22 papers · ranked by Valyu relevance
Manuel Seefelder, Stefan Kochanek, Fabrice A. C. Klein
Colocalization, the spatial overlap of molecular entities, is often key to support their involvement in common functions. Existing colocalization tools, however, face limitations, particularly because of their basic statistical analysis and their low-throughput manual entry processes making them unsuitable for…
Julia Naas, Giacomo Nies, Housen Li, Stefan Stoldt + 3 more
'Bernhard Schmitzer' 'Stefan Jakobs' 'Axel Munk'] With recent advances in multi-color super-resolution light microscopy, it is possible to simultaneously visualize multiple subunits within biological structures at nanometer resolution. To optimally evaluate and interpret spatial proximity of stainings on such an image…
Ilaria De Santis, Luca Lorenzini, Marzia Moretti, Elisa Martella + 6 more
'Enrico Lucarelli' 'Laura Calzà' 'Alessandro Bevilacqua' 'Sheryl Berlin Brahnam' 'Loris Nanni' 'Rick Brattin'] Cellular and subcellular spatial colocalization of structures and molecules in biological specimens is an important indicator of their co-compartmentalization and interaction. Presently, colocalization in…
Yasuhiro Kojima, Shinji Mii, Shuto Hayashi, Haruka Hirose + 4 more
Analyzing colocalization of single cells with heterogeneous molecular phenotypes is essential for understanding cell-cell interactions, cellular responses to external stimuli, and their biological functions in diseases and tissues. However, high-throughput methods for identifying spatial proximity at single-cell…
Aske L. Ejdrup, Matthew D. Lycas, Niels Lorenzen, Ainoa Konomi + 3 more
'Freja Herborg' 'Kenneth L. Madsen' 'Ulrik Gether'] Dual-color single-molecule localization microscopy (SMLM) provides unprecedented possibilities for detailed studies of colocalization of different molecular species in a cell. However, the informational richness of the data is not fully exploited by current analysis…
Aske L. Ejdrup, Matthew D. Lycas, Niels Lorenzen, Ainoa Konomi + 3 more
Dual-color single-molecule localization microscopy (SMLM) provides unprecedented possibilities for detailed studies of colocalization of different molecular species in a cell. However, the informational richness of the data is not fully exploited by current analysis tools that often reduce colocalization to a single…
Shulei Wang, Ellen T. Arena, Kevin W. Eliceiri, Ming Yuan
—Colocalization is a powerful tool to study the interactions between fluorescently labeled molecules in biological fluorescence microscopy. However, existing techniques for colocalization analysis have not undergone continued development especially in regards to robust statistical support. In this paper, we examine two…
Jorge Ignacio Villalta, Soledad Galli, María Florencia Iacaruso, Valeria Gabriela Antico Arciuch + 4 more
The subcellular localization and physiological functions of biomolecules are closely related and thus it is crucial to precisely determine the distribution of different molecules inside the intracellular structures. This is frequently accomplished by fluorescence microscopy with well-characterized markers and posterior…
Luciano da Fontoura Costa
Given two or more concentrations, an interesting and important related issue concerns the quantification of how strongly they are spatially interrelated. The concept of colocalization has been frequently considered as an indication of the tendency of the values of two concentrations to spatially vary together. While…
Shulei Wang, Ellen T. Arena, Jordan T. Becker, William M. Bement + 3 more
'Nathan M. Sherer' 'Kevin W. Eliceiri' 'Ming Yuan'] Abstract—Colocalization analysis aims to study complex spatial associations between bio-molecules via optical imaging techniques. However, existing colocalization analysis workflows only assess an average degree of colocalization within a certain region of interest…
Jesus Vega-Lugo, Bruno da Rocha-Azevedo, Aparajita Dasgupta, Nicolas Touret + 1 more
Colocalization analysis of multicolor microscopy images is a cornerstone approach in cell biology. It provides information on the localization of molecules within various subcellular compartments and allows the interrogation of molecular interactions in their cellular context. However, almost all colocalization…
Shuaibin Lian, Tianliang Liu, Shengli Jing, Hongyu Yuan + 2 more
'Zaibao Zhang' 'Lin Cheng'] Background Gene order and location in chromosomes of species are non-random. Neighboring gene pairs tend to display some similarities, such as co-expression and co-modification. However, the contribution of linear proximity, spatial proximity, and evolutionary proximity to these similarities…
Abhay Hukku, Milton Pividori, Francesca Luca, Roger Pique-Regi + 2 more
Colocalization analysis has emerged as a powerful tool to uncover the overlapping of causal variants responsible for both molecular and complex disease phenotypes. The findings from colocalization analysis yield insights into the molecular pathways of complex diseases. In this paper, we conduct an in-depth…
Zulzikry Hafiz Abu Bakar, Jean-Pierre Bellier, Wan Zurinah Wan Ngah, Daijiro Yanagisawa + 3 more
'Daijiro Yanagisawa' 'Ken-ichi Mukaisho' 'Ikuo Tooyama' 'J. Bernard Heymann'] The precision of colocalization analysis is enhanced by 3D and is potentially more accurate than 2D. Even though 3D improves the visualization of colocalization analysis, rendering a colocalization model may generate a model with numerous…
Ashish Patel, John C. Whittaker, Stephen Burgess
relevant genetic variants Authors: ['Ashish Patel' 'John C. Whittaker' 'Stephen Burgess'] Colocalization analyses assess whether two traits are affected by the same or distinct causal genetic variants in a single gene region. A class of Bayesian colocalization tests (Giambartolomei et al., 2014; Wallace, 2021) are now…
Hang Hu, Jyothsna Padmakumar Bindu, Julia Laskin
Mass spectrometry imaging (MSI) is widely used for the label-free molecular mapping of biological samples. The identification of co-localized molecules in MSI data is crucial to the understanding of biochemical pathways. However, complex MSI data are too large for manual annotation but too small for training deep…
Authors not listed
Quantitative analysis of small extracellular vesicles (sEVs) at single-particle resolution remains challenging due to their nanoscale dimensions and compositional heterogeneity. Existing methods often rely on specialized and costly instrumentation, limiting accessibility for many researchers and necessitates extensive…
Solmaz S. Kia, Stephen F. Rounds, Sonia Martı́nez
Technological advances in ad-hoc networking and miniaturization of electro-mechanical systems are making possible the use of large numbers of mobile agents (e.g., mobile robots, human agents, unmanned vehicles) to perform surveillance, search and rescue, transport and delivery tasks in aerial, underwater, space, and…
Yujie Hu, Yu Zhang, Kyle Shelton
Pedestrians and cyclists are vulnerable road users. They are at greater risk for being killed in a crash than other road users. The percentage of fatal crashes that involve a pedestrian or cyclist is higher than the overall percentage of total trips taken by both modes. Because of this risk, finding ways to minimize…
Xia Wang, Zeyu Liu, Xiufen Yan, Tian Lu + 1 more
Although the unique cyclo[18]carbon (C18) realized by recent experiments has been greatly concerned, it has so far remained elusive. In contrast, its precursors C18-(CO)n (n = 6, 4, and 2), which can be separated stably, are of more practical significance. In this paper, the bonding character, electron delocalization…
Xia Wang, Zeyu Liu, Jiaojiao Wang, Tian Lu + 3 more
The stable precursor C18Br6 is of more practical significance than the refreshing but elusive cyclo[18]carbon (C18) at present, and molecular aromaticity caused by π-electron delocalization is an important issue of interest for this unusual molecule. In this paper, the electronic structure and bonding character of the…
Authors not listed
The increasing interest in protein and peptide coacervates is accompanied by the development of various applications, from drug delivery to biosensor preparation. However, the impact of peptide end groups and charges on the coacervation remains unclear. For this purpose, we designed four peptide derivatives with…