Search · four archives
Search · four archives
24 papers · ranked by Valyu relevance
Sotatsu Yanagimoto, Naoki Yamamoto, Tatsuro Yuge, Hikaru Saito + 2 more
'K. Akiba' 'Takumi Sannomiya'] Electron microscopy based on high-energy electrons allows nanoscopic analytical imaging taking advantage of secondarily generated particles. Especially for cathodoluminescence, the correlation between primary incident electrons and emitted photons includes information on the entire…
Heather L. Struckman, Nicolae Moise, Bieke Vanslembrouck, Nathan Rothacker + 4 more
Correlative light and electron microscopy (CLEM) methods are powerful methods which combine molecular organization (from light microscopy) with ultrastructure (from electron microscopy). However, CLEM methods pose high cost/difficulty barriers to entry and have very low experimental throughput. Therefore, we have…
Marit de Beer, Deniz Daviran, Rona Roverts, Luco Rutten + 4 more
'Elena Macías-Sánchez' 'Juriaan R. Metz' 'Nico Sommerdijk' 'Anat Akiva'] Cryo-correlative light and electron microscopy (cryoCLEM) is a powerful strategy to high resolution imaging in the unperturbed hydrated state. In this approach fluorescence microscopy aids localizing the area of interest, and cryogenic focused ion…
Rudolf Haindl, Valerio Di Giulio, Armin Feist, Claus Ropers
pulses Authors: ['Rudolf Haindl' 'Valerio Di Giulio' 'Armin Feist' 'Claus Ropers'] Coulomb interactions impose fundamental limits on the brightness of continuous and pulsed electron beams. When multiple electrons leave a field emitter within a space-time volume of femtoseconds and nanometers, their mutual repulsion…
Kanda Borgognoni, Bradley Guilliams, Rachel Cohen, Christopher Ackerson
A cloneable NanoParticle (cNP) is an inorganic nanoparticle that is synthesized by a protein. The protein determines the elemental composition, size, morphology, and other properties of the nanoparticle. Here, we describe the use of a cloneable Selenium NanoParticle (cSeNP) as a cloneable imaging contrast agent in…
Johannes Groen, Anastasia Gazi, Sergey Kapishnikov, Anne Brelot + 4 more
Cryo-imaging in cellular biology provides the means to visualize the cellular interieur at close-to-native conditions. A cornerstone in the field has been cryo-correlative light and electron microscopy (cryo-CLEM), with cryo-visible light fluorescent microscopy (cryo-VLFM) providing the specificity by tagging…
Ji-Hwan Kwon, Joohyun Lee, Je In Lee, Byeong-Gwan Cho + 2 more
'Sooheyong Lee' 'Linda J. Johnston'] We investigate the coherence properties of a transmission electron microscope by analyzing nano-diffraction speckles originating from bulk metallic glass. The spatial correlation function of the coherent diffraction patterns, obtained in the transmission geometry, reveals the highly…
Anna M. Steyer, Dietrich W. M. Walsh, Euan Pyle, Nadav Scher + 2 more
Cryo-correlative light and electron microscopy methods enable targeted structural analysis of fluorescently labelled features in vitrified specimens. However, correlative workflows on high-pressure frozen samples often remain challenging due to the lack of persistent landmarks for reliable sample tracking and image…
Boris Louis, Sudipta Seth, Qingzhi An, Ran Ji + 3 more
Understanding the relationship between the active material's structure and function in operational devices is key for the rational engineering of the next generation of devices. However, this has proven elusive due to the invasiveness of structural characterization methods and their inability to penetrate the…
Raku Son, Kenji Yamazawa, Akiko Oguchi, Mitsuo Suga + 5 more
'Motoko Yanagita' 'Yasuhiro Murakawa' 'Satoshi Kume' 'Jiarui Wu'] Title: Abstract The living body is composed of innumerable fine and complex structures. Although these structures have been studied in the past, a vast amount of information pertaining to them still remains unknown. When attempting to observe these…
Suyeon Yang, Machi Takeuchi, Rick Joosten, John van Duynhoven + 2 more
Lipid oxidation is a major cause of product deterioration in protein stabilised oil-in-water food emulsions. The impact of protein emulsifiers on lipid oxidation and the stability depends on the specific type of protein emulsifiers used and the redox conditions in the emulsion. However, the exact impact of these…
Ye-Jin Kim, Hak-Won Nho, Shaozheng Ji, Hyejin Lee + 3 more
'Jonas Weissenrieder' 'Oh-Hoon Kwon'] Using an energy filter in transmission electron microscopy has enabled elemental mapping at the atomic scale and improved the precision of structural determination by gating inelastic and elastic imaging electrons, respectively. Here, we use an energy filter in ultrafast electron…
Joseph R. Hamiliton, Summer K. Levis, Guy M. Hagen
Correlative microscopy techniques are used for many different applications in the biological sciences because the comparison of different imaging methods allows researchers to gain more insight and data from samples. Correlative light and electron microscopy (CLEM) methods have been developed to preserve biological…
M. Haroon Qaiser, Lukas Berners, Robin J. Scales, Tianbi Zhang + 5 more
Electron channeling contrast imaging (ECCI) is a scanning electron microscopy (SEM) based technique that enables bulk-sample characterization of crystallographic defects (e.g. dislocations, stacking faults, low angle boundaries). Despite its potential, ECCI remains underused for quantitative defect analysis as compared…
R. Bromberg, Y. Guo, D. Borek, Z. Otwinowski
The correct description of quantum scattering places the observed scattering contributions on the Ewald’s sphere and its Friedel mate copy. In electron microscopy, due to the large radius of the Ewald’s sphere, these scattering contributions are typically merged during data analysis. We present an approach that…
Sasha E. Alden, Oluwasegun J. Wahab, Lingjie Zhang, Kelly L. Vernon + 4 more
Carbon–Silicon Nitride Windows for Correlative Structural and Electrochemical Analysis of Nanomaterials Authors: ['Sasha E. Alden' 'Oluwasegun J. Wahab' 'Lingjie Zhang' 'Kelly L. Vernon' 'Baixu Zhu' 'Kathleen O. Bailey' 'Xingchen Ye' 'Lane A. Baker'] We report an optically transparent carbon electrode-silicon nitride…
Jeanpun Antarasen, Benjamin Wellnitz, Stephanie N. Kramer, Surajit Chatterjee + 1 more
Correlation signal processing of optical three-dimensional (x, y, t) data can produce super-resolution images. The second order cross-correlation function XC_2_ has been documented to produce super-resolution imaging with static and blinking emitters but not for diffusing emitters. Here, we both analytically and…
Hannah Britt, Rebecca Beveridge, Antonio Calabrese, Hannah Ochner + 2 more
Inline low-energy electron holography (LEEH) in conjunction with sample preparation by electrospray ion beam deposition (ES-IBD) has recently emerged as a promising method for the sub-nanometre-scale single-molecule imaging of biomolecules. The single-molecule nature of the LEEH measurement allows for the mapping of…
Steffi Y. Woo, Luiz H. G. Tizei
heterostructures with electron spectroscopies Authors: ['Steffi Y. Woo' 'Luiz H. G. Tizei'] Abstract: The outstanding properties of transition metal dichalcogenide (TMD) monolayers and their van der Waals (vdW) heterostructures, arising from their structure and the modified electron-hole Coulomb interaction in 2D, make…
Tianbi Zhang, T. Ben Britton
To engineer the next generation of advanced materials we must understand their microstructure, and this requires microstructural characterization. This can be achieved through the collection of high contrast, data rich, and insightful microstructural maps. Electron backscatter diffraction (EBSD) has emerged as a…
O. S. Sokolova, T. S. Trifonova, N. I. Derkacheva, A. V. Moiseenko
Analytical electron microscopy techniques, including energy-dispersive X-ray spectroscopy (EDX) and electron energy-loss spectroscopy (EELS), are employed in materials science and biology to visualize and chemically map diverse elements. This review presents cases of successful identification of nucleic acids in cells…
Authors not listed
Electron diffraction (MicroED/3DED) facilitates atomic structure determination from nano- or microcrystals, allowing structural analysis of dry crystalline powders or mixtures. However, radiation damage and instrument vacuum limit the interrogation of molecular crystals in solution at room temperature. We now present a…
Aniruddha Acharya
This comprehensive review discusses the development of scanning electron microscopy and the application of this technology in different fields such as biology, nanobiotechnology and biomedical science. Besides being a tool for high resolution imaging of surface or topography, the technology is coupled with analytical…
Niko Vlahakis, Arden Clauss, Jose Rodriguez
High-energy electrons induce sample damage and motion at the nanoscale to fundamentally limit determination of molecular structures by electron diffraction. Using fast event-based electron counting (EBEC) detectors, we characterize electron beam-induced crystal lattice reorientations (BIR) that appear to broadly affect…