small size makes them unsuitable for conventional X-ray…
Johan Hattne, Max T. B. Clabbers, Michael W. Martynowycz, Tamir Gonen
The combination of high sensitivity and rapid readout makes it possible for electron-counting detectors to record cryogenic electron microscopy data faster and more accurately without increasing the exposure. This is especially useful for MicroED of macromolecular crystals where the strength of the diffracted signal at…
Che-Hang Yu, Yiyi Yu, Joseph S. Canzano, Yuandong Fei + 1 more
In many laser scan engines, mechanical inertia imposes a tradeoff between mirror diameter (mass) and scan speed (cycle time) and/or scan angle amplitude. Ultimately, this limits the field-of-view and/or scan speed in multiphoton and confocal imaging, laser-based manufacturing, and other applications. To push parameters…
Stephane Bancelin, Johannes Roos, U. Valentin Nägerl
We introduce Faser, a software package developed in Python as a plugin for the open-source napari platform, designed to simulate the excitation point spread functions (PSFs) of microscopes. Using a full-vectorial computational approach to simulate the electromagnetic fields within the focal region, it makes precise…
Shervin S. Nia, Ambarneil Saha, Jung Cho, Z. Hong Zhou + 2 more
Macromolecular crystallography has historically inferred models of internal crystal architecture from reciprocal-space measurements of Bragg reflections. Nevertheless, direct real-space visualization of crystallographic disorder remains elusive, particularly at the nanoscale. Using a 15-nanometer probe, here we apply…
Maximilian K. Geismann, Alba Gomez-Segalas, Alessandro Passera, Mehrta Shirzadian + 1 more
Displacing beams of light in a controlled manner is integral to a wide range of optical methods such as scanning microscopes and optical tweezers. Optical super-resolution techniques like STED and RESOLFT, as well as localization techniques like MINFLUX and MINSTED rely on accurate and fast beam displacements.…
Cathie Ventalon, Agathe Nidriche, Delphine Débarre
Sectioning techniques based on patterned illumination have been widely used to obtain well-contrasted images of thick samples using widefield imaging setups. While their application to fluorescence microscopy has been extensively demonstrated and studied, their application to reflection imaging is scarcer and their…
Chetna Taneja, Jerin Geogy George, Stella Corsetti, Philip Wijesinge + 4 more
Bessel beams (BB) have found widespread adoption in various forms of light-sheet microscopy. However, for one-photon fluorescence, the transverse profile of the beam poses challenges due to the detrimental effect of the sidelobes. Here, we mitigate this issue by using a computer generated phase element for generating a…
Anna Archetti, Matteo Bruzzone, Giulia Tagliabue, Marco dal Maschio
Bessel Beams (BBs) and BB lattices are structured-light excitation profiles frequently applied in material processing, nonlinear spectroscopy and in many fluorescence microscopy methods such as Light Sheet Microscopy (LSM). In LSM, BBs and BB-lattices offer wider excitation profiles, higher acquisition rate, enhanced…
Thomas D. Schneider
Unlike the Carnot heat engine efficiency published in 1824, an isothermal efficiency derived from thermodynamics and information theory can be applied to biological systems. The original approach by Pierce and Cutler in 1959 to derive the isothermal efficiency equation came from Shannon’s channel capacity of 1949 and…