26 papers · ranked by Valyu relevance
Jaya Lakshmi Bukyya, M L. Avinash Tejasvi, Anulekha Avinash, Chanchala H. P. + 6 more
'Chanchala H. P.' 'Priyanka Talwade' 'Mohammed Malik Afroz' 'Archana Pokala' 'Praveen Kumar Neela' 'T K. Shyamilee' 'Vammi Srisha'] DNA is present in most of the cells in our body, which is unique in each and every individual, and we leave a trail of it everywhere we go. This has become an advantage for forensic…
Nathan D Olson, Jayne B Morrow
Background Quantitative polymerase chain reaction (qPCR) assays used in pathogen detection require rigorous methods development including characterizing DNA extraction products. A DNA extract characterization process is demonstrated using DNA extracted from five different cells types (two Gram-negatives: Escherichia…
Lianhua Dong, Hee-Bong Yoo, Jing Wang, Sang-Ryoul Park
Digital PCR (dPCR) as an enumeration-based quantification method is capable of quantifying the DNA copy number without the help of standards. However, it can generate false results when the PCR conditions are not optimized. A recent international comparison (CCQM P154) showed that most laboratories significantly…
Abdoelnaser M. Degoot, Wilfred Ndifon
A common approach to quantifying DNA involves repeated cycles of DNA amplification. This approach, employed by the polymerase chain reaction (PCR), produces outputs that are corrupted by amplification noise, making it challenging to accurately back-calculate the amount of input DNA. Standard mathematical solutions to…
Priyanka Valloly, Rahul Roy
Amplification-based qPCR provides accurate and sensitive nucleic acid quantification. However, the requirement of temperature cycling and real-time monitoring limits its translation to different settings. Here, we adapted isothermal Recombinase Polymerase Amplification (RPA) reaction to develop a semi-quantitative…
Da-Hye Lee, Hee-Bong Yoo, Kee-Suk Hong, Sang-Ryoul Park + 2 more
'Sangkyun Jeong' 'Inchul Yang'] The mole, the SI unit for measuring the amount of a substance, was redefined as a fixed number of entities. This definition enables straightforward quantification of substances by counting individual entities. Counting proves particularly effective for quantifying large and discrete…
Andrew D. Johnston, Jennifer Lu, Darren Korbie, Matt Trau
In fragmented DNA, PCR-based methods quantify the number of intact regions at a specific amplicon length. However, the relationship between the population of DNA fragments within a sample and the likelihood they will amplify has not been fully described. To address this, we have derived a mathematical equation that…
Martina Onofri, Simona Severini, Federica Tommolini, Massimo Lancia + 4 more
'Cristiana Gambelunghe' 'Luigi Carlini' 'Eugenia Carnevali' 'Mitchell Mark Holland'] DNA quantification is a crucial step in the STR typing workflow for human identification purposes. Given the reaction’s nature, qPCR assays may be subjected to the same stochastic effects of traditional PCR for low-input…
Carina Heydt, Jana Fassunke, Helen Künstlinger, Michaela Angelika Ihle + 7 more
'Michaela Angelika Ihle' 'Katharina König' 'Lukas Carl Heukamp' 'Hans-Ulrich Schildhaus' 'Margarete Odenthal' 'Reinhard Büttner' 'Sabine Merkelbach-Bruse' 'Monika Bradl'] Over the last years, massively parallel sequencing has rapidly evolved and has now transitioned into molecular pathology routine laboratories. It is…
Peter Gill, Øyvind Bleka, Ane Elida Fonneløp
The application of qPCR to estimate the quantity of DNA present is usually based upon a short amplicon (typically c.80bp) and a longer amplicon (typically c.200-300bp) where the latter is used to determine the amount of degradation present in a sample. The data are used to make decisions about a) whether there is…
Peter Gill, Øyvind Bleka, Ane Elida Fonneløp
The application of qPCR to estimate the quantity of DNA present is usually based upon a short amplicon (typically c.80bp) and a longer amplicon (typically c.200-300bp) where the latter is used to determine the amount of degradation present in a sample. The data are used to make decisions about a) whether there is…
Robin Kuhner, Christophe Cardone, Rafael Vieira Perrella, Rabei Mohammedi + 4 more
The sensitive detection of nucleic acids is crucial for the accurate diagnosis of infections. In this context, amplification-based methods, such as the quantitative Polymerase Chain Reaction (qPCR) are the gold standard for ultrasensitive DNA or RNA detection and quantification. However, despite its widespread use in…
Authors not listed
Large-scale de novo nucleic acid synthesis is a powerful tool enabling researchers to better understand and engineer biological systems. Fields ranging from genomics to nucleic acid therapeutics to synthetic biology make use of high-throughput experimental approaches requiring access to large pools or libraries of DNA…
Chuanbo Liu, Jin Wang
Real-time PCR, or Real-time Quantitative PCR (qPCR) is an effective approach to quantify nucleic acid samples. Given the complicated reaction system along with thermal cycles, there has been long-term confusion on accurately calculating the initial nucleic acid amounts from the fluorescence signals. Although many…
Helen Miller, Adam J. M. Wollman, Mark C. Leake
PCR= Polymerase chain reaction; a method to produce multiple copies of a DNA sequence. The DNA to be copied is heated to separate the strands, and then cooled so that short 'primer' sequences bind complementarily at both 5' ends of the DNA sequence to be copied. A DNA polymerase then extends from the primers to give…
Richard Jäger, Ugo Ricci
Modern forensic DNA quantitation assays provide information on the suitability of a DNA extract for a particular type of analysis, on the amount of sample to put into the analysis in order to yield an optimal (or best possible) result, and on the requirement for optional steps to improve the analysis. To achieve a high…
Sk. Sarif Hassan, Pabitra Pal Choudhury, Antara Sengupta, Binayak Sahu + 6 more
'Binayak Sahu' 'Rojalin Mishra' 'Devendra Kumar Yadav' 'Saswatee Panda' 'Dharamveer Pradhan' 'Shrusti Dash' 'Gourav Pradhan'] In the last few decades, the human allosomes are engrossed in an intensive attention among researchers. The allosomes are now already been sequenced and found there are about 2000 and 78 genes…
David A. Russell, Erin M. Gorden, Michelle A. Peck, Christina M. Neal + 6 more
Microarray processing, which interrogates hundreds of thousands of single nucleotide polymorphisms (SNPs) across the human genome, has recently gained traction in forensics due to its use in forensic genetic genealogy, which is based on analysis using SNPs to compare distant relatives in publicly curated databases for…
Linda Jansson, Siri Aili Fagerholm, Emelie Börkén, Arvid Hedén Gynnå + 5 more
In recent years, more sophisticated DNA technologies for genotyping have enabled considerable progress in various fields such as clinical genetics, archaeogenetics and forensic genetics. DNA samples previously rejected as too challenging to analyze due to low amounts of degraded DNA can now provide useful information.…
Authors not listed
Stretches of double-stranded DNA sharing the same sequence can recognise each other in cells. This phenomenon, known as homologous recognition, is essential for DNA recombination and repair. Yet, its mechanism remains debated, with purely physical duplex duplex interactions being proposed as a contributing factor.…
Xiaoting Xue, Helena Persson, Lei Ye
Accurate and rapid detection of nucleic acid sequences is of utmost importance in various fields, including disease monitoring, clinical treatment, gene analysis and drug discovery. In this study, we developed a "turn-on" fluorescence biosensor that enables simple and highly efficient detection of nucleic acid…
James E. Barrett, Andrew Feber, Javier Herrero, Miljana Tanić + 3 more
'Gareth A. Wilson' 'Charles Swanton' 'Stephan Beck'] Motivation: Epigenetic heterogeneity within a tumour can play an important role in tumour evolution and the emergence of resistance to treatment. It is increasingly recognised that the study of DNA methylation (DNAm) patterns along the genome – so-called 'epialleles'…
Man Zhang, Lei Ye
Using rolling circle amplification (RCA) and two different ways of signal readout, we developed analytical methods to detect the receptor binding domain (RBD) of SARS-CoV 2 spike protein (S protein). We modified streptavidin-coated magnetic beads with an aptamer of RBD through a biotin-tagged complementary DNA strand…
Nasir Uddin, Md Abu Monsur Dinar, Daniel Pack, Jason DeRouchey
Polyethylenimine (PEI), a widely used cationic polymer for nonviral gene delivery, often undergoes modification to enhance its transfection efficiency while reducing cytotoxicity. This study systematically examines the effects of acetylation and succinylation on PEI’s physicochemical properties, transfection…
Authors not listed
Oligonucleotides offer a powerful class of new therapeutic modalities, which requires the support of robust and sensitive analytical methods. The primary structure of RNA-based therapeutic drugs is considered a critical quality attribute by regulatory agencies, and must be empirically confirmed to ensure quality…
HANIYEH ABDOLLAHZADEH, Tonya Peeples, Mohammad Shahcheraghi
DNA-based nanomaterials have shown great potential in numerous applications, thanks to their unique properties including DNA's various molecular interactions, programmability, and versatility with biological modules. Meanwhile, the DNA origami platforms have shown promise in the creation of drug carriers. This…