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Search · four archives
25 papers · ranked by Valyu relevance
Pascale Schellenberger, Rainer Kaufmann, C. Alistair Siebert, Christoph Hagen + 2 more
'Christoph Hagen' 'Harald Wodrich' 'Kay Grünewald'] Correlative light and electron microscopy (CLEM) is an emerging technique which combines functional information provided by fluorescence microscopy (FM) with the high-resolution structural information of electron microscopy (EM). So far, correlative cryo microscopy of…
Sotatsu Yanagimoto, Naoki Yamamoto, Tatsuro Yuge, Hikaru Saito + 2 more
'K. Akiba' 'Takumi Sannomiya'] Electron microscopy based on high-energy electrons allows nanoscopic analytical imaging taking advantage of secondarily generated particles. Especially for cathodoluminescence, the correlation between primary incident electrons and emitted photons includes information on the entire…
Jie E. Yang, Matthew R. Larson, Bryan S. Sibert, Samantha Shrum + 1 more
Cryo-correlative light and electron microscopy (CLEM) is a technique that uses the spatiotemporal cues from fluorescence light microscopy (FLM) to investigate the high-resolution ultrastructure of biological samples by cryo-electron microscopy (cryo-EM). Cryo-CLEM provides advantages for identifying and distinguishing…
Heather L. Struckman, Nicolae Moise, Bieke Vanslembrouck, Nathan Rothacker + 4 more
Correlative light and electron microscopy (CLEM) methods are powerful methods which combine molecular organization (from light microscopy) with ultrastructure (from electron microscopy). However, CLEM methods pose high cost/difficulty barriers to entry and have very low experimental throughput. Therefore, we have…
Doory Kim, Thomas J. Deerinck, Yaron M. Sigal, Hazen P. Babcock + 3 more
'Mark H. Ellisman' 'Xiaowei Zhuang' 'Michael A. Fox'] Correlative fluorescence light microscopy and electron microscopy allows the imaging of spatial distributions of specific biomolecules in the context of cellular ultrastructure. Recent development of super-resolution fluorescence microscopy allows the location of…
Michael Radermacher, Teresa Ruiz
The influence of noise on cross-correlations is revisited. Equations are provided describing the influence of noise on the cross-correlations between single images and averaged images and on those between averaged images.
José María Mateos, Gery Barmettler, Jana Doehner, Andres Kaech + 1 more
'Urs Ziegler'] A simple method for imaging biological tissue samples by electron microscopy and its correlation with super-resolution light microscopy is presented. This room temperature protocol, based on protecting thin biological specimens with methylcellulose and imaging with low voltage scanning electron…
Kanda Borgognoni, Bradley Guilliams, Rachel Cohen, Christopher Ackerson
A cloneable NanoParticle (cNP) is an inorganic nanoparticle that is synthesized by a protein. The protein determines the elemental composition, size, morphology, and other properties of the nanoparticle. Here, we describe the use of a cloneable Selenium NanoParticle (cSeNP) as a cloneable imaging contrast agent in…
Daen Jannis, Knut Müller‐Caspary, Armand Béché, A. Oelsner + 1 more
'Johan Verbeeck'] We demonstrate the feasibility of coincidence measurements in a conventional transmission electron microscope, revealing the temporal correlation between electron energy loss spectroscopy (EELS) and energy dispersive X-ray (EDX) spectroscopy events. We make use of a delay line detector with picosecond…
Ji-Hwan Kwon, Joohyun Lee, Je In Lee, Byeong-Gwan Cho + 2 more
'Sooheyong Lee' 'Linda J. Johnston'] We investigate the coherence properties of a transmission electron microscope by analyzing nano-diffraction speckles originating from bulk metallic glass. The spatial correlation function of the coherent diffraction patterns, obtained in the transmission geometry, reveals the highly…
Boris Louis, Sudipta Seth, Qingzhi An, Ran Ji + 3 more
Understanding the relationship between the active material's structure and function in operational devices is key for the rational engineering of the next generation of devices. However, this has proven elusive due to the invasiveness of structural characterization methods and their inability to penetrate the…
Suyeon Yang, Machi Takeuchi, Rick Joosten, John van Duynhoven + 2 more
Lipid oxidation is a major cause of product deterioration in protein stabilised oil-in-water food emulsions. The impact of protein emulsifiers on lipid oxidation and the stability depends on the specific type of protein emulsifiers used and the redox conditions in the emulsion. However, the exact impact of these…
Joseph R. Hamiliton, Summer K. Levis, Guy M. Hagen
Correlative microscopy techniques are used for many different applications in the biological sciences because the comparison of different imaging methods allows researchers to gain more insight and data from samples. Correlative light and electron microscopy (CLEM) methods have been developed to preserve biological…
Vivian Tong, T. Ben Britton
Electron backscatter diffraction (EBSD) in the scanning electron microscope is routinely used for microstructural characterisation of polycrystalline materials. Maps of EBSD data are typically acquired at high stage tilt and slow scan speed, leading to tilt and drift distortions that obscure or distort features in the…
M. Haroon Qaiser, Lukas Berners, Robin J. Scales, Tianbi Zhang + 5 more
Electron channeling contrast imaging (ECCI) is a scanning electron microscopy (SEM) based technique that enables bulk-sample characterization of crystallographic defects (e.g. dislocations, stacking faults, low angle boundaries). Despite its potential, ECCI remains underused for quantitative defect analysis as compared…
R. Bromberg, Y. Guo, D. Borek, Z. Otwinowski
The correct description of quantum scattering places the observed scattering contributions on the Ewald’s sphere and its Friedel mate copy. In electron microscopy, due to the large radius of the Ewald’s sphere, these scattering contributions are typically merged during data analysis. We present an approach that…
Leonid A. Bendersky, Frank W. Gayle
Electron diffraction via the transmission electron microscope is a powerful method for characterizing the structure of materials, including perfect crystals and defect structures. The advantages of electron diffraction over other methods, e.g., x-ray or neutron, arise from the extremely short wavelength (≈2 pm), the…
Peter S. Neu, Daniël Geelen, Aniket Thete, R. M. Tromp + 1 more
'Sense Jan van der Molen'] Transmission electron microscopy at very low energy is a promising way to avoid damaging delicate biological samples with the incident electrons, a known problem in conventional transmission electron microscopy. For imaging in the 0-30 eV range, we added a second electron source to a low…
Ka Man Yip, Niels Fischer, Elham Paknia, Ashwin Chari + 1 more
Single particle cryo-EM is a powerful method to solve the three-dimensional structures of biological macromolecules. The technological development of electron microscopes, detectors, automated procedures in combination with user friendly image processing software and ever-increasing computational power have made…
Jinlong Zhu, Lenan Zhang, Xiangyu Li, Kyle L. Wilke + 2 more
'Lynford L. Goddard'] Title: Abstract Environmental scanning electron microscopy (ESEM) is a powerful technique that enables imaging of diverse specimens (e.g., biomaterials, chemical materials, nanomaterials) in a hydrated or native state while simultaneously maintaining micro-to-nanoscale resolution. However, it is…
Xujing Li, Ivan Lazić, Xiaojun Huang, Li Wang + 6 more
Scanning transmission electron microscopy (STEM) is a powerful imaging technique and has been widely used in current material science research. The attempts of applying STEM into biological research have been going on for decades while applications have still been limited because of the existing bottlenecks in dose…
O. S. Sokolova, T. S. Trifonova, N. I. Derkacheva, A. V. Moiseenko
Analytical electron microscopy techniques, including energy-dispersive X-ray spectroscopy (EDX) and electron energy-loss spectroscopy (EELS), are employed in materials science and biology to visualize and chemically map diverse elements. This review presents cases of successful identification of nucleic acids in cells…
Authors not listed
Electron diffraction (MicroED/3DED) facilitates atomic structure determination from nano- or microcrystals, allowing structural analysis of dry crystalline powders or mixtures. However, radiation damage and instrument vacuum limit the interrogation of molecular crystals in solution at room temperature. We now present a…
Aniruddha Acharya
This comprehensive review discusses the development of scanning electron microscopy and the application of this technology in different fields such as biology, nanobiotechnology and biomedical science. Besides being a tool for high resolution imaging of surface or topography, the technology is coupled with analytical…
Niko Vlahakis, Arden Clauss, Jose Rodriguez
High-energy electrons induce sample damage and motion at the nanoscale to fundamentally limit determination of molecular structures by electron diffraction. Using fast event-based electron counting (EBEC) detectors, we characterize electron beam-induced crystal lattice reorientations (BIR) that appear to broadly affect…