24 papers · ranked by Valyu relevance
Barnali Waugh, Sharon G. Wolf, Deborah Fass, Eric Branlund + 3 more
The complex environment of biological cells and tissues has motivated development of three dimensional imaging in both light and electron microscopies. To this end, one of the primary tools in fluorescence microscopy is that of computational deconvolution. Wide-field fluorescence images are often corrupted by haze due…
Peter Kirchweger, Lev Melnikovsky, Shahar Seifer, Michael Elbaum
Cryo-electron tomography is an expanding technology for the study of macromolecules, viruses, and cells. It is often applied to specimens that are too large or heterogeneous for methods based on 2D image averaging such as single particle analysis, e.g., intracellular membranes or organelles. Current practice records a…
Hayato Ikoma, Michael Broxton, Takamasa Kudo, Gordon Wetzstein
Deconvolution is widely used to improve the contrast and clarity of a 3D focal stack collected using a fluorescence microscope. But despite being extensively studied, deconvolution algorithms can introduce reconstruction artifacts when their underlying noise models or priors are violated, such as when imaging…
Tatiana Latychevskaia
| Abstract 2 | | --- | | 1. Introduction 2 | | 2. 3D volumetric deconvolution in coherent optics 3 | | 2.1 Principles of image formation by convolution 3 | | 2.2 Analytical wavefront from a point-like source 4 | | 2.3 Simulated wavefront from a point-like source 5 | | 3. Deconvolution algorithms 7 | | 3.1 Non-iterative…
Soonam Lee, Shuo Han, Paul Salama, Kenneth W. Dunn + 1 more
Due to image blurring image deconvolution is often used for studying biological structures in fluorescence microscopy. Fluorescence microscopy image volumes inherently suffer from intensity inhomogeneity, blur, and are corrupted by various types of noise which exacerbate image quality at deeper tissue depth. Therefore…
Junseong Eom, Sangjun Moon
The digital in-line holographic microscope (DIHM) was developed for a 2D imaging technology and has recently been adapted to 3D imaging methods, providing new approaches to obtaining volumetric images with both a high resolution and wide field-of-view (FOV), which allows the physical limitations to be overcome.…
Zhenhong Du, Jiajia Chen, Cancan Gao, Jiayu Li + 4 more
Fluorescence images obtained with optical microscopes intrinsically suffer from blur and noise, which can be partially reversed by the deconvolution process. However, the deconvolution process is ill-conditioned, leading to a trade-off between detail preservation and noise suppression. Here, we develop 3D-FUDIP to…
Sylvain Prigent, Hoai-Nam Nguyen, Ludovic Leconte, Cesar Augusto Valades-Cruz + 3 more
While fluorescent microscopy imaging has become the spearhead of modern biology as it is able to generate long-term videos depicting 4D nanoscale cell behaviors, it is still limited by the optical aberrations and the photon budget available in the specimen and to some extend to photo-toxicity. A direct consequence is…
Boyoung Kim, Doug Brooks, Alexandra Sorvina, Shane Hickey
To investigate the cellular structure, biomedical researchers often obtain three-dimensional images by combining two-dimensional images taken along the z axis. However, these images are blurry in all directions due to diffraction limitations. This blur becomes more severe when focusing further inside the specimen as…
Boyoung Kim, Takeshi Naemura
This paper proposes a new deconvolution method for 3D fluorescence wide-field microscopy. Most previous methods are insufficient in terms of restoring a 3D cell structure, since a point spread function (PSF) is simply assumed as depth-invariant, whereas a PSF of microscopy changes significantly along the optical axis.…
Yuanzheng Ma, Chang Lu, Kedi Xiong, Wuyu Zhang + 1 more
A micro-electromechanical system (MEMS) scanning mirror accelerates the raster scanning of optical-resolution photoacoustic microscopy (OR-PAM). However, the nonlinear tilt angular-voltage characteristic of a MEMS mirror introduces distortion into the maximum back-projection image. Moreover, the size of the airy disk…
Paul Joseph Tadrous
Anatomy has advanced using 3-dimensional (3D) studies at macroscopic (e.g., dissection, injection moulding of vessels, radiology) and microscopic (e.g., serial section reconstruction with light and electron microscopy) levels. This paper presents the first results in human cells of a new method of subcellular 3D…
Mikhail Papkov, Kaupo Palo, Leopold Parts
Inverse problems in image reconstruction are fundamentally complicated by unknown noise properties. Classical iterative deconvolution approaches amplify noise and require careful parameter selection for an optimal trade-off between sharpness and grain. Deep learning methods allow for flexible parametrization of the…
Chenyu Xu, Zhouyu Jin, Chengkang Shen, Hao Zhu + 5 more
'You Zhou' 'Xun Cao' 'Ning Gu'] Abstract. Compared to light-field microscopy (LFM), which enables high-speed volumetric imaging but suffers from non-uniform spatial sampling, Fourier light-field microscopy (FLFM) introduces sub-aperture division at the pupil plane, thereby ensuring spatially invariant sampling and…
Canyu Yang, Dennis Eschweiler, Johannes Stegmaier
Recent developments in fluorescence microscopy allow capturing high-resolution 3D images over time for living model organisms. To be able to image even large specimens, techniques like multi-view light-sheet imaging record different orientations at each time point that can then be fused into a single high-quality…
Amit Kohli, Anastasios N. Angelopoulos, David McAllister, Esther Whang + 6 more
'Esther Whang' 'Sixian You' 'Kyrollos Yanny' 'Federico M. Gasparoli' 'Bo-Jui Chang' 'Reto Fiolka' 'Laura Waller'] The most ubiquitous form of aberration correction for microscopy is deconvolution; however, deconvolution relies on the assumption that the system’s point spread function is the same across the entire field…
Long Pan, Yunan Wang, Yijie Lou, Xiaohua Feng
thick scattering medium Authors: ['Long Pan' 'Yunan Wang' 'Yijie Lou' 'Xiaohua Feng'] Abstract: Imaging through thick scattering media presents significant challenges, particularly for three-dimensional (3D) applications. This manuscript demonstrates a novel scheme for single-image-enabled 3D imaging through such…
Jixiong Su, Shoupei Liu, Shasha Yang, Yanfeng Zhu + 7 more
Three-photon microscopy (3PM) has extended optical imaging beyond the reach of two-photon microscopy (2PM), but its practical depth remains far below theoretical predictions because of low photon efficiency and severe noise contamination. Here we present an integrated hardware and software solution that addresses these…
Adeleke Maradesa, Baptiste Py, Ting Hei Wan, Mohammed B. Effat + 1 more
Electrochemical impedance spectroscopy (EIS) is a characterization technique used widely in electrochemistry. Obtaining EIS data is simple when modern electrochemical workstations are used; however, analyzing EIS spectra is still a considerable quandary. The distribution of relaxation times (DRT) has emerged as a…
Kelsey Hatzell, Yanjie Zheng
X-ray Computed Tomography (CT) is a non-invasive, non-destructive approach to imaging materials, material systems and engineered components in two- and three- dimensions. Acquisition of 3D images requires the collection of hundreds or thousands of through-thickness X-ray radiographic images from different angles. Such…
Amelia Carolina Sparavigna
Here we consider calcite and its spectra (Raman and infrared) that we can find in the RRUFF database. We apply q-Gaussian and q-BWF functions to decompose the spectra. The q-Gaussian functions had been derived in the framework of Tsallis analysis of entropy. Their use for Raman spectroscopy has been proposed by…
Authors not listed
Scanning emission-based microscopies, such as X-ray fluorescence (XRF) and energy-dispersive X-ray spectroscopy, offer nanometer-scale chemical maps, but suffer from long acquisition times and radiation damage. Lower-flux and shorter dwell time scans mitigate this problem, but the resulting signal loss can only…
Amelia Carolina Sparavigna
Natrolite, Na2(Si3Al2)O10·2H2O, is a member of a group of zeolite minerals, made of hydrated aluminosilicate minerals. Due to their structure, zeolites have channels, through which water and ions can move. In the RRUFF database, we find the following members of the group: natrolite, mesolite, scolecite, and gonnardite.…
Denice van Herwerden, Jake O'Brien, Sascha Lege, Bob Pirok + 2 more
Fragment deconvolution is a crucial step during componentization of non-targeted analysis (NTA) high-resolution mass spectrometry (HRMS) data, aiming to filter out false positive (FP) signals that do not belong to the component. Moreover, inclusion of FP fragments could lead to, for example, wrong identification…