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18 papers · ranked by Valyu relevance
Quanyu Zhou, Daniil Nozdriukhin, Zhenyue Chen, Xosé Luís Deán-Ben + 1 more
The mammalian vascular system is a marvel of biological engineering, comprising an extensive network of vessels that sustains every organ and tissue in the body. Within this network, microvessels smaller than 100 μm-including capillaries, arterioles, and venules-are the principal sites of exchange for oxygen…
Brice Rauby, Nin Ghigo, Gerardo Ramos-Palacios, Alexis Leconte + 12 more
Ultrasound Localization Microscopy (ULM) enables microscopic imaging of the cerebral microvasculature in vivo, but relies on a multi-stage processing pipeline in which acquisition settings and reconstruction processes strongly influence the final output. Existing public datasets remain sparse, restricting rigorous…
Jipeng Yan, Oscar Bates, Jingwen Zhu, Qingyuan Tan + 5 more
Ultrasound Localization Microscopy (ULM) has presented great potential in functional imaging, benefiting from its ability to reconstruct deep microvasculature. However, the hemodynamic reconstruction is compromised by sparsity in the ULM data, as a limited number of MB tracks cannot sample the complete speed profile in…
Cai, Yang, Yan, Shaoyuan + 8 more
Transient brain-wide neuroimaging on a microscopic scale ispivotal for brain research, yet current modalities face challenges in meeting such spatiotemporal requirements. Functional ultrasound (fUS) enables transient neurovascular imaging through red blood cell backscattering, but suffers from diffraction-limited…
Xuyang Chang, Quanyu Zhou, Abigail Vigderman, Shuangyi Cheng + 17 more
Optical, ultrasound, and optoacoustic localization microscopy based on microparticle tracking has enabled surpassing the resolution limits imposed by ultrasound diffraction and optical diffusion in tissues. However, its reliance on high-speed (kilohertz) data acquisition systems for precise emitter localization and…
Ruchika Dhawan, Mihir Agarwal, Shreyans Jain, Himanshu Shekhar
Super-resolution ultrasound (SR-US) reveals microvascular structures with exquisite resolution, but clinical translation remains limited by the need for ultrafast frame rates, massive data volumes, and long reconstruction times. This work proposes a deep learning framework that reconstructs microvascular maps from…
Zhengchang Kou, Junhang Zhang, Chen Gong, Jie Ji + 7 more
Mapping deep microvascular structure and dynamics is essential for understanding organ function, neurovascular coupling and disease progression. Ultrasound localization microscopy (ULM) achieves super-resolution vascular imaging in deep tissue, but its dependence on exogenous contrast agents and prolonged stochastic…
Junlin Chen, Xiaoyu Wang, Jilin Fan, Bi Wang + 16 more
Tracking the fate of transplanted cells in vivo remains a major challenge for the development and monitoring of cell-based therapies. Here, we report a nanobubble (NB) based acoustic labeling strategy that enables ultrasound localization microscopy (ULM) to track transplanted cells in tumors with single-cell-scale…
Zhengchang Kou, Yuning Zhao, Mingrui Liu, Rita J. Miller + 1 more
Ultrasound localization microscopy (ULM) enables micrometer-scale microvascular imaging by localizing and tracking intravascular microbubbles, but its dependence on exogenous contrast agents and long acquisition times limits clinical translation. This study presents a high-frame-rate contrast-free super-resolution…
Hongtong Li, Haiyang Zhan, Rui Ma, Yijun Liu + 6 more
Localization-based super-resolution techniques have revolutionized biomedical imaging by surpassing classical diffraction limits. However, their performance is fundamentally constrained by distortions of the point spread function (PSF) induced by the system and the sample, which are particularly prominent in the case…
Chaorui Qiu, Daiwei Li, Haoming Huo, Arpit Mishra + 10 more
Urinary stone disease is a common urological condition with increasing incidence, particularly in developed countries. Laser lithotripsy (LL) has become a preferred minimally invasive treatment due to its high precision and low tissue damage. Recent studies suggest that cavitation plays a critical role in stone damage…
Tingting Wang, Jiali Chen, Liming Nie, Honghui Li
Photoacoustic microscopy (PAM) has emerged as a versatile modality in biomedical research, notable for its noninvasively, high spatial resolution, and superior optical contrast. Compared with pure optical imaging techniques, PAM leverages weakly scattered ultrasonic signals for image formation, thereby achieving…
Schultze, Steffen, Grubmüller, Helmut
MINFLUX microscopy allows for localization of fluorophores with nanometer precision using targeted scanning with an illumination profile with a minimum. However, current scanning patterns and the overall procedure are based on heuristics, and may therefore be suboptimal. Here we present a rigorous Bayesian that offers…
Dajun Zhang, Hyunji Shim, Michael Ferolito, Feng Ye + 2 more
Dynamic shaping of ultrasound into prescribed spatial patterns underlies a broad range of biomedical and engineering applications. However, existing modulation strategies face fundamental limitations: single element transducers paired with acoustic lenses lack reconfigurability, whereas phased arrays require large…
Johanna Bartmuß, Anke Leinhaas, Tatjana Frank-Wiebe, Marc Schmidt-Supprian + 1 more
We present flow immunofluorescence localization microscopy (FILM) as a novel paradigm for single-molecule localization microscopy (SMLM). FILM is based on continuous flow of standard fluorescent binders, including conventional antibodies or nanoscale probes, over a fixed sample along with gentle…
Yuxin Lin, Haoting Lin, Donggeng Yu, Kevin Welsher + 1 more
The epithelium represents the first line of defense against viral infection, yet the precise mechanisms by which viruses penetrate this complex barrier remain incompletely understood. Single-virus tracking (SVT) has emerged as a powerful fluorescence microscopy approach to directly visualize viral dynamics with…
Siyang Cheng, Nahima Saliba, Gabriella Gagliano, Prakash Joshi + 1 more
Lattice Light Sheet Microscopy with Microfluidics for Single-Molecule Super-Resolution Imaging of Mammalian Cells Authors: Siyang Cheng, Nahima Saliba, Gabriella Gagliano, Prakash Joshi, Anna-Karin Gustavsson Single-molecule localization microscopy (SMLM) has redefined optical imaging by enabling imaging beyond the…
Julius Bernhard Peters, Christopher Heidebrecht, Michael Weber, Marcel Leutenegger + 1 more
Investigating the movements and conformational changes of proteins in living cells is essential for understanding their function. The recently introduced fluorescence nanoscopy method called MINSTED has successfully tracked single fluorophore-labelled proteins, albeit in fixed cells and two dimensions only. Here we…