26 papers · ranked by Valyu relevance
Ziyi Zhao, Yong Mei, Ziyang Wang, Weiling He + 1 more
Simple Summary Drug therapy is an important treatment for cancer patients; however, drug resistance severely affects the survival time and quality of life of cancer patients. Oxidative phosphorylation (OXHPOS) is an important metabolic process in cells that drives cancer drug resistance and exerts a significant…
Géraldine Gentric, Yann Kieffer, Virginie Mieulet, Oumou Goundiam + 14 more
Title: Summary High-grade serous ovarian cancer (HGSOC) remains an unmet medical challenge. Here, we unravel an unanticipated metabolic heterogeneity in HGSOC. By combining proteomic, metabolomic, and bioergenetic analyses, we identify two molecular subgroups, low- and high-OXPHOS. While low-OXPHOS exhibit a glycolytic…
Jean-Claude Farré, Krypton Carolino, Lou Devanneaux, Suresh Subramani
How environmental cues influence peroxisome proliferation, particularly through other organelles, remains largely unknown. Yeast peroxisomes metabolize all fatty acids (FA), and methylotrophic yeasts also metabolize methanol. NADH and acetyl-CoA, the products of these pathways enter mitochondria for ATP production, and…
Ilya N Boykov, McLane M Montgomery, James T Hagen, Raphael T Aruleba + 10 more
Targeting mitochondrial oxidative phosphorylation (OXPHOS) to combat cancer is increasingly being investigated using a variety of small molecule inhibitors. Clinical success for these inhibitors has been hampered due to serious side-effects potentially arising from the inability to discriminate between non-cancerous…
Andreas Carlström, Carmela Vazquez‐Calvo, Martin Ott
Mitochondrial oxidative phosphorylation is the most efficient way of energy conversion for eukaryotic cells. It is executed by a series of high-molecular weight enzyme complexes in the inner mitochondrial membrane that were acquired during endosymbiosis at the root of eukaryotic evolution. Biogenesis of this machinery…
Jia Xin Tang, Kyle Thompson, Robert W. Taylor, Monika Oláhová
The assembly of mitochondrial oxidative phosphorylation (OXPHOS) complexes is an intricate process, which-given their dual-genetic control-requires tight co-regulation of two evolutionarily distinct gene expression machineries. Moreover, fine-tuning protein synthesis to the nascent assembly of OXPHOS complexes requires…
Gilad Barshad, Amit Blumberg, Dan Mishmar
In humans, oxidative phosphorylation (OXPHOS), the cellular energy producer, harbors ∼90 nuclear DNA (nDNA)- and mitochondrial DNA (mtDNA)-encoded subunits. Although nDNA- and mtDNA-encoded OXPHOS proteins physically interact, their transcriptional regulation profoundly diverges, thus questioning their co-regulation.…
Guillermo Puertas-Frias, María José-Saucedo-Rodríguez, Kristýna Čunátová, Lukáš Alán + 16 more
Impaired energy production is a hallmark of mitochondrial oxidative phosphorylation (OXPHOS) defects. However, secondary metabolic disturbances also represent an important trigger for pathologies originating from OXPHOS aberrations. We have discovered that cells with OXPHOS deficiencies accumulate triacylglycerols…
Rudan Xiao, Lijuan Zhang, Zijuan Xin, Junwei Zhu + 10 more
Diamond-Blackfan anemia (DBA) is a rare congenital disorder characterized by the failure of erythroid progenitor differentiation; however, the molecular mechanisms leading to erythroid defects remain unclear. By analyzing the transcriptomic profiles of bone marrow from patients with DBA (n = 10), we identified the…
Abena Nsiah-Sefaa, Matthew McKenzie
Mitochondria provide the main source of energy to eukaryotic cells, oxidizing fats and sugars to generate ATP. Mitochondrial fatty acid β-oxidation (FAO) and oxidative phosphorylation (OXPHOS) are two metabolic pathways which are central to this process. Defects in these pathways can result in diseases of the brain…
Tchern Lenn, Mark C. Leake
Although significant insight has been gained into biochemical, genetic and structural features of oxidative phosphorylation (OXPHOS) at the single-enzyme level, relatively little was known of how the component complexes function together in time and space until recently. Several pioneering single-molecule studies have…
André F. Camargo, Sina Saari, Geovana S. Garcia, Marina M. Chioda + 8 more
Despite the beneficial effects shown when the mitochondrial alternative oxidase AOX from Ciona intestinalis (Tunicata: Ascidiacea) is xenotopically expressed in mammalian and insect models, important detrimental outcomes have also been reported, raising concerns regarding its envisioned deployment as a therapy enzyme…
Kelath Murali Manoj
The prevailing half-a-century old explanation for cellular respiration solicits a synchronization of electron transfer chain (ETC) and proton pumping activity across the inner mitochondrial membrane, for generating a trans-membrane proton-electro-chemical potential. This outcome is supposedly harnessed by Complex V to…
Kelath Murali Manoj
Via a concurrently communicated manuscript [Manoj, 2017], I have conclusively debunked the long-standing "Electron Transport Chain – Proton Pump – Chemiosmosis – Rotary ATP Synthesis" (EPCR) explanatory paradigm for mitochondrial oxidative phosphporylation (mOxPhos). Now, the mandate is open to establish a viable…
Alexei Vázquez
The utilization of glycolysis in aerobic conditions have been a subject of debate for more than a century. A hypothesis supported by previous data is that glycolysis has a higher rate of ATP production per protein mass and per occupied volume than oxidative phosphorylation (OxPhos). However, a recent work by Shen et…
Ilka Starke, Gary D. Glick, Michael Börsch
Targeting the mitochondrial enzyme FoF1-ATP synthase and modulating its catalytic activities with small molecules is a promising new approach for treatment of autoimmune diseases. The immunomodulatory compound Bz-423 is such a drug that binds to subunit OSCP of the mitochondrial FoF1-ATP synthase and induces apoptosis…
Authors not listed
EOxidase-based electrochemical biosensors are widely deployed for point-of-use applications, yet oxygen interference remains a major challenge, significantly reducing accuracy at low concentrations. Here, we developed a universal enzymatic O2 scavenger composed of alcohol oxidase, catalase, and paraformaldehyde to…
Jayamini Jayawardhane, Devin W. Cochrane, Poorva Vyas, Natalia V. Bykova + 2 more
'Natalia V. Bykova' 'Greg C. Vanlerberghe' 'Abir U. Igamberdiev'] Alternative oxidase (AOX) is a non-energy conserving terminal oxidase in the plant mitochondrial electron transport chain (ETC) that has a lower affinity for oxygen than does cytochrome (cyt) oxidase. To investigate the role(s) of AOX under different…
Léa El Khoury, David Mobley, Dongmei Ye, Susan Rempe
Organophosphorus (OP) compounds are among the most toxic of chemical substances and widely used as insecticides, pesticides, and chemical warfare agents. The most important enzyme inhibited by OP compounds is acetylcholinesterase (AChe). Inactivation of AChe function results in the accumulation of neurotransmitter…
Gabriela C. Schröder, William B. O'Dell, Simon P. Webb, Pratul K. Agarwal + 1 more
Metalloproteins perform a diverse array of redox-related reactions facilitated by the increased chemical functionality afforded by their metallocofactors. Lytic polysaccharide monooxygenases (LPMOs) are a class of copper-dependent enzymes that are responsible for the breakdown of recalcitrant polysaccharides via…
Lea Eilert, Anett Schallmey, Felix Kaspar
Despite the prevalence of ortho- and pyrophosphate in biochemistry, operationally simple and versatile high-throughput methodologies for their quantification are lacking. We herein introduce PUB, a module for phosphate detection by continuous UV-spectroscopic monitoring of 5-bromouridine phosphorolysis. The PUB module…
Marten Szibor, Christina Schenkl, Mario R. O. Barsottini, Luke Young + 1 more
'Anthony L. Moore'] Some of the most threatening human diseases are due to a blockage of the mitochondrial electron transport chain (ETC). In a variety of plants, fungi, and prokaryotes, there is a naturally evolved mechanism for such threats to viability, namely a bypassing of the blocked portion of the ETC by…
Giorgia Maroni, Eva Cabrera San Millan, Beatrice Campanella, Massimo Onor + 8 more
- (1) CNR-Consiglio Nazionale delle Ricerche, Istituto di Tecnologie Biomediche (ITB-CNR), SS di Pisa, Area della Ricerca di Pisa, Italy - (2) CNR-Consiglio Nazionale delle Ricerche, Istituto di Chimica dei Composti OrganoMetallici (ICCOM-CNR), SS di Pisa, Area della Ricerca di Pisa, Italy - (3) Scuola Normale di Pisa…
Ana De La Fuente Durán, Allen Yu-Lun Liang, Ilaria Denti, Hang Yu + 13 more
Ana De La Fuente Durán 1 , Allen Yu-Lun Liang 1 , Ilaria Denti 1 , Hang Yu 2 , Drew Pearce 2 , Adam Marks 1 , Emily Penn 1 , Karrie Weaver 1 , Lily Turaski 1 , Iuliana P. Maria 3,2 , Sophie Griggs 3 , Xingxing Chen 4 , Alberto Salleo 1 , William C. Chueh 1 , Jenny Nelson , Alexander Giovannitti 1 , J. Tyler Mefford 1
Regine Siedentop, Tobias Prenzel, Siegfried R. Waldvogel, Katrin Rosenthal + 1 more
'Katrin Rosenthal' 'Stephan Lütz'] Adenosine-5'-triphosphate (ATP) plays a crucial role in many biocatalytic reactions and its regeneration can influence the performance of a related enzymatic reaction significantly. Here, we established an electrochemically coupled ATP regeneration by pyruvate oxidase and acetate…
Jose Lanuza, Veronica Postils, Xabier Lopez
The increased bioavailability of aluminum has led to a concern about its toxicity on living systems. Among the most important toxic effects, it has been proven that aluminum increases oxidative stress in biological systems, a controversial fact, however, due to its non-redox nature. In the present work, we characterize…