24 papers · ranked by Valyu relevance
Chengyong Ma, Hanrui Liu, Shuo Yang, Hong Li + 2 more
'Yan Kang'] Sepsis is a life-threatening syndrome caused by anomalous host response to infection. The pathogenesis of sepsis is complex, and immune dysfunction is the central link in its occurrence and development. The sepsis immune response is not a local and transient process but a complex and continuous process…
Ichwaku Rastogi, Donghwan Jeon, Jena E. Moseman, Anusha Muralidhar + 2 more
'Hemanth K. Potluri' 'Douglas G. McNeel'] B cells have been long studied for their role and function in the humoral immune system. Apart from generating antibodies and an antibody-mediated memory response against pathogens, B cells are also capable of generating cell-mediated immunity. It has been demonstrated by…
Ken Steven Rosenthal
The immune system is always acting to protect the body from the microbial world, especially at the openings of the body but also helps to maintain homeostasis. Normal functions include self-regulation and regeneration, facilitating the maintenance of organs, skin and mucosal membranes, protecting the openings of the…
Alexander Stewart, Joseph Ng, Gillian Wallis, Vasiliki Tsioligka + 2 more
Separation of B cells into different subsets has been useful to understand their different functions in various immune scenarios. In some instances, the subsets defined by phenotypic FACS separation are relatively homogeneous and so establishing the functions associated with them is straightforward. Other subsets, such…
Gabriel J. Tobón, Jorge H. Izquierdo, Carlos A. Cañas
B lymphocytes are the effectors of humoral immunity, providing defense against pathogens through different functions including antibody production. B cells constitute approximately 15% of peripheral blood leukocytes and arise from hemopoietic stem cells in the bone marrow. It is here that their antigen receptors…
Dannielle K. Moore, Bongani Motaung, Nelita du Plessis, Ayanda N. Shabangu + 2 more
'Ayanda N. Shabangu' 'André G. Loxton' '' 'Jeffrey Chalmers'] This article describes the procedures used to isolate pure B-cell populations from whole blood using various Miltenyi magnetic-activated cell sorting (MACS) bead Isolation kits. Such populations are vital for studies investigating the functional capacity of…
Michael King Yung Chung, Lanqi Gong, Dora Lai‐Wan Kwong, Victor Ho‐Fun Lee + 4 more
'Victor Ho‐Fun Lee' 'Ann Wing‐Mui Lee' 'Xin‐Yuan Guan' 'Ngar‐Woon Kam' 'Wei Dai'] Title: Abstract Most mature B cells can be divided into four subtypes based on the expression of the surface markers IgD and CD27: IgD+CD27− naïve B cells, IgD+CD27+ unswitched memory B cells, IgD−CD27+ switched memory B cells, and…
Daniel M Morelli, Morgan Langille, Richard Zhang, Heather C Craig + 3 more
B cells have been shown to be phagocytic under some circumstances. However, the phagocytic capacity of different B cell subsets and how this is linked to Antigen (Ag) presentation or other functions has not been characterized. To address this, we developed 2 µm phagocytic Ag conjugated bead targets that target…
Xiujia Yang, Haipei Tang, Chunhong Lan, Weiting He + 6 more
The development and maturation of B lymphocytes involve intricate orchestrated processes, where dedicated gene regulations (GR) take place within specific microenvironments shaped by both extracellular matrix and neighboring cells. Despite extensive investigations aimed at deepening our comprehension of these…
Tamás Szabados, Csaba Kerepesi, Tibor Bakács
Our main purpose is to compare classical nonself-centered, two-signal theoretical models of the adaptive immune system with a novel, self-centered, one-signal model developed by our research group. Our model hypothesizes that the immune system of a fetus is capable learning the limited set of self antigens but unable…
Lisa Buchauer, Hedda Wardemann
Germinal centres are anatomically defined lymphoid organ structures that mediate B cell affinity maturation and affect the quality of humoral immune responses. Mathematical models based on differential equations or agent-based simulations have been widely used to deepen our understanding of the cellular and molecular…
Alexander J Clarke, Thomas Riffelmacher, Daniel Braas, Richard J Cornall + 1 more
Specific metabolic programs are activated by immune cells to fulfil their functional roles, which include adaptations to their microenvironment. B1 B cells are tissue-resident, innate-like B cells. They have many distinct properties, such as the capacity to self-renew and the ability to rapidly respond to a limited…
Léa Hiéronimus, François Huaux
Since their discovery nearly 40 years ago, B-1 cells have continued to challenge the boundaries between innate and adaptive immunity, as well as myeloid and lymphoid functions. This B-cell subset ensures early immunity in neonates before the development of conventional B (B-2) cells and respond to immune injuries…
Ana Martínez-Riaño, Pilar Delgado, Pilar Mendoza, Clara L. Oeste + 4 more
Successful vaccines rely on activating a functional humoral response that results from generating class-switched high affinity immunoglobulins (Igs) with a superior capacity to neutralize infection. Key to this process is the germinal center (GC) reaction, in which B cells are selected in their search for antigen and T…
Miguel Ramos Pascual
A dynamic model of non-lineal time-dependent ordinary differential equations (ODE) has been applied to the interactions of a HIV infection with the immune system cells. This model has been simplified into two compartments: lymph node and peripheral blood. The model includes CD4 T-lymphocytes in several states…
Gajendra Pratap Singh, Madhuri Jha
Immunology is the emerging research area which deals with the study of immune system in any living organism. It is modeled through various computational and mathematical models to deal with the problem facing while to boost the immune system of any organism or to fight with the infectious disease at the very initial…
Elena Agliari, Adriano Barra, Gino Del Ferraro, Francesco Guerra + 1 more
'Daniele Tantari'] As for B lymphocytes, two major explanations for anergy developed over three decades: according to "Varela theory", it stems from a proper orchestration of the whole B-repertoire, in such a way that self-reactive clones, due to intensive interactions and feed-back from other clones, display more…
Authors not listed
In many autoimmune pathologies, including Rheumatoid Arthritis (RA), only a small percentage of the total B cell population is autoreactive and sustain disease. Yet, current immunotherapy treatments often eliminate the entire B-cell population, leading to immune deficiency. We developed an approach to selectively…
Rebecca L. Schmitz, Kelsey E. Tweed, Peter Rehani, Kayvan Samimi + 9 more
New non-destructive tools are needed to reliably assess lymphocyte function for immune profiling and adoptive cell therapy. Optical metabolic imaging (OMI) is a label-free method that measures the autofluorescence intensity and lifetime of metabolic cofactors NAD(P)H and FAD to quantify metabolism at a single-cell…
Rajat Desikan, Rustom Antia, Narendra M. Dixit
The affinity of antibodies (Abs) produced in vivo for their target antigens (Ags) is typically well below the maximum affinity possible. Nearly 25 years ago, Foote and Eisen explained how an 'affinity ceiling' could arise from constraints associated with the acquisition of soluble antigen by B cells. However, recent…
Clare C. Yu, Juliana C. Wortman, Ting-Fang He, Shawn T. Solomon + 11 more
'Bob Zhang' 'Anthony Rosario' 'Ruo‐Qian Wang' 'Travis Y. Tu' 'Daniel Schmolze' 'Yuan Yuan' 'Susan E. Yost' 'Xuefei Li' 'Herbert Levine' 'Gurinder S. Atwal' 'Peter P. Lee'] The goal of immunotherapy is to enhance the ability of the immune system to kill cancer cells. Immunotherapy is more effective and, in general, the…
Authors not listed
Blinatumomab is an FDA-approved bispecific T Cell engager(BiTE) that binds CD3 on T cells and CD19 on B cells, redirecting cytotoxic T cell activity to malignant B cells. Blinatumomab has achieved clinical success in treating B-cell acute lymphoblastic leukemia(B-ALL), however, the structural and functional…
Tongjin Wu, Yen Hoon Luah, Yongqiang Luo, Howard John Womersley + 1 more
Lymphocyte biology research commonly involves purification of lymphocyte subpopulations by fluorescence-activated cell sorting (FACS) or immunomagnetic separation (IMS), both of which typically rely on antibody labeling of validated cell markers. Methods enabling label-free segregation of lymphocyte subpopulations…
Amit Patel, Rahul Sharma
The human immune system is a complex network of cells, tissues, and organs that work together to protect the body from harmful pathogens. Antibodies, also known as immunoglobulins (Ig), are small proteins that play a vital role in the immune system's defense mechanism. Among the five classes of immunoglobulins…