16 papers · ranked by Valyu relevance
Johan Hattne, Michael W. Martynowycz, Tamir Gonen
Microcrystal electron diffraction (MicroED) combines crystallography and electron cryomicroscopy (cryo-EM) into a method that can be used for high-resolution structure determination. In MicroED nanosized crystals, often intractable by other techniques, are probed by high-energy electrons in a transmission electron…
Max T.B. Clabbers, Michael W. Martynowycz, Johan Hattne, Brent L. Nannenga + 1 more
Microcrystal electron diffraction (MicroED) uses electron cryo-microscopy (cryo-EM) to collect diffraction data from small crystals during continuous rotation of the sample. As a result of advances in hardware as well as methods development, the data quality has continuously improved over the past decade, to the point…
Heng zhou, Feng Luo, Zhipu Luo, Dan Li + 2 more
Microcrystal electron diffraction (MicroED) is becoming a powerful tool in determining the crystal structures of biological macromolecules and small organic compounds. However, wide applications of this technique are still limited by the special requirement for radiation-tolerated movie-mode camera and the lacking of…
Johan Hattne, Max T. B. Clabbers, Michael W. Martynowycz, Tamir Gonen
The combination of high sensitivity and rapid readout makes it possible for electron-counting detectors to record cryogenic electron microscopy data faster and more accurately without increasing the exposure. This is especially useful for MicroED of macromolecular crystals where the strength of the diffracted signal at…
Emma V. Beale, David G. Waterman, Corey Hecksel, Jason van Rooyen + 6 more
Micro-Electron Diffraction (MicroED) has recently emerged as a powerful method for the analysis of biological structures at atomic resolution. This technique has been largely limited to protein nanocrystals which grow either as needles or plates measuring only a few hundred nanometres in thickness. Furthermore…
Gerhard Hofer, Lei Wang, Laura Pacoste, Paul Hager + 9 more
Determining macromolecular structures is crucial for understanding biological mechanisms and advancing drug discovery. Three-dimensional electron diffraction (3D ED), also known as microcrystal electron diffraction (MicroED) using continuous sample rotation has emerged as a powerful method for solving structures from…
Koji Yonekura, Tetsuya Ishikawa, Saori Maki-Yonekura
A new cryo-EM system has been developed and investigated for use in protein electron 3D crystallography. The system provides parallel illumination of a coherent 300 kV electron beam to a sample, filters out energy-loss electrons through the sample with an in-column energy filter, and allows rotational data collection…
Kiyofumi Takaba, Saori Maki-Yonekura, Koji Yonekura
A semi-automated protocol has been developed for rotational data collection of electron diffraction patterns by combined use of SerialEM and ParallEM, where SerialEM is used for positioning of sample crystals and ParallEM for rotational data collection. ParallEM calls standard camera control software through an AutoIt…
Marcus Gallagher-Jones, Karen C. Bustillo, Colin Ophus, Logan S. Richards + 4 more
Nanocrystallography has transformed our ability to interrogate the atomic structures of proteins, peptides, organic molecules and materials. By probing atomic level details in ordered sub-10 nm regions of nanocrystals, approaches in scanning nanobeam electron diffraction extend the reach of nanocrystallography and…
Thorsten B. Blum, Dominique Housset, Max T.B. Clabbers, Eric van Genderen + 6 more
Electron diffraction allows protein structure determination when only nanosized crystals are available. Nevertheless, multiple elastic (or dynamical) scattering, prominent in electron diffraction, is a concern. Current methods for modeling dynamical scattering by multi-slice or Bloch wave approaches are not suitable…
Michael W. Martynowycz, Max T.B. Clabbers, Johan Hattne, Tamir Gonen
Structures of two globular proteins were determined ab initio using microcrystal electron diffraction (MicroED) data that was collected on a direct electron detector in counting mode. Microcrystals were identified using a scanning electron microscope (SEM) and thinned with a focused ion-beam (FIB) to produce…
Vitaly Polovinkin, Krishna Khakurel, Michal Babiak, Borislav Angelov + 4 more
Electron crystallography of sub-micron sized 3D protein crystals has emerged recently as a valuable field of structural biology. In meso crystallization methods, utilizing lipidic mesophases, particularly lipidic cubic phases (LCPs), can produce high-quality 3D crystals of membrane proteins (MPs). A major step towards…
Johan Hattne, Dan Shi, Calina Glynn, Chih-Te Zee + 4 more
Micro-crystal electron diffraction (MicroED) is an emerging method in cryo-EM for structure determination using nanocrystals. It has been used to solve structures of a diverse set of biomolecules and materials, in some cases to sub-atomic resolution. However, little is known about the damaging effects of the electron…
Necati Atalay, Enver Kamil Akcan, Mehmet Gül, Esra Ayan + 43 more
X-ray crystallography is a robust and powerful structural biology technique that provides high-resolution atomic structures of biomacromolecules. Scientists use this technique to unravel mechanistic and structural details of biological macromolecules (e.g. proteins, nucleic acids, protein complexes, protein-nucleic…
Ruizhi Peng, Xiaofeng Fu, Joshua H. Mendez, Peter S. Randolph + 2 more
Advances in electron detection have been essential to the success of high-resolution cryo-EM structure determination. A new generation of direct electron detector called the Apollo, has been developed by Direct Electron. The Apollo uses a novel event-based MAPS detector custom designed for ultra-fast electron counting.…
Mads Bertelsen, Peter K. Willendrup, Sunyoung Yoo, Adriano Meligrana + 4 more
Monte Carlo neutron ray-tracing simulations of time-of-flight (TOF)-Laue neutron macromolecular crystal diffraction (n-MX) using the McStas software package were done for the upcoming NMX Macromolecular Diffractometer at the European Spallation Source. Splitting neutron rays that arrive at the crystal lead to dramatic…