22 papers · ranked by Valyu relevance
R. Balamurugan
Microarray gene expression data are high-dimensional and complex, with patterns that may appear only under specific conditions. Traditional clustering often misses these local patterns, whereas biclustering can reveal groups of genes with coordinated expression across particular conditions. In this paper, we propose a…
Tyrese Boddie, Arianna Lacen, Hui-Ting Lee
Methods: From RT-qPCR to Advanced Microscopy Authors: Tyrese Boddie, Arianna Lacen, Hui-Ting Lee RNA plays a crucial role in gene expression, regulation, protein synthesis, and other cellular functions. The diversity that exists between different RNAs makes information beyond their expression level necessary for…
Subhrajyoty Roy, Abhik Ghosh, Ayanendranath Basu
Estimating the true rank of a noisy data matrix is a fundamental problem underlying techniques such as principal component analysis, matrix completion, etc. Existing rank estimation criteria, including information-based and cross-validation methods, are either highly sensitive to outliers or computationally demanding…
Santra Santhosh, Sharon Istvánffy, Omer Sabary, Eitan Yaakobi + 3 more
Large-scale de novo nucleic acid synthesis is a powerful tool enabling researchers to better understand and engineer biological systems. Fields ranging from genomics to nucleic acid therapeutics to synthetic biology make use of high-throughput experimental approaches requiring access to large pools or libraries of DNA…
Igor Ilić, Erika Schaudy, Jory Lietard
The phosphorothioate bond (PS) is one of the most common chemical modifications of DNA and RNA and replaces one of the non-bridging oxygen atoms of the phosphodiester (PO) bond with a sulfur atom. Ubiquitous in oligonucleotide drug design, as it equips most FDA-approved therapeutics, it imparts resistance against…
Erika Schaudy, Jory Lietard
Chemically modifying the backbone, sugar, or nucleobase moieties of nucleic acids greatly expands their functional repertoire. Nucleobase modifications have received particular attention for their proven ability to generate a greater diversity of intra- and intermolecular interactions. This broader interaction…
Claudius L. Dietsche, Lucien R. Stöcklin, Robert Strutt, Petra S. Dittrich
Arrays for High-Throughput Quantitative Analysis of Cytokines from Single Macrophages Authors: Claudius L. Dietsche, Lucien R. Stöcklin, Robert Strutt, Petra S. Dittrich Macrophages play a critical role in the development of the tumor microenvironment (TME). Recruited macrophages in the TME differentiate into various…
Neeraja M. Krishnan, Sarah I Rahman, Lars Røn Olsen, Binay Panda
Many biological studies could benefit from combining data from legacy microarray and high-throughput sequencing platforms, especially in clinical domains where collecting additional samples is not possible. However, incompatibility between platforms makes legacy data difficult to integrate, owing to differences in…
Mikhail Sokolov, Irina S. Kriukova, Alyona Sukhanova, Igor Nabiev
Suspension microarrays based on optically encoded microbeads have become one of the most versatile platforms for multiplexed bioanalysis because they combine solution-phase reaction kinetics, flexible assay design, and high-throughput detection. However, despite more than two decades of intense research, no consensus…
Seongmin Lee, Won Il Heo, Kui Young Park, Seong Jun Seo + 3 more
Rapid and reliable on-site identification of body fluids is essential in forensic and field diagnostic applications. Commercial kits provide only single results and often suffer from cross-reactivity, while conventional microarrays offer multiplex capability but lack sufficient fluorescence intensity for…
Georgina Flórez-Grau, Till S. M. Mathan, Mihaela B. Mihaylova, Tom van Oorschot + 6 more
Ex-vivo stimulation of dendritic cells (DCs) is a critical step in DC-based cancer immunotherapies. In humans, conventional type 1 dendritic cells (cDC1s) are a rare myeloid dendritic cell (mDC) subset that express BDCA-3 (CD141). cDC1s promote CD8^+^ T cell cross-priming against tumor antigens and are therefore being…
Jarno Koetsier, Ozan Cinar, Egon L. Willighagen, Ammar Ammar + 7 more
Transcriptomic profiling has become a cornerstone of modern biomedical research. To make transcriptomic analyses accessible to a broader scientific community, specifically including researchers with limited bioinformatics expertise, we introduced ArrayAnalysis in 2013 as a user-friendly web-based application for…
Authors not listed
The capacity to pattern biomolecules within microfluidic devices expands the scope of microfluidic technologies. In such patterned systems, surface-bound components remained localized, while the microfluidic network supplies reagents and removes waste products. This approach has enabled continuous protein expression…
Li Peng, Weisi Lai, Jian Huang, Yan Zhong
Early detection of preeclampsia with noninvasive and reliable biomarkers is the foremost step for minimizing adverse effects during pregnancy. However, none maternal serum analyte evaluated so far is sufficiently accurate to allow recommending their routine use. Microarray was used to first identify differentially…
Authors not listed
Understanding and controlling the microstructure of zinc (Zn) metal electrodeposits is critical for advancing the next generation of rechargeable Zn batteries. In this study, we develop microelectrode arrays to systematically investigate the relationship between current density, morphology, and Coulombic efficiency…
Catarina Coutinho, Nuria Lafuente-Gómez, Irene de la Iglesia, Luis A. Campos + 6 more
Early, accurate, and fast diagnosis is essential to ensure positive health outcomes, through effective treatment interventions and disease control, as well as the study of physiological changes related to gene expression. Liquid biopsies and point-of-care (PoC) detection are valuable tools to achieve this goal…
Ishan Rana, Suvrath Mahadevan, Megan Delamer, Ceiwynn Longworth
Two-photon polymerization (2PP) enables the fabrication of high-precision micro-optics with complex freeform geometries, opening up a new parameter space for custom astronomical optics. Among the available resins, the newly developed IPX Clear is particularly well suited for visible applications, offering very high…
Authors not listed
Polymer-induced liquid precursors are hydrated, metastable, amorphous droplets rich in insoluble ionic compounds; they form in aqueous solutions that are supersaturated with mineral ions in the presence of charged polymers that delay the onset of crystal nucleation. This work reports that rapid crystallization of a…
Authors not listed
Understanding how plants respond to dynamic and spatially variable stimuli is a key goal in plant sciences. Traditional imaging methods often involve a trade-off between environmental control and spatial resolution, limiting their ability to capture real-time responses in high resolution. Microfluidic technology…
Agostino Occhicone, Alberto Sinibaldi, Peter Munzert, Jordan N. Butt + 4 more
This study presents a rigorous comparative analysis of two label-free optical biosensing platforms, Bloch surface wave (BSW) and microring resonator (MRR), for the detection of SARS-CoV-2 antibodies in human serum. To ensure direct comparability, a new BSW readout system was established alongside an existing MRR…
Samrat Basak, Kim Chi-Vu, Nikolaos Mougios, Nazar Oleksiievets + 6 more
DNA-based Point Accumulation for Imaging in Nanoscale Topography (DNA-PAINT) is a powerful variant of single-molecule localization microscopy (SMLM) that overcomes the limitations of photobleaching, offers flexible fluorophore selection, and enables fine control of imaging parameters through tunable on- and off-binding…
Amritha Janardanan, Soner Sonmezoglu, Stefano Sonedda, Tom J. Zajdel + 5 more
Bacteria sense a diverse range of environmental analytes with high sensitivity and temporal resolution. Engineering and synthetic biology approaches enabled harnessing this capability through development of whole-cell biosensors that respond to specific molecules of interest. However, converting these responses into…