17 papers · ranked by Valyu relevance
Alexander Stewart, Joseph Ng, Gillian Wallis, Vasiliki Tsioligka + 2 more
Separation of B cells into different subsets has been useful to understand their different functions in various immune scenarios. In some instances, the subsets defined by phenotypic FACS separation are relatively homogeneous and so establishing the functions associated with them is straightforward. Other subsets, such…
David R. Glass, Albert G. Tsai, John Paul Oliveria, Felix J. Hartmann + 4 more
To evaluate the impact of heterogeneous B cells in health and disease, comprehensive profiling is needed at a single cell resolution. We developed a highly-multiplexed screen to quantify the co-expression of 351 surface molecules on low numbers of primary cells. We identified dozens of differentially expressed…
Marina Bakardjieva, Jan Stuchlý, Ondřej Pelák, Marjolein Wentink + 8 more
Detailed knowledge of the human B-cell development is crucial for proper interpretation of inborn errors of immunity and for malignant diseases. It is of interest to understand the kinetics of protein expression changes during the B cell development, but also to properly interpret the major and possibly alternative…
Daniel M Morelli, Morgan Langille, Richard Zhang, Heather C Craig + 3 more
B cells have been shown to be phagocytic under some circumstances. However, the phagocytic capacity of different B cell subsets and how this is linked to Antigen (Ag) presentation or other functions has not been characterized. To address this, we developed 2 µm phagocytic Ag conjugated bead targets that target…
Xiujia Yang, Haipei Tang, Chunhong Lan, Weiting He + 6 more
The development and maturation of B lymphocytes involve intricate orchestrated processes, where dedicated gene regulations (GR) take place within specific microenvironments shaped by both extracellular matrix and neighboring cells. Despite extensive investigations aimed at deepening our comprehension of these…
Alexander J Clarke, Thomas Riffelmacher, Daniel Braas, Richard J Cornall + 1 more
Specific metabolic programs are activated by immune cells to fulfil their functional roles, which include adaptations to their microenvironment. B1 B cells are tissue-resident, innate-like B cells. They have many distinct properties, such as the capacity to self-renew and the ability to rapidly respond to a limited…
Fernando Y. Maeda, Jurriaan J. H. van Haaren, David B. Langley, Daniel Christ + 2 more
B-cell receptor (BCR)-mediated antigen internalization and presentation are essential for humoral memory immune responses. Antigen encountered by B-cells is often tightly associated with the surface of pathogens and/or antigen-presenting cells. Internalization of such antigens requires myosin-mediated traction forces…
Melissa Verheijen, Sanket Rane, Claire Pearson, Andrew J. Yates + 1 more
Follicular mature (FM) and germinal centre (GC) B cells underpin humoral immunity but the dynamics of their generation and maintenance are not clearly defined. Here we exploited a fate-mapping system in mice that tracks B cells as they develop into peripheral subsets, together with a cell division fate reporter mouse…
Ana Martínez-Riaño, Pilar Delgado, Pilar Mendoza, Clara L. Oeste + 4 more
Successful vaccines rely on activating a functional humoral response that results from generating class-switched high affinity immunoglobulins (Igs) with a superior capacity to neutralize infection. Key to this process is the germinal center (GC) reaction, in which B cells are selected in their search for antigen and T…
Diogo M. Cunha, Sara Hernández-Pérez, Luqman O. Awoniyi, Alexandre F. Carisey + 2 more
The immune synapse (IS) is a cell-cell interaction platform critical in lymphocyte activation by specific antigens. Despite of B cells being able to also respond to soluble antigens, in particular the in vivo importance of the IS and surface-tethered antigen recognition has strongly emerged in the recent years. The IS…
Qingtao Hu, Yu Hong, Pan Qi, Guangqing Lu + 9 more
While it has been well-recognized that T-cell mediated adaptive cellular immunity plays important roles in cancer immune response and tumor control, the roles of B lymphocytes in tumor development and therapy have only been proposed until recently, and are still mostly controversial. To gain mechanistic insights into…
Kyung-Ho Roh, Hannah K. Wilson, Pallab Pradhan, Kevin Bai + 5 more
B cells play a major role in the adaptive immune response by producing antigen-specific antibodies against pathogens and imparting immunological memory. Following infection or vaccination, antibody-secreting B cells and memory B cells are generated in specialized regions of lymph nodes and spleens, called germinal…
Timm Amendt, Lesley Vanes, Victor L. J. Tybulewicz
B-1 cells are innate-like lymphocytes that play a critical role in homeostasis by secreting natural antibodies, typically IgM, and immunosuppressive molecules such as IL-10. However, the regulation of these processes in B-1 cells remains poorly understood. Here, we demonstrate that PD-L2, a surface receptor expressed…
Theresa Pesch, Lucia Bonati, William Kelton, Cristina Parola + 4 more
Immune cell therapies based on the integration of synthetic antigen receptors provide a powerful strategy for the treatment of diverse diseases, most notably retargeting T cells engineered to express chimeric antigen receptors (CAR) for cancer therapy. In addition to T lymphocytes, B lymphocytes may also represent…
Rebecca L. Schmitz, Kelsey E. Tweed, Peter Rehani, Kayvan Samimi + 9 more
New non-destructive tools are needed to reliably assess lymphocyte function for immune profiling and adoptive cell therapy. Optical metabolic imaging (OMI) is a label-free method that measures the autofluorescence intensity and lifetime of metabolic cofactors NAD(P)H and FAD to quantify metabolism at a single-cell…
Michael H. Zhang, Brianna L. Scotland, Yun Jiao, Emily M. Slaby + 4 more
Antigen presenting cells (APCs) have been extensively studied for treating cancers and autoimmune diseases. Dendritic cells (DCs) are potent APCs that uptake and present antigens (Ags) to activate immunity or tolerance. Despite their active use in cellular immunotherapies, DCs face several challenges that hinder…
Tongjin Wu, Yen Hoon Luah, Yongqiang Luo, Howard John Womersley + 1 more
Lymphocyte biology research commonly involves purification of lymphocyte subpopulations by fluorescence-activated cell sorting (FACS) or immunomagnetic separation (IMS), both of which typically rely on antibody labeling of validated cell markers. Methods enabling label-free segregation of lymphocyte subpopulations…