Optophysiology: Illuminating cell physiology with optogenetics TAN ET AL. OPTOGENETICS FOR PRECISE INTERROGATION OF CELL PHYSIOLOGY

Abstract

Optogenetics is also emerging as a unique technology to accelerate the pace of drug screening. High-throughput screens built upon pooled libraries for CRISPR-Cas9, base editing, or chemicals have tremendously contributed to the discovery of novel gene function, signaling networks, and pathways of action. In situ sequencing-based optical pooled screens that couple genetic screens with phenotypic screens allow the identification of causal relationships between perturbations and phenotypes (409). However, perturbations in conventional pooled screens are often triggered by ligands or chemicals, wh