10 papers · ranked by Valyu relevance
Christina Ludwig, Ludovic Gillet, George Rosenberger, Sabine Amon + 2 more
'Ben C Collins' 'Ruedi Aebersold'] Title: Abstract Many research questions in fields such as personalized medicine, drug screens or systems biology depend on obtaining consistent and quantitatively accurate proteomics data from many samples. SWATH-MS is a specific variant of data-independent acquisition (DIA) methods…
Hannes L. Röst, Ruedi Aebersold, Olga T. Schubert
Targeted mass spectrometry comprises a set of methods able to quantify protein analytes in complex mixtures with high accuracy and sensitivity. These methods, e.g., Selected Reaction Monitoring (SRM) and SWATH MS, use specific mass spectrometric coordinates (assays) for reproducible detection and quantification of…
Chuan-Qi Zhong, Jianfeng Wu, Xingfeng Qiu, Xi Chen + 2 more
'Jiahuai Han'] Targeted SWATH-MS data analysis is critically dependent on the spectral library. Comprehensive spectral libraries of human or several other organisms have been published, but the extensive spectral library for mouse, a widely used model organism is not available. Here, we present a large murine spectral…
Christoph B. Messner, Vadim Demichev, Nic Bloomfield, Matthew White + 7 more
Bridging genotype to phenotype, the proteome has increasingly become of major importance to generate large, longitudinal sample series for data-driven biology and personalized medicine. Major improvements in laboratory automation, chromatography and software have increased the scale and precision of proteomics. So far…
Ben C. Collins, Christie L. Hunter, Yansheng Liu, Birgit Schilling + 21 more
Quantitative proteomics employing mass spectrometry has become an indispensable tool in basic and applied life science research. Methods based on data-dependent acquisition have proved extremely valuable for qualitative proteome analysis but historically have struggled to achieve reproducible quantitative data if large…
Pawel Palmowski, Rachael Watson, G. Nicholas Europe‐Finner, Magdalena Karolczak‐Bayatti + 3 more
'Magdalena Karolczak‐Bayatti' 'Andrew Porter' 'Achim Treumann' 'Michael J. Taggart'] Title: Abstract Advances in liquid chromatography-mass spectrometry have facilitated the incorporation of proteomic studies to many biology experimental workflows. Data-independent acquisition platforms, such as sequential window…
Ziyue Wang, Michael Mülleder, Ihor Batruch, Anjali Chelur + 10 more
The possibility to record proteomes in high throughput and at high quality has opened new avenues for biomedical research, drug discovery, systems biology, and clinical translation. However, high-throughput proteomic experiments often require high sample amounts and can be less sensitive compared to conventional…
Ziyue Wang, Michael Mülleder, Ihor Batruch, Anjali Chelur + 8 more
The ability to conduct high-quality proteomic experiments in high throughput has opened new avenues in clinical research, drug discovery, and systems biology. Next to an increase in quantitative precision, recent developments in high-throughput proteomics have also gained proteomic depth, to the extent that earlier…
Authors not listed
The Single-probe is a multifunctional device that can be coupled to mass spectrometry (MS) for molecular analysis of microscale samples, such as single cells, tissue slices, and multicellular spheroids, under ambient conditions. In Single-probe single cell MS (SCMS) studies, this technique leverages direct sampling and…
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The proper quantitative NMR (qNMR) setup requires efficient relaxation of all active nuclei between scans. To achieve this, the spectroscopist has to know the longitudinal relaxation constant (T1) for each nucleus involved in the experiment and set the interscan delay to at least five times the longest T1. The T1 is…