27 papers · ranked by Valyu relevance
Leonid A. Bendersky, Frank W. Gayle
Electron diffraction via the transmission electron microscope is a powerful method for characterizing the structure of materials, including perfect crystals and defect structures. The advantages of electron diffraction over other methods, e.g., x-ray or neutron, arise from the extremely short wavelength (≈2 pm), the…
J. M. Zuo, J. L. Rouviére
Precession electron diffraction has solved a long-standing challenge in electron diffraction. Further progress promises a general technique for structure determination of difficult crystals.
R. Aiswarya, Jobin Jose, Nenad Simonović, Bratislav P. Marinković + 1 more
R. Aiswarya, 1 Jobin Jose,1, ∗ Nenad Simonovi´c, 2 Bratislav P. Marinkovi´c,2, † and Himadri S. Chakraborty3, ‡ 1 Department of Physics, Indian Institute of Technology Patna, Bihar 801103, India 2 Institute of Physics Belgrade, Pregrevica 118, Belgrade, Serbia 3 School of Natural Sciences, D.L. Hubbard Center for…
Knut Müller, Florian F. Krause, Armand Béché, Marco Schowalter + 6 more
'Vincent Galioit' 'Stefan Löffler' 'Johan Verbeeck' 'Josef Zweck' 'Peter Schattschneider' 'Andreas Rosenauer'] By focusing electrons on probes with a diameter of 50 pm, aberration-corrected scanning transmission electron microscopy (STEM) is currently crossing the border to probing subatomic details. A major challenge…
Niko Vlahakis, Arden Clauss, Jose Rodriguez
High-energy electrons induce sample damage and motion at the nanoscale to fundamentally limit determination of molecular structures by electron diffraction. Using fast event-based electron counting (EBEC) detectors, we characterize electron beam-induced crystal lattice reorientations (BIR) that appear to broadly affect…
Emma V. Beale, David G. Waterman, Corey Hecksel, Jason van Rooyen + 6 more
Micro-Electron Diffraction (MicroED) has recently emerged as a powerful method for the analysis of biological structures at atomic resolution. This technique has been largely limited to protein nanocrystals which grow either as needles or plates measuring only a few hundred nanometres in thickness. Furthermore…
Authors not listed
Electron diffraction (MicroED/3DED) facilitates atomic structure determination from nano- or microcrystals, allowing structural analysis of dry crystalline powders or mixtures. However, radiation damage and instrument vacuum limit the interrogation of molecular crystals in solution at room temperature. We now present a…
Niko Vlahakis, Songrong Qu, Logan Richards, Lygia Silva deMoraes + 3 more
Electron counting helped realize the resolution revolution in single particle cryoEM and is now accelerating the determination of MicroED/3DED structures. Its advantages are best demonstrated by new direct electron detectors capable of fast (kilohertz) event-based electron counting (EBEC). This strategy minimizes the…
Denis Tikhonov
Here, we present a new approach for obtaining radial distribution functions (RDF) from the electron diffraction data using a regularized weighted sine least-squares spectral analysis (rwsLSSA). It allows for explicitly transferring the measured experimental uncertainties in the reduced molecular scattering function to…
Johan Hattne, Max T. B. Clabbers, Michael W. Martynowycz, Tamir Gonen
The combination of high sensitivity and rapid readout makes it possible for electron-counting detectors to record cryogenic electron microscopy data faster and more accurately without increasing the exposure. This is especially useful for MicroED of macromolecular crystals where the strength of the diffracted signal at…
Thorsten B. Blum, Dominique Housset, Max T.B. Clabbers, Eric van Genderen + 6 more
Electron diffraction allows protein structure determination when only nanosized crystals are available. Nevertheless, multiple elastic (or dynamical) scattering, prominent in electron diffraction, is a concern. Current methods for modeling dynamical scattering by multi-slice or Bloch wave approaches are not suitable…
Jian‐Min Zuo, Renliang Yuan, Yu‐Tsun Shao, Haw-Wen Hsiao + 4 more
'Saran Pidaparthy' 'Yang Hu' 'Qun Yang' 'Jiong Zhang'] Transmission electron diffraction is a powerful and versatile structural probe for the characterization of a broad range of materials, from nanocrystalline thin films to single crystals. With recent developments in fast electron detectors and efficient computer…
Nele L. M. Müller, Sebastian Trippel, Karol Długołęcki, Jochen Küpper
'Jochen Küpper'] A dc electron gun, generating picosecond pulses with up to 8 × 106 electrons per pulse, was developed. Its applicability for future time-resolved-diffraction experiments on state- and conformer-selected laser-aligned or oriented gaseous samples was characterized. The focusing electrodes were arranged…
Hiroshi Okamoto, Tatiana Latychevskaia, Hans‐Werner Fink
We show that radiation damage to unstained biological specimens is not an intractable problem in electron microscopy. When a structural hypothesis of a specimen is available, quantum mechanical principles allow us to verify the hypothesis with a very low electron dose. Realization of such a concept requires precise…
Marcus Gallagher-Jones, Karen C. Bustillo, Colin Ophus, Logan S. Richards + 4 more
Nanocrystallography has transformed our ability to interrogate the atomic structures of proteins, peptides, organic molecules and materials. By probing atomic level details in ordered sub-10 nm regions of nanocrystals, approaches in scanning nanobeam electron diffraction extend the reach of nanocrystallography and…
Н. Д. Денисов, Andrey Orekhov, Johan Verbeeck
method for nanopowder samples in a SEM instrument Authors: ['Н. Д. Денисов' 'Andrey Orekhov' 'Johan Verbeeck'] > Abstract. The appearance of direct electron detectors marked a new era for electron diffraction. Their high sensitivity and low noise opens the possibility to extend electron diffraction from transmission…
Koji Yonekura, Tetsuya Ishikawa, Saori Maki-Yonekura
A new cryo-EM system has been developed and investigated for use in protein electron 3D crystallography. The system provides parallel illumination of a coherent 300 kV electron beam to a sample, filters out energy-loss electrons through the sample with an in-column energy filter, and allows rotational data collection…
Tatiana Latychevskaia
In this paper, we present the theoretical background to electron scattering in an atomic potential and the differences between low- and high-energy electrons interacting with matter. We discuss several interferometric techniques that can be realized with low- and high-energy electrons and which can be applied to the…
Johan Hattne, Dan Shi, Calina Glynn, Chih-Te Zee + 4 more
Micro-crystal electron diffraction (MicroED) is an emerging method in cryo-EM for structure determination using nanocrystals. It has been used to solve structures of a diverse set of biomolecules and materials, in some cases to sub-atomic resolution. However, little is known about the damaging effects of the electron…
Max T.B. Clabbers, Michael W. Martynowycz, Johan Hattne, Tamir Gonen
Microcrystal electron diffraction (MicroED) is a powerful technique utilizing electron cryo-microscopy (cryo-EM) for protein structure determination of crystalline samples too small for X-ray crystallography. Electrons interact with the electrostatic potential of the sample, which means that scattered electrons carry…
Tatiana Latychevskaia, Jan Pieter Abrahams
Multiple, dynamical electron diffraction can be simulated by multislice calculations. However, experimental evidence indicates that these calculations overestimate dynamical diffraction. It is shown that ignoring bulk solvent scattering and inelastic scattering in calculations, results in overestimating dynamical…
Pierre Guichard, Arnaud Dochain, Raphaël Marion, Pauline de Crombrugghe de Picquendaele + 4 more
We report fast atom diffraction through single-layer graphene using hydrogen atoms at kinetic energies from 150 to 1200 eV. High-resolution images reveal overlapping hexagonal patterns from coexisting monocrystalline domains. Time-of-flight tagging confirms negligible energy loss, making the method suitable for…
Authors not listed
3D electron diffraction (3D-ED) techniques can be used for structure determination, circumventing challenges posed to conventional and bulk X-ray diffraction techniques such as sub-micron sized crystals, the strong effects of texture, the presence of defects, and polyphasic samples. Such challenges previously prevented…
Ye‐Jin Kim, Levi D. Palmer, Wonseok Lee, Nicholas J. Heller + 1 more
'Scott K. Cushing'] Electron energy-loss spectroscopy (EELS) can measure similar information to X-ray, UV-Vis, and IR spectroscopies but with atomic resolution and increased scattering cross sections. Recent advances in electron monochromators have expanded EELS capabilities from chemical identification to the realms…
Naoya Shibata, Takehito Seki, Gabriel Sánchez-Santolino, Scott D. Findlay + 4 more
'Scott D. Findlay' 'Yuji Kohno' 'Takao Matsumoto' 'Ryo Ishikawa' 'Yuichi Ikuhara'] In scanning transmission electron microscopy (STEM), single atoms can be imaged by detecting electrons scattered through high angles using post-specimen, annular-type detectors. Recently, it has been shown that the atomic-scale electric…
F. Javier García de Abajo, Albert Polman, Cruz I. Velasco, Mathieu Kociak + 43 more
Over the past century, continuous advancements in electron microscopy have enabled the synthesis, control, and characterization of high-quality free-electron beams. These probes carry an evanescent electromagnetic field that can drive localized excitations and provide high-resolution information on material structures…
Mårten Skogh, Phalgun Lolur, Werner Dobrautz, Christopher Warren + 5 more
There is currently no combination of quantum hardware and algorithms that can provide an advantage over conventional calculations of molecules or materials. However, if or when such a point is reached, new strategies will be needed to verify predictions made using quantum devices. We propose that the electron density…