21 papers · ranked by Valyu relevance
Yanying Yu, Sandra Gawlitt, Lisa Barros de Andrade e Sousa, Erinc Merdivan + 3 more
CRISPR interference (CRISPRi), the targeting of a catalytically dead Cas protein to block transcription, is the leading technique to silence gene expression in bacteria. Genome-scale CRISPRi essentiality screens provide one data source from which rules for guide design can be extracted. However, depletion confounds…
Longyu Wang, Xiaochen Xie, Yang Liu, Wenqiang Li + 8 more
Argonaute proteins are widespread in prokaryotes and eukaryotes. Most prokaryotic Argonaute proteins (pAgos) use 5’P-gDNA to target complementary DNA. However, more and more studies on the properties of pAgos make their functions more diversified. Previously reported pAgos only possess several forms of high activity in…
Yanying Yu, Sandra Gawlitt, Lisa Barros de Andrade e Sousa, Erinc Merdivan + 3 more
'Erinc Merdivan' 'Marie Piraud' 'Chase L. Beisel' 'Lars Barquist'] CRISPR interference (CRISPRi) is the leading technique to silence gene expression in bacteria; however, design rules remain poorly defined. We develop a best-in-class prediction algorithm for guide silencing efficiency by systematically investigating…
Y K Yathu Krishna
The efficiency and specificity of guide RNAs continue to be crucial obstacles for successful experimental design, despite the fact that CRISPR/Cas9 has transformed genome editing. In this work, we introduce a computational method for optimizing CRISPR/Cas9 guide RNA that combines PAM diversity, local efficiency…
Liang Zhang, Wei He, Rongjie Fu, Han Xu
High-fidelity Cas9 variants have been developed to reduce the off-target effects of CRISPR systems at a cost of efficiency loss. To systematically evaluate the efficiency and off-target tolerance of Cas9 variants in complex with different single guide RNAs (sgRNAs), we applied high-throughput viability screens and a…
Thomas Burgold, Emre Karakoc, Emanuel Gonçalves, Lisa Dwane + 9 more
Pairwise perturbation of gene function using the CRISPR/Cas9 system has huge potential in screening for genetic interactions and synthetic lethal gene pairs to identify novel combination therapies for cancer. However, existing dual guide expression systems are cumbersome to clone, often result in a large proportion of…
Qian Xiao, Jiayang Li
Current emergency management research mainly specifies the positions of evacuation guides from a knowledge base of experience, disregarding the subjective perceived decision-making of pedestrians caught in an emergency situation. Therefore, in this paper, a fuzzy inference system for pedestrians to select guides is…
Aisha Ali, Ido Brintouch, Georgios Romanos, Rafael Delgado-Ruiz + 1 more
'Edgaras Stankevičius'] Background and Objectives: Surgical guides might impede the flow of coolant to the implant drills during the preparation of the implant bed, potentially contributing to increased temperatures during bone drilling. The objective of this experimental study was to assess the cooling efficiency of…
Gregory R. Roytman, Alim F. Ramji, Brian Beitler, Brad Yoo + 4 more
'Michael P. Leslie' 'Michael Baumgaertner' 'Steven Tommasini' 'Daniel H. Wiznia'] Background The goal of stabilization of the femoral neck is to limit morbidity and mortality from fracture. Of three potential methods of fixation, (three percutaneous screws, the Synthes Femoral Neck System, and a dynamic hip screw)…
Shomik Verma, Daniel Farrell, Rachel Evans
Luminescent solar concentrators (LSCs) are a promising technology to help integrate solar cells into the built environment, as they are colorful, semi-transparent, and can collect diffuse light. While LSCs have traditionally been cuboidal, in recent years a variety of unconventional geometries have arisen, for example…
Margaréta Halická, Mária Trnovská, Aleš Černý
The paper analyses properties of a large class of "path-based" Data Envelopment Analysis models through a unifying general scheme. The scheme includes the well-known oriented radial models, the hyperbolic distance function model, the directional distance function models, and even permits their generalisations. The…
Adam Nulty
This is an extensive review of the literature on prosthetically designed implant planning with particular regard to the factors influencing dental implant success. Electronic searches on PubMed and the Cochrane Central Register of Controlled Trials and manual searches were performed. The author selected the studies…
Dean F Sittig, Trisha Flanagan, Patricia Sengstack, Rosann T Cholankeril + 6 more
Each of the 2014 SAFER Guides was envisioned as a stand-alone Guide that could be used by different groups within a healthcare organization to address known or potential EHR-related safety issues within the particular domain addressed by the Guide. Following CMS’s requirements that all hospitals must review all Guides…
Fan Yang, Pengcheng Chang, Yiping Cai, Zhikang Lin + 3 more
'Yuting Lv' 'Jean-Noël Jaubert'] The energy loss of the vertical axial flow pump device increases due to the unstable internal flow, which reduces the efficiency of the pump device and increases its energy consumption of the pump device. The research results of the flow loss characteristics of the total internal…
Silvia Izabella Pop, Eugen Bud, Kinga Mária Jánosi, Anamaria Bud + 2 more
'Bernadette Kerekes-Máthé' 'Luca Levrini'] Surgical guides are integral tools in orthodontics, enhancing the precision and predictability of mini-implant placement. These guides facilitate accurate positioning, reduce risks to surrounding anatomical structures, and ensure proper angulation and depth during procedures.…
Diogo Cunha Ferreira, José Rui Figueira, Salvatore Greco, Rui Cunha Marques
'Rui Cunha Marques'] Assessing the technical efficiency of a set of observations requires that the associated data composed of inputs and outputs are perfectly known. If this is not the case, then biased estimates will likely be obtained. Data Envelopment Analysis (DEA) is one of the most extensively used mathematical…
Zhimian Hao, Chonghuan Zhang, Alexei Lapkin
We propose a workflow for reduction in the time required for data generation during generation of statistical digital twins. This methodology is particularly relevant for real-world engineering problems when data generation is expensive. A prerequisite for building surrogates is sufficient input/output data, whereas…
Thomas Standfuß, Georg Hirte, Michael Schultz, Hartmut Fricke
We systematically study cornerstones that must be solved to define an air traffic control benchmarking system based on a Data Envelopment Analysis. Primarily, we examine the appropriate decision-making units, what to consider and what to avoid when choosing inputs and outputs in the case that several countries are…
Ami Marowka
Portability Authors: ['Ami Marowka'] Abstract. The emergence of heterogeneity in high-performance computing, which harnesses under one integrated system several platforms of different architectures, also led to the development of innovative crossplatform programming models. Along with the expectation that these models…
Fulai Yao
Devices Authors: ['Fulai Yao'] Abstract- The energy efficiency optimization of the power generation system and the energy efficiency optimization of the energy consumption system are unified into the same optimization problem, and a simple method to achieve energy efficiency optimization without establishing an…
Brianna Greenstein, Geoffrey Hutchison
Tandem organic solar cells can potentially drastically improve the PCE over single-junction devices. However, there is limited research on device development and often only ca. 1% improvement over single-junction devices. Because of the complex nature of organic material compatibility and properties, such as…