21 papers · ranked by Valyu relevance
Srinivas S. Somanchi, Kelsey J. McCulley, Anitha Somanchi, Leo L. Chan + 2 more
'Leo L. Chan' 'Dean A. Lee' 'R. Keith Reeves'] Natural killer (NK) cells belong to the innate arm of the immune system and though activated NK cells can modulate immune responses through the secretion of cytokines, their primary effector function is through target cell lysis. Accordingly, cytotoxicity assays are…
Snehal Shabrish, Maya Gupta, Manisha Madkaikar
Natural killer (NK) cells play important role in innate immunity against tumors and viral infections. Studies show that lysosome-associated membrane protein-1 (LAMP-1, CD107a) is a marker for degranulation of NK and cytotoxic T cells and its expression is a sensitive marker for the cytotoxic activity determination. The…
Jinho Kim, Minh-Trang Thi Phan, SoonHo Kweon, HongBi Yu + 6 more
'Jeehun Park' 'Kyeong-Hee Kim' 'Ilwoong Hwang' 'Sangbin Han' 'Min-Jung Kwon' 'Duck Cho'] Background: Measurement of natural killer (NK) cell function has important clinical utility in several diseases. Although the flow cytometry (FC)-based 4-h NK cytotoxicity assay using peripheral blood mononuclear cells (PBMCs) in…
Olivier Morteau, Samkeliso Blundell, Aron Chakera, Sophia Bennett + 5 more
'Charita M. Christou' 'Philip D. Mason' 'Richard J. Cornall' "Christopher A. O'Callaghan" 'Samithamby Jeyaseelan'] Background Despite an increasing awareness of the importance of innate immunity, the roles of natural killer (NK) cells in transplant rejection and antiviral and cancer immunity during immunosuppression…
Lyndsey Feehan, Leslie Koutoufaris, Jeri Dorsey, Michele Paessler + 1 more
Natural killer (NK) cell degranulation is a key immune defense mechanism where exposure to tumor or virus-infected cells triggers the fusion of cytoplasmic granules containing apoptotic proteins, perforin, and granzyme with the cell membrane. This process transiently expresses CD107a on the NK cell surface, and…
Ki-Hyun Park, Hyesun Park, Myungshin Kim, Yonggoo Kim + 2 more
'Eun-Jee Oh'] Although real-time cell electronic sensing (RT-CES) system-based natural killer (NK) cytotoxicity has been introduced, it has not been evaluated using human blood samples. In present study, we measured flowcytometry based assay (FCA) and RT-CES based NK cytotoxicity and analyzed degranulation activity…
Valeria Vasilyeva, Olivia Makinson, Cynthia Chan, Maria Park + 24 more
Natural Killer (NK) cells are critical for immunosurveillance yet become dysfunctional in contexts such as chronic stimulation by viral infections or cancer. This phenomenon is similar to T cell exhaustion but less well characterized, which limits therapeutic interventions. As shown for T cells, NK cells often display…
Mercede Lee, Charles J M Bell, Arcadio Rubio Garcia, Leila Godfrey + 4 more
'Marcin Pekalski' 'Linda S Wicker' 'John A Todd' 'Ricardo C Ferreira'] Title: Abstract Human CD56br natural killer (NK) cells represent a small subset of CD56+ NK cells in circulation and are largely tissue-resident. The frequency and number of CD56br NK cells in blood has been shown to increase following…
Audrey Guilbaud, Baptiste Lottin, Gwenann Cadiou, Tiffany Beauvais + 5 more
Natural killer (NK) cells play a crucial role in the innate immune response to bacterial infections, including those due to Pseudomonas aeruginosa (P. aeruginosa) and Staphylococcus aureus (S. aureus). In vivo, it has been shown that NK cells are activated by innate accessory cells that detect the presence of bacteria…
Jacob Hanna, Ofer Mandelboim
Initiation of the adaptive immune response is dependent on the priming of naive T cells by APCs. Proteomic analysis of unactivated and activated human NK cell membrane–enriched fractions demonstrated that activated NK cells can efficiently stimulate T cells, since they upregulate MHC class II molecules and multiple…
Katja Hellendahl, Maryke Fehlau, Sebastian Hans, Peter Neubauer + 1 more
Nucleoside kinases (NKs) are key enzymes involved in the in vivo phosphorylation of nucleoside analogues used as drugs to treat cancer or viral infections. Having different specificities, the characterization of NKs is essential for drug design and the production of nucleotide analogues in an in vitro enzymatic…
Christoph Mark, Tina Czerwinski, Susanne Roessner, Astrid Mainka + 9 more
Natural killer (NK) cells are important effector cells in the immune response to cancer. Clinical trials on adoptively transferred NK cells in patients with solid tumors, however, have thus far been unsuccessful. As NK cells need to pass stringent safety evaluation for clinical use, the cells are cryopreserved to…
Sophie V. Pageon, Gerardo Aquino, Kathryn Lagrue, Karsten Köhler + 2 more
'Robert G. Endres' 'Daniel M. Davis'] Natural Killer (NK) cell activation is dynamically regulated by numerous activating and inhibitory surface receptors that accumulate at the immune synapse. Quantitative analysis of receptor dynamics has been limited by methodologies which rely on indirect measurements such as…
Arthur Lui, Ju‐Hee Lee, Peter F. Thall, May Daher + 2 more
'Rafet Barar'] A Bayesian feature allocation model (FAM) is presented for identifying cell subpopulations based on multiple samples of cell surface or intracellular marker expression level data obtained by cytometry by time of flight (CyTOF). Cell subpopulations are characterized by differences in expression patterns…
Vasiliki Bitsouni, Vasilis Tsilidis
tBregs are a newly discovered subcategory of B regulatory cells, which are generated by breast cancer, resulting in the increase of Tregs and therefore in the death of NK cells. In this study, we use a mathematical and computational approach to investigate the complex interactions between the aforementioned cells as…
Claus Metzner, F Horsch, Christoph Mark, Tina Czerwinski + 3 more
'Alexander Winterl' 'Caroline Voskens' 'Ben Fabry'] Chemotaxis enables cells to systematically approach distant targets that emit a diffusible guiding substance. However, the visual observation of an encounter between a cell and a target does not necessarily indicate the presence of a chemotactic approach mechanism, as…
Authors not listed
Hodgkin’s lymphoma is a malignancy (cancer) that affects the immune system, primarily lymphocytes (white blood cells). CD30 is a protein receptor expressed on the surface of Hodgkin's cancer cells and is a crucial target for diagnosis and treatment. As of now, current CD30-targeted therapies come with extensive side…
Authors not listed
The ability to track therapeutic cells is critical for advancing adoptive cell therapy (ACT). Positron emission tomography (PET) offers sensitive and quantitative imaging, yet improved cell radiolabeling strategies are sorely needed. We report a metabolic glycoengineering (MGE) approach that installs azide moieties on…
Jonas Wallin, Kerstin Johnsson, Magnus Fontes
Flow cytometry is a widespread single-cell measurement technology with a multitude of clinical and research applications. Interpretation of flow cytometry data is hard; the instrumentation is delicate and can not render absolute measurements, hence samples can only be interpreted in relation to each other while at the…
Kavita Iyer, Julian Ivanov, Rumiana Tenchov, Krittika Ralhan + 4 more
Rapid and sustained growth in the field of immuno-oncology has resulted in expansion of available scientific literature. Gaining valuable insights and establishing deep and often hidden meaningful connections in such a large body of work is the need of the hour. In this report we summarize our findings from a novel…
liu wenzhong, li hualan
The high mortality of COVID-19 is related to poor antigen presentation and lymphopenia. In this present study, domain search results showed that many proteins of the SARS-COV-2 virus had MHC-like domains, which were similar to decoys for the human immune system. MHC-like structures could bind to MHC receptors of immune…