24 papers · ranked by Valyu relevance
Nicola Gritti, Simone Kienle, Olga Filina, Jeroen Sebastiaan van Zon
We present a microscopy technique that enables long-term time-lapse microscopy at single-cell resolution in moving and feeding Caenorhabditis elegans larvae. Time-lapse microscopy of C. elegans post-embryonic development is challenging, as larvae are highly motile. Moreover, immobilization generally leads to rapid…
Sing Teng Chua, Jurij Kotar, Michael Kühl, Alison G Smith + 2 more
'Silvia Vignolini' 'Pietro Cicuta'] Title: Abstract A planar cell microcolony served as a model system to study the impact of inter-cellular crowding and cell-matrix interactions upon the cell cycle. We studied the development over several days of Chlamydomonas reinhardtii microcolonies, grown from single cells, using…
Guohao Liang, Hong Yin, Fangyuan Ding
Time-lapse microscopy plays critical roles in the studies of cellular dynamics. However, to set up a time-lapse movie experiments is not only laborious but also with low output, mainly due to the cell-losing problem (i.e., cell moving out of limited field of view), especially in a long time recording. To overcome these…
Lionel Chiron, Matthias LeBec, Céline Cordier, Sylvain Pouzet + 5 more
Timelapse fluorescence microscopy imaging is routinely used in quantitative cell biology. However, microscopes could become much more powerful investigation systems if they were endowed with simple unsupervised decision-making algorithms to transform them into fully responsive and automated measurement devices. Here…
Ethan Li, Vittorio Saggiomo, Wei Ouyang, Manu Prakash + 2 more
Microscopy is essential for detecting, identifying, analyzing, and measuring small objects. Access to modern microscopy equipment is crucial for scientific research, especially in the biomedical and analytical sciences. However, the high cost of equipment, limited availability of parts, and challenges associated with…
Eike K. Mahlandt, Joachim Goedhart
One obvious feature of life is that it is highly dynamic. The dynamics can be captured by movies that are made by acquiring images at regular time intervals, a method that is also known as timelapse imaging. Looking at movies is a great way to learn more about the dynamics in cells, tissue and organisms. However…
Lionel Chiron, Matthias Le Bec, Céline Cordier, Sylvain Pouzet + 5 more
'Dimitrije Milunov' 'Alvaro Banderas' 'Jean-Marc Di Meglio' 'Benoit Sorre' 'Pascal Hersen'] Timelapse fluorescence microscopy imaging is routinely used in quantitative cell biology. However, microscopes could become much more powerful investigation systems if they were endowed with simple unsupervised decision-making…
Authors not listed
Scanning ion conductance microscopy (SICM) offers non-contact, label-free imaging of live cells with nanometer-scale resolution. However, its conventional imaging mode is inherently slow due to repeated vertical scanning, limiting temporal resolution and causing inertial issues. Here, we present Scanning Counter Ion…
Clint Gregory, Michael Veeman, Bob Goldstein
Ascidians such as Ciona are close chordate relatives of the vertebrates with small, simple embryonic body plans and small, simple genomes. The tractable size of the embryo offers considerable advantages for in toto imaging and quantitative analysis of morphogenesis. For functional studies, Ciona eggs are considerably…
Quang Long Pham, David Chege, Timothy Dijamco, Migle Surblyte + 4 more
'Akshay Naik' 'Kolawole Campbell' 'Nhat-Anh-Nguyen Tong' 'Roman S. Voronov'] Live time-lapse microscopy is essential for a wide range of biological applications. Software-based automation is the gold standard for the operation of hardware accessories necessary for image acquisition. Given that current software packages…
Samuel M. Leitao, Barney Drake, Katarina Pinjusic, Xavier Pierrat + 9 more
Nanocharacterization plays a vital role in understanding the complex nanoscale organization of cells and organelles. Understanding cellular function requires high-resolution information about how the cellular structures evolve over time. A number of techniques exist to resolve static nanoscale structure of cells in…
Peter Gabriel Pitrone, Johannes Schindelin, Luke Stuyvenberg, Stephan Preibisch + 4 more
'Stephan Preibisch' 'Michael Weber' 'Kevin W. Eliceiri' 'Jan Huisken' 'Pavel Tomančák'] Light sheet microscopy promises to revolutionize developmental biology by enabling live in toto imaging of entire embryos with minimal phototoxicity. We present detailed instructions for building a compact and customizable Selective…
Nimmy S. John, Michelle A. Urman, ChangHwan Lee
Recent advances in microscopy enable three-dimensional live imaging at a high resolution. Long-term live imaging of a multicellular organism requires immobilization of the organism under stable physiological conditions. Despite proper immobilization, challenges remain within live imaging data analysis due to other…
Jonatan Alvelid, Martina Damenti, Ilaria Testa
The observation of protein organization during cellular signalling calls for imaging methods with increased spatial and temporal resolution. STED nanoscopy can access dynamics of nanoscale structures in living cells. However, the available number of recordable frames is often limited by photo-bleaching. Here, we…
Authors not listed
Super-resolution imaging techniques that overcome the diffraction limit of light have gained wide popularity for visual-izing cellular structures with nanometric resolution. Although fluorogenic spirocyclization-structural rhodamine dyes remain the premier type of small-molecule fluorophore for super-resolution imaging…
Q. Tyrell Davis
Microtubules (MTs) are filamentous protein polymers roughly 25 nm in diameter. Ubiquitous in eukaryotes, MTs are well known for their structural role but also act as actuators, sensors, and, in association with other proteins, checkpoint regulators. The thin diameter and transparency of microtubules classifies them as…
Jiangguo Zhang, Jessica A. Comstock, Christopher R. Cotter, Patrick A. Murphy + 4 more
Myxococcus xanthus bacteria are a model system for understanding pattern formation and collective cell behaviors. When starving, cells aggregate into fruiting bodies to form metabolically inert spores. During predation, cells self-organize into traveling cell-density waves termed ripples. Both phase-contrast and…
Kai Long, Wenkai Chen, Junming Zhou, Junyi Li + 5 more
High-speed image acquisition in light microscopy is essential for a wide range of applications, including observing dynamic biological processes and enabling high-throughput sample analysis. However, traditional imaging speeds are often limited by the scanning mechanisms and the signal-to-noise ratio, and these…
Authors not listed
Hydrogen/deuterium exchange (HDX) methods for studying protein dynamics would benefit from millisecond-scale incubations to probe intrinsically disordered proteins, highly dynamic regions and conformation changes. Here we investigate droplet microfluidics for rapid mixing to trigger D2O labelling, uniform incubations…
Karen Grace V. Bondoc, Hannah Laeverenz-Schlogelhofer, Rebecca N. Poon, Alexander K. Boggon + 3 more
'Rebecca N. Poon' 'Alexander K. Boggon' 'Samuel A Bentley' 'Dario Cortese' 'Kirsty Y. Wan'] Motility is an essential factor for an organism's survival and diversification. With the advent of novel single-cell technologies, analytical frameworks and theoretical methods, we can begin to probe the complex lives of…
Melissa E. Swift, Wyatt Ayers, Sophie Pallanck, Scott Wehrwein
The visual smoothness of our pyramid videos stands in stark contrast with the timelapse videos. At the higher levels especially, each frame of the timelapse is far removed in time from its neighboring frames, increasing the likelihood of major discontinuities in lighting, weather, and other large scene elements. The…
Authors not listed
Understanding how plants respond to dynamic and spatially variable stimuli is a key goal in plant sciences. Traditional imaging methods often involve a trade-off between environmental control and spatial resolution, limiting their ability to capture real-time responses in high resolution. Microfluidic technology…
Michael Andrews, Debbie Jones, Adam Woodward, Adam Swinburne + 5 more
Nuclear power is a sustainable zero-carbon energy source but requires generated radionuclide waste be remediated from contaminated lands. Legacy, mining and disposal activities all engender environmental contamination, particularly for uranium, which is the main component by mass being both radioactive and toxic.…
Kanda Borgognoni, Bradley Guilliams, Rachel Cohen, Christopher Ackerson
A cloneable NanoParticle (cNP) is an inorganic nanoparticle that is synthesized by a protein. The protein determines the elemental composition, size, morphology, and other properties of the nanoparticle. Here, we describe the use of a cloneable Selenium NanoParticle (cSeNP) as a cloneable imaging contrast agent in…