21 papers · ranked by Valyu relevance
Meifang Qi, Utthara Nayar, Leif S. Ludwig, Nikhil Wagle + 1 more
Exogenous cDNA introduced into an experimental system, either intentionally or accidentally, can appear as added read coverage over that gene in next-generation sequencing libraries derived from this system. If not properly recognized and managed, this cross-contamination with exogenous signal can lead to incorrect…
Peng Ling, Meinan Wang, Xianming Chen, Kimberly Garland Campbell
Background Puccinia striiformis is a plant pathogenic fungus causing stripe rust, one of the most important diseases on cereal crops and grasses worldwide. However, little is know about its genome and genes involved in the biology and pathogenicity of the pathogen. We initiated the functional genomic research of the…
Beatriz Lorenzo, Francisco J. González‐Castaño, Yuguang Fang
—Increasing mobile data demands in current cellular networks and proliferation of advanced handheld devices have given rise to a new generation of dynamic network architectures (DNAs). In a DNA, users share their connectivities and act as access points providing Internet connections for others without additional…
Meifang Qi, Utthara Nayar, Leif S. Ludwig, Nikhil Wagle + 1 more
'Esther Rheinbay'] Background Exogenous cDNA introduced into an experimental system, either intentionally or accidentally, can appear as added read coverage over that gene in next-generation sequencing libraries derived from this system. If not properly recognized and managed, this cross-contamination with exogenous…
Arne Weiberg, Dirk Pöhler, Burkhard Morgenstern, Petr Karlovsky
Background cDNA-AFLP is a transcriptomics technique which does not require prior sequence information and can therefore be used as a gene discovery tool. The method is based on selective amplification of cDNA fragments generated by restriction endonucleases, electrophoretic separation of the products and comparison of…
Reginaldo M. Kuroshu, Junichi Watanabe,, Sumio Sugano, Shinichi Morishita + 3 more
'Shinichi Morishita' 'Yutaka Suzuki' 'Masahiro Kasahara' 'Alfredo Herrera-Estrella'] Background Sequencing full-length cDNA clones is important to determine gene structures including alternative splice forms, and provides valuable resources for experimental analyses to reveal the biological functions of coded proteins.…
Felix Grünberger, Sébastien Ferreira-Cerca, Dina Grohmann
High-throughput sequencing dramatically changed our view of transcriptome architectures and allowed for ground-breaking discoveries in RNA biology. Recently, sequencing of full-length transcripts based on the single-molecule sequencing platform from Oxford Nanopore Technologies (ONT) was introduced and is widely…
Mandana Mirbakhsh
The oldest evidence about utilization of Saffron dates back to 2,000-1,500 years BC. Saffron is a triploid sterile species that is vegetatively propagation with Corm that only survives for only one season,reproducing via division into "cormlets" that eventually give rise to new plant and took time at least one year for…
R. M. Farouk, Sherif M. Badr, M. Sayed Elahl
The complementary DNA (cDNA) sequence is considered to be the magic biometric technique for personal identification. In this paper, we present a new method for cDNA recognition based on the artificial neural network (ANN). Microarray imaging is used for the concurrent identification of thousands of genes. We have…
G. Nallappa Bhavithran, R. Selvakumar
The biggest challenge when using DNA as a storage medium is maintaining its stability. The relative occurrence of Guanine (G) and Cytosine (C) is essential for the longevity of DNA. In addition to that, reverse complementary base pairs should not be present in the code. These challenges are overcome by a proper choice…
Balakrishnan Venkatesh, Ursula Hettwer, Birger Koopmann, Petr Karlovský
'Petr Karlovský'] Background: Gene expression studies on non-model organisms require open-end strategies for transcription profiling. Gel-based analysis of cDNA fragments allows to detect alterations in gene expression for genes which have neither been sequenced yet nor are available in cDNA libraries. Commonly used…
Yoshie Shimauchi-Matsukawa, Yoshinobu Aman, Shuji Tachibanaki, Satoru Kawamura
'Satoru Kawamura'] Purpose Rods and cones differ in their photoresponse characteristics, morphology, and susceptibilities to certain diseases. To contribute to the studies at the molecular level of these differences, we tried to identify genes expressed preferentially in rods or cones. Methods From purified carp rods…
Gen Hua Yue, Ze Yuan Zhu, Chun Ming Wang, Jun Hong Xia
Background Microsatellites in cDNA are useful as molecular markers because they represent transcribed genes and can be used as anchor markers for linkage and comparative mapping, as well as for studying genome evolution. Microsatellites in cDNA can be detected in existing ESTs by data mining. However, in most fish…
Dixita Limbachiya, Bansari Rao, Manish K. Gupta
The idea of computing with DNA was given by Tom Head in 1987, however in 1994 in a seminal paper, the actual successful experiment for DNA computing was performed by Adleman. The heart of the DNA computing is the DNA hybridization, however, it is also the source of errors. Thus the success of the DNA computing depends…
Man Zhang, Lei Ye
Using rolling circle amplification (RCA) and two different ways of signal readout, we developed analytical methods to detect the receptor binding domain (RBD) of SARS-CoV 2 spike protein (S protein). We modified streptavidin-coated magnetic beads with an aptamer of RBD through a biotin-tagged complementary DNA strand…
HANIYEH ABDOLLAHZADEH, Tonya Peeples, Mohammad Shahcheraghi
DNA-based nanomaterials have shown great potential in numerous applications, thanks to their unique properties including DNA's various molecular interactions, programmability, and versatility with biological modules. Meanwhile, the DNA origami platforms have shown promise in the creation of drug carriers. This…
Shabirul Haque, Sarah R. Vaiselbuh
Exosomes are nanovesicles (∼30-150 nm diameters) released via an endocytic pathway in almost all mammalian cell types. Exosomes are composed of a lipid bilayer membrane that encloses RNA, miRNA, proteins and DNA. This manuscript unravels how exosome cargo is collected by a highly precise process delineating two…
Sarangdhar Nayak, Pawan K. Dhar
The dark genome comprising of non-expressing, non-translating, and extinct DNA sequences has remained a largely unexplored genomic space. Using computational and experimental approaches, novel insights into the dark matter genome have recently been gained, revealing the presence of a vast and unexplored resource.…
Authors not listed
Large-scale de novo nucleic acid synthesis is a powerful tool enabling researchers to better understand and engineer biological systems. Fields ranging from genomics to nucleic acid therapeutics to synthetic biology make use of high-throughput experimental approaches requiring access to large pools or libraries of DNA…
Jürgen Behr, Timm Michel, Maya Giridhar, Santra Santhosh + 11 more
Large- to ultra-large-scale synthesis of nucleic acids is becoming an increasingly important tool for understanding and manipulating biological systems, as well as for developing new technologies based on engineered biological materials, including DNA-based nanofabrication, aptamers and writing digital data at the…
Aaron M. Love, Nikhil U. Nair
As cellular engineering progresses from simply overexpressing proteins to imparting complex metabolic and regulatory phenotypes through multigene expression, judicious appropriation of cellular resources is essential. Since there is degeneracy in codons and their use is biased, codons may control cellular resources at…