24 papers · ranked by Valyu relevance
Zoltán Ivics, Zsuzsanna Izsvák
Transposable elements can be viewed as natural DNA transfer vehicles that, similar to integrating viruses, are capable of efficient genomic insertion. The mobility of class II transposable elements (DNA transposons) can be controlled by conditionally providing the transposase component of the transposition reaction.…
Asmaa H. Hassan, Morad M. Mokhtar, Achraf El Allali
Transposable elements (TEs) are indispensable components of eukaryotic genomes that play diverse roles in gene regulation, recombination, and environmental adaptation. Their ability to mobilize within the genome leads to gene expression and DNA structure changes. TEs serve as valuable markers for genetic and…
Anuj Kumar
Since Barbara McClintock’s groundbreaking discovery of mobile DNA sequences some 70 years ago, transposable elements have come to be recognized as important mutagenic agents impacting genome composition, genome evolution, and human health. Transposable elements are a major constituent of prokaryotic and eukaryotic…
Mei Han, Matthew H. Perkins, Leonardo Santana Novaes, Tao Xu + 1 more
'Hao Chang'] It has been 70 years since Barbara McClintock discovered transposable elements (TE), and the mechanistic studies and functional applications of transposable elements have been at the forefront of life science research. As an essential part of the genome, TEs have been discovered in most species of…
Roshan Prizak, Lennart Hilbert
Transposons, which are DNA sequences that can move to new positions in the genome, make up a large fraction of eukaryotic genomes and occur in clusters. The insertion of transposons into the genome is hindered by compact folding of chromatin, supposedly preventing aberrant or even pathogenic insertion. Chromatin can…
Daniel Heinzelmann, Franziska Reuss, Nikolas Zeh, Robin Nilson + 7 more
Transposases revolutionized the field of genetic engineering, yet the scarcity of functional systems remains a challenge. In response, we conducted a metagenomic screening and identified a novel transposase system from Acyrthosiphon pisum. Through systematic optimization, we enhanced nuclear localization, transposon…
Dylan C. Wallis, Dieu An H. Nguyen, Celja J. Uebel, Carolyn M. Phillips
'Carolyn M. Phillips'] RNA silencing pathways play critical roles in maintaining quiescence of transposons in germ cells to promote genome integrity. However the precise mechanism by which different types of transposons are recognized by these pathways is not fully understood. Furthermore, the location in the germline…
Julie Dazenière, Alexandros Bousios, Adam Eyre-Walker
Transposable elements (TEs) are a major component of most eukaryotic genomes. Here, we present a new approach which allows us to study patterns of natural selection in the evolution of TEs over short time scales. The method uses the alignment of all elements with intact gag/pol genes of a TE family from a single…
Anton G. Henssen, Elizabeth Henaff, Eileen Jiang, Amy R. Eisenberg + 9 more
Transposons are mobile genetic elements that are found in nearly all organisms, including humans. Mobilization of DNA transposons by transposase enzymes can cause genomic rearrangements, but our knowledge of human genes derived from transposases is limited. Here, we find that the protein encoded by human PGBD5, the…
Yukinobu Arata, Peter Jurica, Nicholas Parrish, Yasushi Sako
Transposons are mobile DNA elements that encode genes for their own mobility. Whereas transposon copies accumulate on the genome during evolution, many lose their mobile activity due to mutations. Here, we focus on transposon-encoded genes that are directly involved in the replication, excision, and integration of…
Corentin Claeys Bouuaert, Ronald Chalmers, Beata G. Vertessy
Hsmar1 is a member of the Tc1-mariner superfamily of DNA transposons. These elements mobilize within the genome of their host by a cut-and-paste mechanism. We have exploited the in vitro reaction provided by Hsmar1 to investigate the effect of DNA supercoiling on transposon integration. We found that the topology of…
Yue Wang
With the process of evolution, some genes will change their relative positions in gene sequence. These "jumping genes" are called transposons. Through some intuitive rules, we give a criterion to determine transposons among gene sequences of different individuals of the same species. Then we turn this problem into…
Chi Xue, Nigel Goldenfeld
Transposable elements, or transposons, are DNA sequences that can jump from site to site in the genome during the life cycle of a cell, usually encoding the very enzymes which perform their excision. However, some transposons are parasitic, relying on the enzymes produced by the regular transposons. In this case, we…
Almorò Scarpa, Riccardo Pianezza, Filip Wierzbicki, Robert Kofler
Transposable element invasions have a profound impact on the evolution of genomes and phenotype. It is thus an important open question on how often such TE invasions occur. Based on strains sampled at different times, previous work showed that four different TE families invaded D. melanogaster during the 20^th^…
Ayako Nishizawa-Yokoi, Seiichi Toki
Transposons are mobile genetic elements that can move to a different position within a genome or between genomes. They have long been used as a tool for genetic engineering, including transgenesis, insertional mutagenesis, and marker excision, in a variety of organisms. The piggyBac transposon derived from the cabbage…
Xiu-Ming Guo, Qian-Qian Zhang, Yi-Wen Sun, Xia-Yun Jiang + 1 more
'Shu-Ming Zou'] Tc1-like transposons consist of an inverted repeat sequence flanking a transposase gene that exhibits similarity to the mobile DNA element, Tc1, of the nematode, Caenorhabditis elegans. They are widely distributed within vertebrate genomes including teleost fish; however, few active Tc1-like…
Aria Yom, Nathan E. Lewis
Transposons are tiny intragenomic parasites found in every branch of life. Often, one transposon will hyperparasitize another, forming an intracellular ecosystem. In some species these ecosystems thrive, while in others they go extinct, yet little is known about when or why this occurs. Here, we present a stochastic…
Larry Bull
There is a growing body of work considering the use of representations based upon genetic regulatory networks. This paper uses a recently presented abstract, tunable Boolean regulatory network model to explore aspects of mobile DNA, such as transposons, within these dynamical systems. The significant role of mobile DNA…
Victoria T. Orr, Ellie Harrison, Damian W. Rivett, Rosanna C. T. Wright + 1 more
Plasmids are extrachromosomal mobile genetic elements that can facilitate rapid bacterial adaptation by transferring genes between individuals. While plasmids are known to exist in diverse habitats and encode a range of traits, most of our knowledge about plasmids comes from clinically-associated antimicrobial…
Larry Bull, Andrew Adamatzky
— This paper uses a recently presented abstract, tuneable Boolean regulatory network model extended to consider aspects of mobile DNA, such as transposons. The significant role of mobile DNA in the evolution of natural systems is becoming increasingly clear. This paper shows how dynamically controlling network node…
Alexandros Bousios, Hans-Wilhelm Nuetzmann, Dorothy Buck, Davide Michieletto
'Davide Michieletto'] Chromosome organisation is increasingly recognised as an essential component of genome regulation, cell fate and cell health. Within the realm of transposable elements (TEs) however, the spatial information of how genomes are folded is still only rarely integrated in experimental studies or…
Wei-Kai Hua, Jeff C. Hsu, Yi-Chun Chen, Peter S. Chang + 6 more
Recent advances in gene therapy have brought novel treatment options for cancer. However, the full potential of this approach has yet to be unlocked due to the safety concerns and limited payload capacity of commonly utilized viral vectors. Virus-free DNA transposons, including piggyBac, have potential to obviate these…
Authors not listed
Molecular mechanisms governing initiation steps of the assembly of thousands of endogenous multi-protein complexes (EMCs) remain incompletely understood. Here, multiple lines of observations are reported reflecting the biological functions-aligned initiation sequence of hybrid assembly pathways (HAPs) of EMCs. HAPs…
Authors not listed
In this report, we examine the extensive research landscape of CRISPR with an emphasis on CRISPR therapeutics and showcase our results from an in-depth analysis of the most up-to-date scientific information consisting of more than 53,000 publications encompassing academic journal articles and patents, spanning nearly…