11 papers · ranked by Valyu relevance
Li Peng, Weisi Lai, Jian Huang, Yan Zhong
Early detection of preeclampsia with noninvasive and reliable biomarkers is the foremost step for minimizing adverse effects during pregnancy. However, none maternal serum analyte evaluated so far is sufficiently accurate to allow recommending their routine use. Microarray was used to first identify differentially…
Ye Gao, Ni Wang, Zhaoyang Xiao, Alessandro Medoro
The GEOquery package (v2.74.0) was employed to retrieve datasets related to ARDS from the Gene Expression Omnibus (GEO) database ([https://www.ncbi.nlm.nih.gov/geo/]()). Specifically, the training set [GSE32707]() comprised blood samples from 58 septic patients (control group) and 31 patients with sepsis-induced ARDS…
Meili Xi, Rongkui Luo, Jiarong Zhang, Weihong Huang + 3 more
Polycystic ovary syndrome (PCOS) is a heterogeneous endocrine-metabolic disorder characterized by profound disturbances in energy metabolism, yet the molecular mechanisms underlying glycolytic dysfunction remain incompletely understood. Given the essential role of glycolysis in ovarian function and endocrine…
Yi Peng, Suobing Qin, Xiwen Li, Lijun Wan + 1 more
2.1### Data Collection and Data Preprocessing The data for this study was sourced from public databases: ccRCC gene expression profiles and clinical data were obtained from the Cancer Genome Atlas (TCGA; [https://portal.gdc.cancer.gov/](), Version: October 2023) and Gene Expression Integrated Database (GEO…
Huihai Wu, Ashleigh Lister, Iain Macaulay, Katie Long + 10 more
Single-cell and spatial transcriptomics are transforming our understanding of cellular heterogeneity and tissue organization, yet their analytical complexity remains a major bottleneck. Here, we present EISCA and EISTA, two standardized, end-to-end pipelines for single-cell RNA- seq and imaging-based spatial…
Ajay Kumar Danga, Alessio Colantoni, Nicola Pomella, Nerea Ruiz Blanes + 5 more
Autism spectrum disorder (ASD) shows a strong male bias (∼4:1). CHD8, a frequently mutated ASD gene, regulates neuronal development and Xist regulation in X-chromosome inactivation (XCI). However, its role in female neurodevelopment remains poorly understood due to the predominance of male-derived or sex-agnostic…
Wenbin Li, Rui Fan, Kun Xie, Jiehua Zhong + 12 more
EV-miRNAs are promising gastric cancer (GC) biomarkers for early diagnosis, yet their clinical application is limited by major detection challenges arising from the low abundance and high sequence homology of EV-miRNAs. Herein, we developed a dual-mode platform for sensitive EV-miRNA in situ profiling based on…
Marie‐Christin Schulz, Virginie Dubourg, Lea Schaude, Natalie Schröder + 5 more
4.2.1#### Cell Culture for RNA Sequencing and Western Blot Analysis Epithelial cells, such as HK-2 cells, attach to the substrate via their basolateral membrane, whereas the apical membrane faces toward the tubular lumen. Thus, in vivo, tubular epithelial cells are separated from the interstitial space that harbors…
Atif Khan, Sruti Chandramouli, Hiren M. Joshi
The crystal violet (CV) microtiter plate assay is widely used to compare microbial biofilm formation because it is inexpensive, simple, and readily adapted to 96-well formats. Its high-throughput design enables multiple strains, growth conditions, and treatments to be tested in parallel, but increasing experimental…
Alejandro Espinoza, Bruno J. de Andrade Silva, Matteo Pellegrini
Single-cell RNA sequencing technologies provide insights into gene expression at the cellular level, enabling detailed analysis of cellular heterogeneity. In this study, we systematically compared two scRNA-seq platforms,10X Genomics and Parse Biosciences, using human peripheral blood mononuclear cells (PBMCs) and…
Aleksandra Stanojevic, Rafael Stroggilos, Mladen Marinkovic, Ana Djuric + 7 more
Neoadjuvant chemoradiotherapy followed by total mesorectal excision is standard for locally advanced rectal cancer, but response varies and current markers are insufficient. This study integrates public bulk RNA-seq data to identify predictive features of response. TRIM54 and PABPC4 were upregulated in the responder…