25 papers · ranked by Valyu relevance
Yang Zhang, Laura Garcia‐Ibanez, Kai‐Michael Toellner
Title: Summary Germinal centers (GC) are the main sites where antigen-activated B-cell clones expand and undergo immunoglobulin gene hypermutation and selection. Iterations of this process will lead to affinity maturation, replicating Darwinian evolution on the cellular level. GC B-cell selection can lead to four…
Felix M. Wensveen, Erik Slinger, Martijn HA van Attekum, Robert Brink + 1 more
'Robert Brink' 'Eric Eldering'] Upon antigen encounter, the responsive B cell pool undergoes stringent selection which eliminates cells with low B cell receptor (BCR) affinity. Already before formation of the germinal center, activated B cells of low-affinity are negatively selected in a process that is molecularly not…
Eglantine Hector, Pierre Milpied
Germinal centres (GCs) are specialized structures where B cells undergo iterative steps of B-cell receptor (BCR) somatic hypermutation and selection of best antigen binders in a darwinian-like fashion. The accelerated evolutionary process leads to the production of high-affinity antibodies that are crucial for robust…
Vinod Krishna, Kurtis E. Bachman, Stephen J Turner
A model of B cell affinity selection is proposed, and an explanation of peripheral tolerance mechanisms through antibody repertoire editing is presented. We show that affinity discrimination between B cells is driven by a competition between obtaining T cell help and removal of B cells from the light zone, either…
Vinod Krishna, Kurtis E. Bachman
A model of B cell affinity selection is proposed, and an explanation of peripheral tolerance mechanisms through antibody repertoire editing is presented. We show that affinity discrimination between B cells is driven by a competition between obtaining T cell help and removal of B cells from the light zone, either…
Stephen Lindsly, Maya R. Gupta, Alnawaz Rehemtulla, Indika Rajapakse
The mammalian adaptive immune system has evolved over millions of years to become an incredibly effective defense against foreign antigens. The adaptive immune system's humoral response creates plasma B cells and memory B cells, each with their own immunological objectives. The affinity maturation process is widely…
Tamás Szabados, Csaba Kerepesi, Tibor Bakács
Our main purpose is to compare classical nonself-centered, two-signal theoretical models of the adaptive immune system with a novel, self-centered, one-signal model developed by our research group. Our model hypothesizes that the immune system of a fetus is capable learning the limited set of self antigens but unable…
David R. Glass, Albert G. Tsai, John Paul Oliveria, Felix J. Hartmann + 7 more
'Felix J. Hartmann' 'Samuel C. Kimmey' 'Ariel A. Calderon' 'Luciene Borges' 'Marla C. Glass' 'Lisa E. Wagar' 'Mark M. Davis' 'Sean C. Bendall'] Title: Summary B cells are capable of a wide range of effector functions including antibody secretion, antigen presentation, cytokine production, and generation of…
Lisa Buchauer, Hedda Wardemann
Germinal centres are anatomically defined lymphoid organ structures that mediate B cell affinity maturation and affect the quality of humoral immune responses. Mathematical models based on differential equations or agent-based simulations have been widely used to deepen our understanding of the cellular and molecular…
Ahmed M. Alhassan, Venktesh S. Shirure, Jean Luo, Bryan B. Nguyen + 5 more
Assessing B cell affinity to pathogen-specific antigens prior to or following exposure could facilitate the assessment of immune status. Current standard tools to assess antigen-specific B cell responses focus on equilibrium binding of the secreted antibody in serum. These methods are costly, time-consuming, and assess…
Tamás Szabados, Gábor Tusnády, László Varga, Tibor Bakács
Many events in the vertebrate immune system are influenced by some element of chance. The objective of the present work is to describe affinity maturation of B lymphocytes (in which random events are perhaps the most characteristic), and to study a possible network model of immune memory. In our model stochastic…
Rajat Desikan, Rustom Antia, Narendra M. Dixit
The affinity of antibodies (Abs) produced in vivo for their target antigens (Ags) is typically well below the maximum affinity possible. Nearly 25 years ago, Foote and Eisen explained how an 'affinity ceiling' could arise from constraints associated with the acquisition of soluble antigen by B cells. However, recent…
David R. Glass, Albert G. Tsai, John Paul Oliveria, Felix J. Hartmann + 4 more
To evaluate the impact of heterogeneous B cells in health and disease, comprehensive profiling is needed at a single cell resolution. We developed a highly-multiplexed screen to quantify the co-expression of 351 surface molecules on low numbers of primary cells. We identified dozens of differentially expressed…
Ana Martínez-Riaño, Pilar Delgado, Pilar Mendoza, Clara L. Oeste + 4 more
Successful vaccines rely on activating a functional humoral response that results from generating class-switched high affinity immunoglobulins (Igs) with a superior capacity to neutralize infection. Key to this process is the germinal center (GC) reaction, in which B cells are selected in their search for antigen and T…
Dale. O Starkie, Joanne E. Compson, Stephen Rapecki, Daniel J. Lightwood + 1 more
'Daniel J. Lightwood' 'Sophia N Karagiannis'] Single B cell screening strategies, which avoid both hybridoma fusion and combinatorial display, have emerged as important technologies for efficiently sampling the natural antibody repertoire of immunized animals and humans. Having access to a range of methods to…
Philippos K. Tsourkas, Somkanya C Das, Paul Yu-Yang, Wanli Liu + 2 more
'Susan K. Pierce' 'Subhadip Raychaudhuri'] B cells encounter antigen over a wide affinity range, from KA=105 M-1 to KA=1010 M-1 . The strength of B cell signaling in response to antigen increases with affinity, a process known as "affinity discrimination". In this work, we use a computational simulation of B cell…
Giovanna Clavarino, Noémie Delouche, Claire Vettier, David Laurin + 6 more
'Martine Pernollet' 'Tatiana Raskovalova' 'Jean-Yves Cesbron' 'Chantal Dumestre-Pérard' 'Marie-Christine Jacob' 'R. Keith Reeves'] A precise identification and phenotypic characterization of human B-cell subsets is of crucial importance in both basic research and medicine. In the literature, flow cytometry studies for…
Xiujia Yang, Haipei Tang, Chunhong Lan, Weiting He + 6 more
The development and maturation of B lymphocytes involve intricate orchestrated processes, where dedicated gene regulations (GR) take place within specific microenvironments shaped by both extracellular matrix and neighboring cells. Despite extensive investigations aimed at deepening our comprehension of these…
Authors not listed
In many autoimmune pathologies, including Rheumatoid Arthritis (RA), only a small percentage of the total B cell population is autoreactive and sustain disease. Yet, current immunotherapy treatments often eliminate the entire B-cell population, leading to immune deficiency. We developed an approach to selectively…
Ulrike Strittmatter-Keller, Caroline Walter, Celine Rauld, Nicole Egli + 6 more
Differentiation of B cells is a stringently controlled multi-step process, which is still incompletely understood. Here we identify and characterize a rare population of human B cells, which surprisingly carry CD8AB on their surface. Existence of such cells was demonstrated both in tonsils and in human apheresis…
Caroline Montaudouin, Marie Anson, Yi Hao, Susanne V. Duncker + 8 more
'Tahía D. Fernández' 'Emmanuelle Gaudin' 'Michael R. Ehrenstein' 'William G. Kerr' 'Jean‐Hervé Colle' 'Pierre Bruhns' 'Marc Daëron' 'António A. Freitas'] 1 1 This work was supported by grants from the European Research Council (ERC), the Agence Nationale pour la Recherche (ANR) and the Agence pour la Recherche sur le…
Authors not listed
This paper addresses the challenges in cell line development (CLD), the lengthy and ambiguous clone screening in upstream biopharmaceutical production. Typically, only a small subset of the later stages of CLD data is used for manually selecting lead clones. Addressing this issue, we introduce a multivariate data…
Authors not listed
The use of hybrid models, combing mechanistic and machine learning (ML), has emerged as a promising approach, contributing to the development of Industry 4.0. This work presents a hybrid model that forecasts minibioreactor (MBR) production runs of mammalian cell culture recombinant for monoclonal antibodies (mAbs)…
Authors not listed
Blinatumomab is an FDA-approved bispecific T Cell engager(BiTE) that binds CD3 on T cells and CD19 on B cells, redirecting cytotoxic T cell activity to malignant B cells. Blinatumomab has achieved clinical success in treating B-cell acute lymphoblastic leukemia(B-ALL), however, the structural and functional…
Paweł Kisielow
Demonstration that immature CD4 + 8+ thymocytes contain T cell precursors that are subjected to positive and negative selection was the major step towards understanding how the adaptive immune system acquires the ability to distinguish foreign or abnormal (mutated or infected) self-cells from normal (healthy) cells. In…