27 papers · ranked by Valyu relevance
Yong Wang, Xiushuo Zhang, Jing Xu, Xiangyu Sun + 9 more
'Hongsheng Li' 'Yanping Liu' 'Jingjing Tian' 'Xiaorui Hao' 'Xiaofei Kong' 'Zhiwei Wang' 'Jie Yang' 'Yuqing Su'] As a typical microscopic imaging technology, the emergence of the microscope has accelerated the pace of human exploration of the micro world. With the development of science and technology, microscopes have…
Matthew Wincott, Andrew Jefferson, Ian M. Dobbie, Martin J. Booth + 2 more
Commercial fluorescence microscope stands and fully automated XYZt fluorescence imaging systems are generally beyond the limited budgets available for teaching and outreach. We have addressed this problem by developing “Microscopi”, an accessible, affordable, DIY automated imaging system that is built from 3D printed…
Ethan Li, Vittorio Saggiomo, Wei Ouyang, Manu Prakash + 1 more
Microscopy is essential for detecting, identifying, analyzing, and measuring small objects. Access to modern microscopy equipment is crucial for scientific research, especially in the biomedical and analytical sciences. However, the high cost of equipment, limited availability of parts, and challenges associated with…
Kleoniki Keklikoglou, Christos Arvanitidis, Georgios Chatzigeorgiou, Eva Chatzinikolaou + 13 more
'Eva Chatzinikolaou' 'Efstratios Karagiannidis' 'Triantafyllia Koletsa' 'Antonios Magoulas' 'Konstantinos Makris' 'George Mavrothalassitis' 'Eleni-Dimitra Papanagnou' 'Andreas S. Papazoglou' 'Christina Pavloudi' 'Ioannis P. Trougakos' 'Katerina Vasileiadou' 'Angeliki Vogiatzi' 'Maria Pia Morigi' 'Fauzia Albertin']…
Ekta Prajapati, Saurav Kumar, Shishir Kumar
In the past few decades, a significant amount of effort has been put into developing different lensless microscope designs. The existing lensless microscopes are capable of offering high resolution and wide field-of-view using super-resolution and computational techniques. But, the employment of macroscopic…
S. Weisenburger, Vahid Sandoghdar
Optical microscopy is one of the oldest scientific instruments that is still used in forefront research. Ernst Abbe's nineteenth century formulation of the resolution limit in microscopy let generations of scientists believe that optical studies of individual molecules and resolving sub-wavelength structures were not…
Richard W. Taylor, Vahid Sandoghdar
Interferometric scattering (iSCAT) microscopy is a powerful tool for label-free sensitive detection and imaging of nanoparticles to high spatio-temporal resolution. As it was born out of detection principles central to conventional microscopy, we begin by surveying the historical development of the microscope to…
Matthew Wincott, Andrew Jefferson, Ian M. Dobbie, Martin J. Booth + 2 more
Commercial fluorescence microscope stands and fully automated XYZt fluorescence imaging systems are generally beyond the limited budgets available for teaching and outreach. We have addressed this problem by developing “Microscopi”, an accessible, affordable, DIY automated imaging system that is built from 3D printed…
Samuel B. Tristan-Landin, Alan M. Gonzalez-Suarez, Rocio J. Jimenez-Valdes, Jose L. Garcia-Cordero
Fluorescence microscopy is one of the workhorses of biomedical research and laboratory diagnosis; however, their cost, size, maintenance, and fragility has prevented their adoption in developing countries or low-resource settings. Although significant advances have decreased their size, cost and accessibility, their…
Adam J. M. Wollman, Richard Nudd, Erik Hedlund, Mark C. Leake
Although not laying claim to being the inventor of the light microscope, Antonj van Leeuwenhoek, (1632 –1723) was arguably the first person to bring this new technological wonder of the age properly to the attention of natural scientists interested in the study of living things (people we might now term 'biologists').…
Guillermo J. Tearney
Today's gold standard for medical diagnosis is histology of excised biopsies or surgical specimens where tissue is taken out of the body, processed, sectioned, stained, and looked at under a light microscope by a pathologist. There are many limitations of this technique, including the fact that it is inherently…
Samuel B. Tristan-Landin, Alan M. Gonzalez-Suarez, Rocio J. Jimenez-Valdes, Jose L. Garcia-Cordero + 1 more
'Rocio J. Jimenez-Valdes' 'Jose L. Garcia-Cordero' 'Virgilio Mattoli'] Fluorescence microscopy is one of the workhorses of biomedical research and laboratory diagnosis; however, their cost, size, maintenance, and fragility has prevented their adoption in developing countries or low-resource settings. Although…
Egidijus Auksorius
This thesis centres on the development of multidimensional fluorescence imaging tools, with a particular emphasis on fluorescence lifetime imaging (FLIM) microscopy for application to biological research. The key aspects of this thesis are the development and application of tunable supercontinuum excitation sources…
S. Foylan, W. B. Amos, J. Dempster, L. Kölln + 3 more
Total Internal Reflection Fluorescence (TIRF) illumination bypasses the axial diffraction limit of light by using an evanescent field to excite fluorophores close to a sample substrate. TIRF illumination significantly improves image contrast, allowing researchers to study membrane structure and dynamics with localized…
Loïs Mignard-Debise, Ivo Ihrke
We explore the use of inexpensive consumer lightfield camera technology for the purpose of light-field microscopy. Our experiments are based on the Lytro (first generation) camera. Unfortunately, the optical systems of the Lytro and those of microscopes are not compatible, leading to a loss of light-field information…
Rachel Kemp Alexander Chippendale, Monica Harrelson, S. E. Zuraw, Amanda Tan + 2 more
'Amanda Tan' 'Jennifer L. Ross' 'Jennifer Shumway'] Optics is an important subfield of physics required for instrument design and used in a variety of other disciplines, including materials science, physics, and life sciences such as developmental biology and cell biology. It is important to educate students from a…
Zengbo Wang
Recently, it was discovered that microsphere can generate super-resolution focusing beyond diffraction limit. This has led to the development of an exciting super-resolution imaging technique -microsphere nanoscopy- that features a record resolution of 50 nm under white lights. Different samples have been directly…
Chengzhuang Yu, Shanshan Li, Chunyang Wei, Shijie Dai + 5 more
'Junwei Li' 'Bobby Mathew' 'Nien-Tsu Huang' 'Sherif M. Karam'] A fluorescence microscope is one of the most important tools for biomedical research and laboratory diagnosis. However, its high cost and bulky size hinder the application of laboratory microscopes in space-limited and low-resource applications. Here, in…
Wenbo Jiang, Jingchun Wang, Yidi Yang, Yun Bu + 1 more
Conventional optical microscopes are only able to resolve objects down to a size of approximately 200 nm due to optical diffraction limits. The rapid development of nanotechnology has increased the demand for greater imaging resolution, with a need to break through those diffraction limits. Among super-resolution…
Hongli Jiang, S. Hessam M. Mehr
The availability, portability, and low cost of electronic devices have made them a prime candidate for the rapid detection of chemical particles. Here we designed a chemical particle detection system based on a Raspberry Pi camera to detect micron droplets generated by ultrasonic atomizers. Through the analysis of…
Michael Andrews, Debbie Jones, Adam Woodward, Adam Swinburne + 5 more
Nuclear power is a sustainable zero-carbon energy source but requires generated radionuclide waste be remediated from contaminated lands. Legacy, mining and disposal activities all engender environmental contamination, particularly for uranium, which is the main component by mass being both radioactive and toxic.…
Kanda Borgognoni, Bradley Guilliams, Rachel Cohen, Christopher Ackerson
A cloneable NanoParticle (cNP) is an inorganic nanoparticle that is synthesized by a protein. The protein determines the elemental composition, size, morphology, and other properties of the nanoparticle. Here, we describe the use of a cloneable Selenium NanoParticle (cSeNP) as a cloneable imaging contrast agent in…
Tobias A. Dancker, Mohamed Ibrahem Elhawy, Ramona Rittershauß, Qinghai Tian + 13 more
Microendoscopy enables minimally invasive investigations of organs even within small cavities. Conventional microendoscopy is limited by probe size and often restricted to a single excitation wavelength. We developed and characterized a multichannel microendoscope as thin as 360 µm and recorded functional cellular…
Abbas Jabermoradi, John van Duynhoven, Johannes Hohlbein
Emulsifiers play an essential role in ensuring the physiochemical stability of food emulsions. In the case of mayonnaise, proteins contained in egg yolk act as emulsifiers. Here, we employed stochastic optical reconstruction microscopy (STORM) to localize proteins at the oil/water droplet interface using fluorescently…
M. Marini, A. Nardini, R. Martínez Vázquez, C. Conci + 9 more
Non-linear excitation microscopy offers several advantages for in-vivo imaging compared to conventional confocal techniques. However, tissue penetration can still be an issue due to scattering and spherical aberrations induced on focused beams by the tissue. The use of low numerical aperture objectives to pass through…
Suyeon Yang, Machi Takeuchi, Rick Joosten, John van Duynhoven + 2 more
Lipid oxidation is a major cause of product deterioration in protein stabilised oil-in-water food emulsions. The impact of protein emulsifiers on lipid oxidation and the stability depends on the specific type of protein emulsifiers used and the redox conditions in the emulsion. However, the exact impact of these…
Domingo Olivares Postigo, Federico Gorrini, Valeria Bitonto, Johannes Ackermann + 3 more
Ensembles of negatively charged nitrogen vacancy centers (NV-) in diamond have been proposed for sensing of magnetic fields and paramagnetic agents, and as a source of spin-order for the hyperpolarization of nuclei in magnetic resonance applications. To this end, strongly fluorescent nanodiamonds represent promising…