12 papers · ranked by Valyu relevance
Jahnavi Bhaskaran, Elizabeth Ing-Simmons, Irina Balaguer Balsells, Sara Di Persio + 11 more
Gametogenesis, the production of oocytes and sperm, ensures the faithful transmission of genetic material to the next generation. In males, spermatogenesis occurs within the testis, where germ cells progress through a highly ordered developmental programme in close association with supporting somatic cells. Defects in…
Ru Yu, Min Gao, Lin Cai, Shuoxuan Wang + 8 more
Key message StERF87 directly activates StPR1a via GCC-box binding and integrates SA/ET signaling to enhance bacterial wilt resistance. Abstract Potato (Solanum tuberosum) is an important food crop worldwide, yet its yield is severely constrained by bacterial wilt caused by Ralstonia solanacearum. We used RNA-Seq to…
Xing Gan, Jianting Zhang, Longbo Liu, Liuzi Zhang + 3 more
5-Aminolevulinic acid (ALA) is an emerging plant growth regulator, yet its mechanisms in fruit development remain unclear. Here, we show that ALA promotes early growth and ultimate size in peach (Prunus persica) fruits. ALA treatment significantly enhanced the mesocarp area and the cell number, accompanied by elevated…
Huihai Wu, Ashleigh Lister, Iain Macaulay, Katie Long + 10 more
Single-cell and spatial transcriptomics are transforming our understanding of cellular heterogeneity and tissue organization, yet their analytical complexity remains a major bottleneck. Here, we present EISCA and EISTA, two standardized, end-to-end pipelines for single-cell RNA- seq and imaging-based spatial…
Yusuke Tsuruta, Hirotaka Inoue, Kenta Hagiwara, Charles S Hoffman + 1 more
An appropriate transcriptional response to external stresses is crucial for all organisms. Gene expression is regulated by transcription factors (TFs) binding to specific DNA cis-elements. However, how individual TFs achieve stress-specific binding remains elusive. Here we examine the molecular basis of the…
Alejandro Espinoza, Bruno J. de Andrade Silva, Matteo Pellegrini
Single-cell RNA sequencing technologies provide insights into gene expression at the cellular level, enabling detailed analysis of cellular heterogeneity. In this study, we systematically compared two scRNA-seq platforms,10X Genomics and Parse Biosciences, using human peripheral blood mononuclear cells (PBMCs) and…
Timothy D. Martin, Mei Yuk Choi, Julia C. McBride, Stephen J. Elledge
Title: Significance While KRAS mutations drive a significant portion of human malignancies, their prevalence is strikingly restricted to specific lineages, namely pancreatic, colorectal, and lung tissues. This tissue-restricted tropism suggests that oncogenic KRAS does not operate in a vacuum but requires a permissive…
Federico Vannuccini, Yari Ciani, Vera Mugoni, Orsetta Quaini + 20 more
Circulating analytes in cancer patients capture tumor-related signals. We profiled matched extracellular vesicle (EV) total RNA and cell-free DNA (cfDNA) from the same plasma aliquots of chemo-naive metastatic castration-resistant prostate cancer (mCRPC) patients treated with Enzalutamide (n=54 patients, n=119…
E. Kashuk, A. Tarakanova, A. Malygina, N. Kuzovkina + 4 more
SIRT6 is a NAD^+^-dependent sirtuin that plays central roles in chromatin regulation, DNA repair, telomere maintenance, metabolic homeostasis, and inflammatory control. Although SIRT6 have long been implicated in aging because of its association with several hallmarks of aging, the available evidence remains largely…
Ye Gao, Ni Wang, Zhaoyang Xiao, Alessandro Medoro
Acute lung injury (ALI) and acute respiratory distress syndrome (ARDS) caused by sepsis are common and critical conditions characterized by severe respiratory distress and refractory hypoxemia . ARDS, a severe manifestation of ALI, is one of the leading causes of respiratory failure and multiple organ dysfunction…
Bryan Ramirez-Corona, Santiago Cadena, Kerry Bubb, Christine Queitsch + 1 more
In nature, plants experience complex combinations of environmental stresses. Prior studies using Arabidopsis thaliana show that transcriptional responses to combinatorial stress are unique and cannot be predicted from responses to individual treatments, making it imperative to study combinatorial stress in the…
Jon Wallner, Asanda Mazubane, Jaap van der Heijden, Egor Vorontsov + 4 more
Septic shock represents the most severe form of infection-driven systemic inflammation, whereas acute pancreatitis induces a sterile inflammatory response. Although clinically similar, their molecular profiles may reveal distinct mechanisms underlying infectious and non-infectious inflammation. We performed plasma…