23 papers · ranked by Valyu relevance
Wen Deng, Weiguo Zheng, Hong Cheng
As a fundamental task in graph data management, maximal clique enumeration (MCE) has attracted extensive attention from both academic and industrial communities due to its wide range of applications. However, MCE is very challenging as the number of maximal cliques may grow exponentially with the number of vertices.…
Thomas Rose, Annette Knabe, Rita Berthold, Kristin Höwing + 3 more
In early development stages of biologicals there is often more than one molecule against a specific target. A careful candidate evaluation is crucial to choose an optimal lead variant for further development. Complex biologicals are typically produced in CHO cells and host cells as well as the process are known to…
Michael Nonet, Christian Frøkjær-Jensen
Recombination-mediated cassette exchange (RMCE) is a recently developed alternative method for creating single copy transgenes using recombination rather than repair of double stranded breaks as the mechanism for driving integration into the genome. Two alternative methods for performing RMCE have been developed; a…
Morten M. Callesen, Martin F. Berthelsen, Sira Lund, Annette C. Füchtbauer + 3 more
'Annette C. Füchtbauer' 'Ernst-Martin Füchtbauer' 'Jannik E. Jakobsen' 'Peter Hohenstein'] Recombinase mediated cassette exchange (RMCE) is a powerful tool for targeted insertion of transgenes. Here we describe non-proprietary 'RMCE-in' cell lines as an alternative to the 'Flp-in' system and cell lines. RMCE-in cell…
Michael L. Nonet
I outline a streamlined method to create single copy large genomic insert transgenes using Recombination-Mediated Cassette Exchange (RMCE) that relies solely on drug selection yielding a homozygous fluorescent protein (FP) marked transgene in 3 generations (8 days) at high efficiency (>1 insertion per 2 injected P0…
Fang-Jung Yang, Chiao-Nung Chen, Tiffany Chang, Ting-Wei Cheng + 6 more
C. elegans benefits from a large set of tools for genome manipulation. Yet, the insertion of large DNA constructs and the generation of inversions is still challenging. Here, we adapted the phiC31 integrase system for C. elegans. We generated an integrated phiC31 integrase expressing strain flanked by attP sites that…
Guillaume Urtecho, Arielle D. Tripp, Kimberly D. Insigne, Hwangbeom Kim + 1 more
Promoters are the key drivers of gene expression and are largely responsible for the regulation of cellular responses to time and environment. In E. coli, decades of studies have revealed most, if not all, of the sequence elements necessary to encode promoter function. Despite our knowledge of these motifs, it is still…
Anzar Ali, Shama Shama, Yogesh Singh
The rotating magnetocaloric effect (RMCE) is a recent interest in magnetic refrigeration technique in which the cooling effect is attained by rotating the anisotropic magnetocaloric material from one orientation to the other in a fixed magnetic field. In this work, we report the anisotropic magnetocaloric properties of…
Jannik Ejnar Jakobsen, Marianne G. Johansen, Mette Schmidt, Frederik Dagnæs-Hansen + 10 more
'Frederik Dagnæs-Hansen' 'Karen Dam' 'Anders Gunnarsson' 'Ying Liu' 'Peter M. Kragh' 'Rong Li' 'Ida E. Holm' 'Henrik Callesen' 'Jacob Giehm Mikkelsen' 'Anders Lade Nielsen' 'Arne Lund Jørgensen'] Targeted transgenesis using site-specific recombinases is an attractive method to create genetically modified animals as it…
Daniel Mariyappa, Arthur Luhur, Danielle Overton, Andrew C. Zelhof
The generation of Drosophila stable cell lines have become invaluable for complementing in vivo experiments and as tools for genetic screens. Recent advances utilizing attP/PhiC31 integrase system has permitted the creation of Drosophila cells in which recombination mediated cassette exchange (RMCE) can be utilized to…
Patrick Mayrhofer, Bernhard Kratzer, Wolfgang Sommeregger, Willibald Steinfellner + 6 more
'Willibald Steinfellner' 'David Reinhart' 'Alexander Mader' 'Soeren Turan' 'Junhua Qiao' 'Juergen Bode' 'Renate Kunert'] Over the years, Chinese hamster ovary (CHO) cells have emerged as the major host for expressing biotherapeutic proteins. Traditional methods to generate high-producer cell lines rely on random…
Benjamin E. Low, Vishnu Hosur, Simon Lesbirel, Michael V. Wiles
Efficient, targeted integration of large DNA constructs represent a significant hurdle in genetic engineering for the development of mouse models of human disease and synthetic biology research. To address this, we developed a system for efficient and precise, targeted single-copy integration of large transgenes…
Brigitte Boden, Jan Flink, Niklas Först, Robert Mischke + 4 more
'Kathrin Schaffert' 'Alexander Weinert' 'Annika Wohlan' 'Andreas Schreiber'] Abstract. Engineering of complex systems such as air- and spacecraft is a multidisciplinary effort that requires the collaboration of engineers from a multitude of specializations working in concert. Typically, each engineer uses one or more…
Mengjie Liu, Alon Grinberg Dana, Matthew Johnson, Mark Goldman + 10 more
In chemical kinetics research, kinetic models containing hundreds of species and tens of thousands of elementary reactions are commonly used to understand and predict the behavior of reactive chemical systems. Reaction Mechanism Generator (RMG) is a software suite developed to automatically generate such models by…
Authors not listed
Organometallic catalysis lies at the heart of numerous industrial processes that produce bulk and fine chemicals. The search for transition states and screening for organic ligands are vital in designing highly active organometallic catalysts with efficient reaction kinetics. However, identifying accurate transition…
Xuejing Zheng, Chao Yu
In this paper, we study the cooperative Multi-Agent Reinforcement Learning (MARL) problems using Reward Machines (RMs) to specify the reward functions such that the prior knowledge of high-level events in a task can be leveraged to facilitate the learning efficiency. Unlike the existing work that RMs have been…
Tabia Henry Akintobi, Payam Sheikhattari, Emma Shaffer, Christina L. Evans + 24 more
This paper details U.S. Research Centers in Minority Institutions (RCMI) Community Engagement Cores (CECs): (1) unique and cross-cutting components, focus areas, specific aims, and target populations; and (2) approaches utilized to build or sustain trust towards community participation in research. A mixed-method data…
Daniel F. Sarpong, Corrine Yibing Liu, Tandeca King Gordon, Angela Sy + 3 more
As of the end of 2024, the NIH-funded Research Centers in Minority Institutions (RCMI) Program supported 21 specialized centers at minority-serving institutions. Its goal is to strengthen biomedical research infrastructure and enhance the capacity of investigators focused on health disparities. Since 2017, the centers…
Nick Brown, Michael Bareford, Michèle Weiland
Remote Memory Access (RMA), also known as single sided communications, provides a way of accessing the memory of other processes without having to issue explicit message passing style communication calls. Previous studies have concluded that MPI RMA can provide increased performance over traditional MPI Point to Point…
Emily Mazeau, Priyanka Satpute, Katrin Blondal, Franklin Goldsmith + 1 more
Kinetic parameters for surface reactions can be predicted using a combination of DFT calculations, scaling relations, and machine learning algorithms; however, construction of microkinetic models still requires a knowledge of all the possible, or at least reasonable, reaction pathways. The recently developed Reaction…
Zanetta Chang, Joseph Heitman
The environmentally ubiquitous fungus Mucor circinelloides is a primary cause of the emerging disease mucormycosis. Mucor infection is notable for causing high morbidity and mortality, especially in immunosuppressed patients, while being inherently resistant to the majority of clinically available antifungal drugs. A…
Authors not listed
The consideration of transition state (TS) conformer ensembles is required to accurately model a reaction, and thus plays a key role in computational catalyst design. While CREST and GOAT are established methods for TS conformer ensemble generation, the associated computational cost remains a major bottleneck in…
Sina Abdollahi, Amir Al Sadi, Marios Kogias, David Kotz + 1 more
—Confidential Virtual Machines (CVMs) are increasingly adopted to protect sensitive workloads from privileged adversaries such as the hypervisor. While they provide strong isolation guarantees, existing CVM architectures lack first-class mechanisms for inter-CVM data sharing due to their disjoint memory model, making…