18 papers · ranked by Valyu relevance
Peter Horvath, Fabian Coscia
Spatial proteomics offers unprecedented insights into the localization, quantity, and interactions of proteins within cells and tissues, thereby enabling researchers and clinicians to map protein networks with high precision. This powerful approach is currently revolutionizing our understanding of cellular organization…
Yiwen Li, Yusheng Zhang, Ying Zhang, Qing Wang + 3 more
Protein function is inherently spatial: the same molecule can elicit distinct biological outcomes depending on its localization, interacting partners, and surrounding microenvironment. Spatial proteomics enables systematic in situ characterization of protein localization, abundance, and interactions across subcellular…
Yuanwei Xu, T. Mamie Lih, Angelo M. De Marzo, Qing Kay Li + 1 more
Background Spatial proteomics seeks to understand the spatial organization of proteins in tissues or at different subcellular localization in their native environment. However, capturing the spatial organization of proteins is challenging. Here, we present an innovative approach termed Spatial Proteomics through…
Oliver M. Crook, Claire M. Mulvey, Paul D. W. Kirk, Kathryn S. Lilley + 2 more
Analysis of the spatial sub-cellular distribution of proteins is of vital importance to fully understand context specific protein function. Some proteins can be found with a single location within a cell, but up to half of proteins may reside in multiple locations, can dynamically re-localise, or reside within an…
Yida Wang, Yang Wu, Feng Zhang, Parthiban Periasamy + 10 more
Background: Spatial proteogenomics marks a paradigm shift in oncology by integrating molecular analysis with spatial information from both spatial proteomics and other data modalities (e.g., spatial transcriptomics), thereby unveiling tumor heterogeneity and dynamic changes in the microenvironment. Methods: We…
Ying Jiang, Jian Wang, Aihua Sun, Hongxing Zhang + 18 more
'Weijie Qin' 'Wantao Ying' 'Yanchang Li' 'Cheng Chang' 'Xiaowen Wang' 'Linhai Xie' 'Wei Liu' 'Jialin Liu' 'Xiaomei Zhang' 'Qunjiao Yan' 'Yu Zou' 'Chuanping Zhao' 'Haofan Sun' 'Jian Zhang' 'Shicheng Su' 'Qiang Gao' 'Fuchu He'] Title: ABSTRACT The concept of ‘proteomics-driven precision medicine’ highlighted in 2019…
Chiara Bernardini, Maike Däther, Franziska R. Traube
Spatial proteomics has emerged as a powerful approach to systematically map the subcellular localization of thousands of proteins in parallel, providing insights into organelle composition, protein trafficking, and context-dependent relocalization events. Building on advances in mass spectrometry sensitivity, and…
Yi-Chien Wu, Dylan Schwartz, Elie Abi Khalil, Aditi Upadhye + 2 more
Spatial proteomics uncovers the molecular basis of cellular function in intact tissues. Laser capture microdissection coupled with mass spectrometry enables comprehensive proteomic profiling of selected tissue regions but typically does not support cell-type-specific proteomic analysis. We present NicheProt, a 3D…
Georg H. H. Borner
Protein subcellular localization is highly regulated and critical for protein function. Spatial proteomics aims at capturing the localization dynamics of all proteins expressed in a given cell type. Among different approaches, organellar mapping through proteomic profiling stands out as the only method capable of…
Yumi Kwon, Jongmin Woo, Fengchao Yu, Sarah M. Williams + 9 more
Multiplexed bimolecular profiling of tissue microenvironment, or spatial omics, can provide deep insight into cellular compositions and interactions in healthy and diseased tissues. Proteome-scale tissue mapping, which aims to unbiasedly visualize all the proteins in a whole tissue section or region of interest, has…
Ching-Chia Yang, Ching-Ya Lin, Hsin-Yo Yuan, Hsuan-Cheng Huang + 1 more
Mass spectrometry-based spatial omics is a powerful approach for visualizing the spatial organization of proteins, metabolites, lipids, and other biomolecules in situ, combining the molecular depth of mass spectrometry with spatially resolved imaging. This systematic review traces the rapid technological and…
Yumi Kwon, Jongmin Woo, Fengchao Yu, Sarah M. Williams + 9 more
Multiplexed bimolecular profiling of tissue microenvironment, or spatial omics, can provide deep insight into cellular compositions and interactions in healthy and diseased tissues. Proteome-scale tissue mapping, which aims to unbiasedly visualize all the proteins in a whole tissue section or region of interest, has…
Stephen Coleman, Lisa Breckels, Ross F. Waller, Kathryn S. Lilley + 4 more
The subcellular localisation of proteins is a key determinant of their function. High-throughput analyses of these localisations can be performed using mass spectrometry-based spatial proteomics, which enables us to examine the localisation and relocalisation of proteins. Furthermore, complementary data sources can…
Tarun Mishra, Ritudhwaj Tiwari, Tuyelee Das, Maneesh Lingwan + 4 more
As global ecosystems and food systems face unprecedented anthropogenic and climatic challenges, there is a demand for an integrated understanding of biological systems. Proteomics has emerged as a definitive approach offering a direct view of the molecular phenotype, yet it is traditionally separated into plant and…
Laurent Gatto, Lisa M. Breckels, Kathryn S. Lilley
The sub-cellular localisation of a protein is vital in defining its function, and a protein's mis-localisation is known to lead to adverse effect. As a result, numerous experimental techniques and datasets have been published, with the aim of deciphering the localisation of proteins at various scales and resolutions…
Matineh Rahmatbakhsh, Alla Gagarinova, Mohan Babu
Microbial pathogens have evolved numerous mechanisms to hijack host’s systems, thus causing disease. This is mediated by alterations in the combined host-pathogen proteome in time and space. Mass spectrometry-based proteomics approaches have been developed and tailored to map disease progression. The result is complex…
Olanrewaju Ayodeji Durojaye
Specialized biological processes occur in different regions and organelles of the cell. Additionally, the function of proteins correlate greatly with their interactions and subcellular localization. Understanding the mechanism underlying the specialized functions of cellular structures therefore requires a detailed…
Vincent Geoghegan, Jeremy C. Mottram, Nathaniel G. Jones
Proximity labelling is a powerful and rapidly developing technology for exploring the interaction space and molecular environment of a protein of interest at the nanometre scale. In proximity labelling, a promiscuous biotinylating enzyme is genetically fused to the protein of interest, initiation of labelling then…