11 papers · ranked by Valyu relevance
Orestis Faklaris, Leslie Bancel-Vallée, Aurélien Dauphin, Baptiste Monterroso + 9 more
Reliable, reproducible and comparable results are what biology requires from microscopy. To achieve that level of confidence, monitoring the stability of the microscope performance over time with standardized quality testing routines is essential for mining quantitative data. Three levels of microscope quality control…
Kristine A. Tsantilas, Gennifer E. Merrihew, Julia E. Robbins, Richard S. Johnson + 13 more
A thorough evaluation of the quality, reproducibility, and variability of bottom-up proteomics data is necessary at every stage of a workflow from planning to analysis. We share vignettes applying adaptable quality control (QC) measures to assess sample preparation, system function, and quantitative analysis. System…
Michael Totty, Stephanie C. Hicks, Boyi Guo
Quality control (QC) is a crucial step to ensure the reliability and accuracy of the data obtained from RNA sequencing experiments, including spatially-resolved transcriptomics (SRT). Existing QC approaches for SRT that have been adopted from single-nucleus RNA sequencing (snRNA-seq) methods are confounded by spatial…
Anisha Keshavan, Esha Datta, Ian McDonough, Christopher R. Madan + 2 more
Tissue classification plays a crucial role in the investigation of normal neural development, brain-behavior relationships, and the disease mechanisms of many psychiatric and neurological illnesses. Ensuring the accuracy of tissue classification is important for quality research and, in particular, the translation of…
Marthe Colotte, Aurélie Luis, Jacques Bonnet, Delphine Coudy + 10 more
The Coronavirus pandemic unveiled the unprecedented need for diagnostic tests to rapidly detect the presence of pathogens in the population. Real-time RT-PCR and other nucleic acid amplification techniques are accurate and sensitive molecular techniques that necessitate positive controls. To meet this need, Twist…
Omer Sabary, Yoav Orlev, Roy Shafir, Leon Anavy + 2 more
Recent years have seen a growing number and a broadening scope of studies using synthetic oligo libraries for a range of applications in synthetic biology. As experiments are growing by numbers and complexity, analysis tools can facilitate quality control and help in assessment and inference. We present a novel…
Adam P. Rosebrock
Detecting RNA viruses such as SARS-nCoV2 requires careful handling of inherently labile RNA. Molecular tests incorporate controls to verify the presence of intact RNA and ensure that all steps of an assay have succeeded. The Centers for Disease Control and Prevention (CDC)-specified extraction control recognizes both…
Cheryl A. Keller, Elisabeth F. Heuston, Belinda Giardine, Maria R. Long + 5 more
Chromatin immunoprecipitation followed by massively parallel, high throughput sequencing (ChIP-seq) is the method of choice for identifying, on a genome-wide scale, the segments of DNA bound by specific transcription factors (TFs) or in chromatin with particular histone modifications. However, the quality of ChIP-seq…
Kerstin Wernike, Markus Keller, Franz J. Conraths, Thomas C. Mettenleiter + 2 more
To combat the COVID-19 pandemic, millions of PCR tests are performed worldwide. Any deviation of the diagnostic sensitivity and specificity will reduce the predictive values of the test. Here, we report the occurrence of contaminations of commercial primers/probe sets with the SARS-CoV-2 target sequence of the RT-qPCR…
Sasithon Temisak, Pattanapong Thangsunan, Jiranun Boonil, Watiporn Yenchum + 4 more
The problem in meat adulteration and food fraud emphasised the requirement of developing accurate analytical approaches for the quantitative detection in helping the control of meat adulteration. In this study, the droplet digital Polymerase Chain Reaction (ddPCR) assays to quantify the ratios of pork DNA to the total…
Joshua Pearson, Kirill Sechkar, Harrison Steel
Bacteria and their viruses, bacteriophages (phages), continually coevolve in nature. In contrast, laboratory-based coevolution experiments usually last less than a month before becoming dormant or extinct as one species is outcompeted by the other. Consequently, there is a poor understanding of phage-bacteria…