29 papers · ranked by Valyu relevance
Akash Guru, Ryan J Post, Yi-Yun Ho, Melissa R Warden
This review, one of a series of articles, tries to make sense of optogenetics, a recently developed technology that can be used to control the activity of genetically-defined neurons with light. Cells are first genetically engineered to express a light-sensitive opsin, which is typically an ion channel, pump, or G…
E. A. Claudia Pama, Lorenza S. Colzato, Bernhard Hommel
Optogenetics may be the answer to a search for temporal and spatial specificity in neuroscience. The well-known trade-off between temporal and spatial specificity might be resolved with this “combination of genetic and optical methods to achieve gain or loss of function of well-defined events in specific cells of…
Hamid Gholami Pourbadie, Mohammad Sayyah
Neuronal cells communicate with each other by producing electrical signals or action potentials (APs). Different ion channels, including Na+, K+ and Ca2+ channels, are involved in generation of AP. Once an AP is generated in the soma, it travels down entire the axon length toward its terminal in a self-generating…
Alexandra-Madelaine Tichy, Elliot J. Gerrard, Julien M.D. Legrand, Robin M. Hobbs + 1 more
Optogenetics enables the spatio-temporally precise control of cell and animal behaviour. Many optogenetic tools are driven by light-controlled protein-protein-interactions (PPIs) that are repurposed from natural light-sensitive domains (LSDs). Applying light-controlled PPI to new target proteins is challenging because…
Robel Dagnew, Yin-Ying Lin, Jerikko Agatep, Michael Cheng + 9 more
'Andrew Jann' 'Viola Quach' 'Michelle Monroe' 'Ganeev Singh' 'Ani Minasyan' 'Joshua Hakimian' 'Theodore Kee' 'Jesse Cushman' 'Wendy Walwyn'] Title: Abstract. The use of optogenetics to activate or inhibit neurons is an important toolbox for neuroscientists. Several optogenetic devices are in use. These range from wired…
Tianyu Terry Gao, Teak‐Jung Oh, Kritika Mehta, Yu‐En Andrew Huang + 5 more
1## BACKGROUND Insights into pathogenesis are crucial for disease prevention, diagnosis, management, and treatment. Disease etiology and progression often involve disruptive gene modification, defective intracellular and intercellular cell signaling, and dysregulated cell-environment communications, which could…
D. V. Kolesov, E. L. Sokolinskaya, K. A. Lukyanov, A. M. Bogdanov
In modern life sciences, the issue of a specific, exogenously directed manipulation of a cell’s biochemistry is a highly topical one. In the case of electrically excitable cells, the aim of the manipulation is to control the cells’ electrical activity, with the result being either excitation with subsequent generation…
Zoé Christenson Wick, Esther Krook-Magnuson
Optogenetics is a powerful and rapidly expanding set of techniques that use genetically encoded light sensitive proteins such as opsins. Through the selective expression of these exogenous light-sensitive proteins, researchers gain the ability to modulate neuronal activity, intracellular signaling pathways, or gene…
Steven Husson, Alexander Gottschalk, Andrew M. Leifer
The emerging field of optogenetics allows for optical activation or inhibition of neurons and other tissue in the nervous system. In 2005 optogenetic proteins were expressed in the nematode C. elegans for the first time. Since then, C. elegans has served as a powerful platform upon which to conduct optogenetic…
Inka Busack, Florian Jordan, Peleg Sapir, Henrik Bringmann
Optogenetics controls neural activity and behavior in living organisms through genetically targetable actuators and light. This method has revolutionized biology and medicine as it allows controlling cells with high temporal and spatial precision. Optogenetics is typically applied only at short time scales, for…
Kirti Sharma, Zoe Jaeckel, Artur Schneider, Oliver Paul + 2 more
Optogenetics involves delivery of light-sensitive opsins to the target brain region, as well as introduction of optical and electrical devices to manipulate and record neural activity, respectively, from the targeted neural population. Combining these functionalities in a single implantable device is of great…
Rachel Berry, Matthew Getzin, Lars Gjesteby, Ge Wang
Optogenetics as developed by Dr. Karl Deisseroth and others has been a transformative technology in the area of neuroscience. By stimulating genetically-modified neurons with visible light, modulation of the ionic conduction across the cell membrane can be achieved with very high specificity and precise temporal…
Zachary Quine, Alexei Goun, Karl P. Gerhardt, Jeffrey J. Tabor + 1 more
'Herschel Rabitz'] Optogenetics is a revolutionary new field of biotechnology, achieving optical control over biological functions in living cells by genetically inserting light sensitive proteins into cellular signaling pathways. Applications of optogenetic switches are expanding rapidly, but the technique is hampered…
Álvaro Inglés-Prieto, Nikolas Furthmann, Samuel Crossman, Nina Hoyer + 9 more
Optogenetics has been harnessed to shed new mechanistic light on current therapies and to develop future treatment strategies. This has been to date achieved by the correction of electrical signals in neuronal cells and neural circuits that are affected by disease. In contrast, the optogenetic delivery of trophic…
Niklas Smedemark-Margulies, Josef G. Trapani
The advent of optogenetics and genetically encoded photosensors has provided neuroscience researchers with a wealth of new tools and methods for examining and manipulating neuronal function in vivo. There exists now a wide range of experimentally validated protein tools capable of modifying cellular function, including…
Karl P. Gerhardt, Evan J. Olson, Sebastian M. Castillo-Hair, Lucas A. Hartsough + 8 more
In optogenetics, researchers use light and genetically encoded photoreceptors to control biological processes with unmatched precision. However, outside of neuroscience, the impact of optogenetics has been limited by a lack of user-friendly, flexible, accessible hardware. Here, we engineer the Light Plate Apparatus…
Hidenori Watanabe, Hiromi Sano, Satomi Chiken, Kenta Kobayashi + 4 more
Optogenetics has become an indispensable tool for investigating brain functions. Although non-human primates are particularly useful models for understanding the functions and dysfunctions of the human brain, application of optogenetics to non-human primates is still limited. In the present study, we generated an…
Lizhu Li, Lihui Lu, Yuqi Ren, Guo Tang + 12 more
The precise control of neural activities at both cellular and circuit levels reveals significant impacts on the fundamental neuroscience explorations and medical applications. Optogenetic methods provide efficient cell-specific modulations, but state-of-the-art technologies lack the ability of simultaneous neural…
Fereshteh Arab, Sareh Rostami, Mohammad Dehghani-Habibabadi, Vahid Salari + 1 more
'Vahid Salari' 'Mir-Shahram Safari'] It has been hypothesized that Gamma cortical oscillations play important roles in numerous cognitive processes and may involve psychiatric conditions including anxiety, schizophrenia, and autism. Gamma rhythms are commonly observed in many brain regions during both waking and sleep…
Rishi Rajalingham, Michael Sorenson, Reza Azadi, Simon Bohn + 2 more
Challenges in behavioral optogenetics in large brains demand development of a chronically implantable platform for light delivery. We have developed Opto-Array, a chronically implantable array of LEDs for high-throughput optogenetic perturbation in non-human primates. We tested the Opto-Array in the primary visual…
Jonghee Yoon, Minji Lee, KyeoReh Lee, Nury Kim + 5 more
'Jongchan Park' 'Chulhee Choi' 'Won Do Heo' 'YongKeun Park'] **Jonghee Yoon1,†, Minji Lee2, † , KyeoReh Lee1 , Nury Kim2 , Jin Man Kim3 , Jongchan Park1 , Hyeonseung Yu1 , Chulhee Choi4 , Won Do Heo2,5, and YongKeun Park1,** 1 Department of Physics, Korea Advanced Institute of Science and Technology, Daejeon 305-701…
Riichiro Hira, Yoshikazu Isomura
We thank Dr. K. Isobe at RIKEN for discussion. This work was supported by JP22wm0525007 (RH), JP19dm0207089 (YI) from AMED, JP22H02731 (RH), JP20K22678 (RH), JP21B304 (RH), JP21H05134 (RH), JP21H05135 (RH), JP21H0524 2(YI), and JP23H02589 (YI) from MEXT/JSPS, JPMJCR1751 (YI) from JST, Nakatani Foundation (RH), Shimadzu…
Authors not listed
Channelrhodopsin-2 (ChR2) is a light-gated ion channel widely used in optogenetics, a technique that enables precise control of neuronal activity by genetically engineering light-sensitive proteins into cell membranes. This protein exists in dimeric form, with each monomer containing a retinal Schiff base (RSB) moiety…
Nan Zheng, Ying Jiang, Shan Jiang, Jongwoon Kim + 4 more
'Ji‐Xin Cheng' 'Xiaoting Jia' 'Chen Yang'] C.Y. conceived the project. N.Z. and S.J. performed fabrication and characterization of materials. N.Z. and Y.J. performed the stimulation and recording experiments in vitro and in vivo. N.Z. and Y.L. performed the in vivo biocompatibility evaluations. X.J. provided guidance…
Spencer Kim, Dylan Gray, Michelle Shanguhyia, Rachel Steinhardt
Recreating the signaling profile a chemical synapse to analyze serotonin receptor activation is a challenge. This is due in part to the kinetics of the synapse, where neurotransmitters are rapidly released and quickly cleared by active reuptake machinery. One strategy to produce a rapid rise in a bio-orthogonally…
Giacomo Mazzotti, Denis Hartmann, Michael Booth
Cell-free expression of a gene to protein has become a vital tool in nanotechnology and synthetic biology. Remote-control of cell-free systems with multiple, orthogonal wavelengths of light would enable precise, non-invasive modulation, opening many new applications in biology and medicine. While there has been success…
Afsaneh Taheri Kal-Koshvandi
We present the first light-free, genetically minimal platform for spatiotemporally resolved proximity labeling across five neuronal compartments—synaptic, nuclear, endosomal, mitochondrial, and activity-dependent microdomains. A covalent "catalyst–luciferase sandwich" architecture site-specifically conjugates five…
Authors not listed
Photocages have emerged as powerful tools for enabling precise control over the timing, location and quantity of the released biomolecule of interest. However, most commonly used photocages require activation by UV or violet light, which is cytotoxic. Moreover, the exclusive control of light on their photoreactivity…
Authors not listed
Spatiotemporal control of molecular release is essential for the precise manipulation of cellular functions, and photo-stimulation is one of the most widely used strategies to achieve this control. However, direct light exposure can cause cell damage due to the generation of reactive oxygen species and photothermal…