20 papers · ranked by Valyu relevance
Yu-Lin Chen, Syon Reddy, Aussie Suzuki
The cell cycle is a crucial process for cell proliferation, differentiation, and development. Numerous genes and proteins play pivotal roles at specific cell cycle stages to regulate these events precisely. Studying the stage-specific functions of the cell cycle requires accumulating cell populations at the desired…
Jie Yan, Albert Goldbeter
The cell cycle and the circadian clock represent major cellular rhythms, which appear to be coupled. Thus the circadian factor BMAL1 controls the level of cell cycle proteins such as Cyclin E and WEE1, the latter of which inhibits the kinase CDK1 that governs the G2/M transition. In reverse the cell cycle impinges on…
Eleanor Wendy Trotter, Iain Michael Hagan
Each approach used to synchronize cell cycle progression of human cell lines presents a unique set of challenges. Induction synchrony with agents that transiently block progression through key cell cycle stages are popular, but change stoichiometries of cell cycle regulators, invoke compensatory changes in growth rate…
Wei Zhang, Xiufen Zou
Background The biochemical oscillator that controls periodic events during the Xenopus embryonic cell cycle is centered on the activity of CDKs, and the cell cycle is driven by a protein circuit that is centered on the cyclin-dependent protein kinase CDK1 and the anaphase-promoting complex (APC). Many studies have been…
Manuela Vecsler, Itay Lazar, Amit Tzur, Michael Polymenis
Cell cycle research greatly relies on synchronization of proliferating cells. However, effective synchronization of mammalian cells is commonly achieved by long exposure to one or more cell cycle blocking agents. These chemicals are, by definition, hazardous (some more than others), pose uneven cell cycle arrest, thus…
Stephen Cooper
Background The paper of Liu, Gaido and Wolfinger on gene expression during the division cycle of HeLa cells using the data of Whitfield et al. are discussed in order to see whether their analysis is related to gene expression during the division cycle. Results The results of Liu, Gaido and Wolfinger demonstrate that…
Chance M. Nowak, Tyler Quarton, Leonidas Bleris
The cell cycle consists of a series of orchestrated events controlled by molecular sensing and feedback networks that ultimately drive the duplication of total DNA and the subsequent division of a single parent cell into two daughter cells. The ability to block the cell cycle and synchronize cells within the same phase…
Peter Boldog, Gergely Röst
We demonstrate the feasibility of our model by fitting it to experimental data from melanoma cell lines previously studied by Vittadello et al. Notably, our model successfully replicates experimentally observed synchronization phenomena that multi-stage models could not adequately explain. By using a gamma distribution…
Nica Gutu, Malthe S. Nordentoft, Marlena Kuhn, Carolin Ector + 7 more
Single-cell circadian oscillators exchange extracellular information to sustain coherent circadian rhythms at the tissue level. Within cells, the circadian clock and the cell cycle couple, yet the mechanisms governing this interplay remain poorly elucidated. Here, we study the role of extracellular circadian…
Yihan Lin, Ying Li, Sean Crosson, Aaron R. Dinner + 1 more
'Norbert F. Scherer'] Genomic and proteomic methods yield networks of biological regulatory interactions but do not provide direct insight into how those interactions are organized into functional modules, or how information flows from one module to another. In this work we introduce an approach that provides this…
Daria Shlyueva, Sarah Teed-Singer, Yingqian Zhan, Richard Koche + 1 more
The cellular and nuclear states are constantly changing and closely linked to DNA replication and cell division, defining cell cycle phases. Pharmacological inhibition is a widely used strategy to enrich for specific phases to study them in isolation. However, chemical-induced cell cycle synchronization may influence…
A. Chervov, Andreï Zinovyev
The cell cycle is one of the most fundamental biological processes important for understanding normal physiology and various pathologies such as cancer. Single cell RNA sequencing technologies give an opportunity to analyse the cell cycle transcriptome dynamics in an unprecedented range of conditions (cell types and…
Vince Kornél Grolmusz, Eszter Angéla Tóth, Kornélia Baghy, István Likó + 5 more
'István Likó' 'Ottó Darvasi' 'Ilona Kovalszky' 'János Matkó' 'Károly Rácz' 'Attila Patócs'] Background Previously, drug-based synchronization procedures were used for characterizing the cell cycle dependent transcriptional program. However, these synchronization methods result in growth imbalance and alteration of the…
Nikolai Slavov, David Botstein, Amy A. Caudy
Yeast cells grown in culture can spontaneously synchronize their respiration, metabolism, gene expression and cell division. Such metabolic oscillations in synchronized cultures reflect single-cell oscillations, but the relationship between the oscillations in single cells and synchronized cultures is poorly…
Nikolai Slavov, David Botstein, Amy Caudy
Yeast cells grown in culture can spontaneously synchronize their respiration, metabolism, gene expression and cell division. Such metabolic oscillations in synchronized cultures reflect single-cell oscillations, but the relationship between the oscillations in single cells and synchronized cultures is poorly…
Martin Mojica-Benavides, David D. van Niekerk, Mite Mijalkov, Jacky L. Snoep + 4 more
'Jacky L. Snoep' 'B. Mehlig' 'Giovanni Volpe' 'Mattias Goksör' 'Caroline B. Adiels'] Metabolic oscillations in single cells underlie the mechanisms behind cell synchronization and cell-cell communication. For example, glycolytic oscillations mediated by biochemical communication between cells may synchronize the…
Authors not listed
The WRN helicase has recently emerged as a promising therapeutic target for microsatellite instability (MSI)-high cancers. Here, we report LXW-P1, a potent WRN degrader derived from marine bromotyrosine alkaloids. Its molecular target was identified using an AI-guided, pathway-informed perturbation transcriptomics…
Authors not listed
Long-lived and photostable fluorescent probes for real-time monitoring of cell division, particularly for dynamic visualization of mitosis and interphase, are rare. We have developed a water soluble diazaoxatriangulenium cation based fluorescent probe, Nuc-DAOTA+, that meets these criteria. Nuc-DAOTA+ has a long…
Kanda Borgognoni, Bradley Guilliams, Rachel Cohen, Christopher Ackerson
A cloneable NanoParticle (cNP) is an inorganic nanoparticle that is synthesized by a protein. The protein determines the elemental composition, size, morphology, and other properties of the nanoparticle. Here, we describe the use of a cloneable Selenium NanoParticle (cSeNP) as a cloneable imaging contrast agent in…
Juerg Straubhaar, Alexandria D’Souza, Zachary Niziolek, Bogdan Budnik
Single-cell analysis has clearly established itself in biology and biomedical fields as an invaluable tool that allows one to comprehensively understand the relationship between cells, including their types, states, transitions, trajectories, and spatial position. Scientific methods such as fluorescence labeling…