12 papers · ranked by Valyu relevance
Adam Stovicek, Murat Serhatlioglu, Babak Rezaei, Stephan Sylvest Keller + 4 more
We demonstrate the first viscoelastic-based flow-integrated Raman spectroscopy (FIRS) platform using spontaneous Raman spectroscopy with a simplified fluidic design that precisely controls particle transit speed and predicts arrival time to the Raman interrogation region, together with time synchronized triggered…
Rong Tang, Jiaze Yin, Bethany Weinberg, Haonan Lin + 4 more
By optically sensing mid-infrared absorption through a visible probe beam, mid-infrared photothermal (MIP) microscopy has emerged as a powerful tool for chemical imaging with micromolar sensitivity and submicron spatial resolution. The adoption of spatially multiplexed camera-based widefield detection further enhanced…
Anna Lena Schäfer, Cristina Gellini, Rolf Diller, Katrina T. Forest + 2 more
Time-resolved resonance Raman spectroscopy with continuous-wave excitation is a fundamental technique that has contributed substantially to the understanding of structure and dynamics of bacteriorhodopsin and related retinal proteins. However, the underlying principles were developed about fifty years ago for…
Shun Arai, Shogo Matsubara, Toru Kondo
Photosynthetic light harvesting proceeds rapidly and robustly through precisely arranged pigment molecules. However, the molecular arrangements are not uniform because they exhibit structural heterogeneity among individual complexes and across spatial regions, and undergo dynamic fluctuations. Such static and dynamic…
J. Demas, L. Tan, S. Ramachandran
The performance of a laser scanning microscope inevitably depends on the performance of the point detector. As laser scanning approaches aim to penetrate deeper in tissue, there is a commensurate need for detectors that can operate with high sensitivity, bandwidth, and dynamic range at near-infrared wavelengths where…
Lynn M. Sidor, Kathren P. Sage, Michelle M. Beaulieu, Dylan F. Wilson + 6 more
Recently, engineered bacterial cells have been shown to behave as optically-active photonic devices comparable to industrially fabricated microlenses^1^. Bacterial cells can be encapsulated within a layer of polysilicate through surface display of the sea sponge enzyme silicatein, which mineralizes a polysilicate…
Yuechuan Lin, Xiang Zhang, Rebecca E Zubajlo, Zahid Yaqoob + 2 more
Quantitative phase microscopy (QPM) enables label-free imaging of structure and dynamics in biological and physical systems, yet achieving high-speed three-dimensional (3D) QPM with strong optical sectioning remains a central challenge. Here, we introduce a single-shot reflection-mode temporal focusing QPM (TF-QPM)…
Yanfan Zhu, Marilyn M. Lionts, Ezekiel J. Haugen, Alec B. Walter + 10 more
Raman spectroscopy offers a uniquely rich window into molecular structure and composition, making it a powerful tool across fields ranging from materials science to biology. However, the reproducibility of Raman data analysis remains a fundamental bottleneck. In practice, transforming raw spectra into meaningful…
Liam Shaughnessy, Lauren Vannell, Sulekh Fernando-Peiris, Cristina Rodríguez
Three-photon microscopy enables deep-tissue imaging but is highly sensitive to excitation pulse quality, since three-photon excitation efficiency scales with the cube of instantaneous intensity. Group-delay dispersion (GDD) and third-order dispersion (TOD) accumulated through the laser and microscope optics can…
Drew Ehrlich, Yohei Rosen, Santhosh Arul, John Minnick + 16 more
Longitudinal live cell imaging is valuable for characterizing dynamic morphological and phenotypic changes in biological systems. How-ever, conventional approaches rely on manual microscope operation, which is labor-intensive, limits imaging frequency, and disrupts the cellular environment. These constraints reduce…
Jiawang Qiu, Tao Yuan, Francesca Gasparin, Miguel A. Pleitez
Cellular metabolic activities can be studied using label-free vibrational spectroscopic imaging to leverage the endogenous contrast of biomolecules. However, fast live-cell imaging over large fields-of-view remains challenging due to the need for raster scanning and despite advances in wide-field modalities, imaging…
Peng Chen, Keyi Han, Zijun Gao, Claire M. Deng + 8 more
Multimode fibers enable minimally invasive, high-resolution imaging through ultrathin probes, thereby enhancing diagnostic precision and facilitating real-time monitoring in delicate anatomical regions. In this work, we introduce HYFEN, a hydrogel-based endomicroscopic imaging platform for flexible, biocompatible, and…