24 papers · ranked by Valyu relevance
Xinyan Zhao, Tang Yi-Ching, Rivaaj Monsia, Victor Cantu + 6 more
1 McWilliams School of Biomedical Informatics, University of Texas Health Science Center at Houston, 77030, Texas, United States, 2 Department of Computer Science, The University of Texas at Austin, 78712, Texas, United States, 3 Computer Science Department, Worcester Polytechnic Institute, 01609, Massachusetts, United…
Harmon Greenway, Jin Wang
The rapid growth of therapeutic monoclonal antibodies demands greater accessibility to scalable methods of evaluating antigen binding. Homogenous TR-FRET is ideal for preliminary screening but has not been reported to assay these interactions due to their high-affinity and complex solution-phase kinetics. Here we…
Vinícius Zambaldi, David La, Alexander E. Chu, Harshnira Patani + 28 more
'Amy E. Danson' 'Tristan O. C. Kwan' 'Thomas Frerix' 'Rosalia G. Schneider' 'David Saxton' 'Ashok Thillaisundaram' 'Zachary Wu' 'Isabel Moraes' 'O. Lange' 'Eliseo Papa' 'Gabriella Stanton' 'Victor S. Martı́n' 'Sukhdeep Singh' 'Lai Hong Wong' 'Russ Bates' 'Simon Kohl' 'Josh Abramson' 'Andrew Senior' 'Yilmaz Alguel'…
Vishakha Singh, Mandar Bhutkar, Shweta Choudhary, SanketKumar Nehul + 4 more
The optimization of antibodies to attain the desired levels of affinity and specificity holds great promise for development of the next generation therapeutics. This study delves into the refinement and engineering of CDRs through in silico affinity maturation followed by binding validation using ITC and…
Eden Kapcan, Benjamin P. M. Lake, Anthony F. Rullo
of Avidity Driven Therapeutics Authors: Eden Kapcan, Benjamin P. M. Lake, Anthony F. Rullo Avidity driven therapies aim to achieve exceptionally high target binding affinities by carefully integrating a number of weaker affinity binding ligands. This is in contrast to traditional medicinal chemistry strategies that aim…
Nuwan Bandara, Dasun Premathilaka, Sachini Chandanayake, Sahan Hettiarachchi + 4 more
In drug development, the efficacy of an antibody depends on how the antibody interacts with the target antigen. The strength of these interactions gives an indication of how successful an antibody is in neutralizing an antigen. Therefore, the strength, measured by “binding affinity”, is a critical aspect of antibody…
Simon Erlendsson, Kaare Teilum
When characterizing biomolecular interactions, avidity, is an umbrella term used to describe the accumulated strength of multiple specific and unspecific interactions between two or more interaction partners. In contrast to the affinity, which is often sufficient to describe monovalent interactions in solution and…
Sylwia A. Mankowska, Pietro Gatti-Lafranconi, Matthieu Chodorge, Sudharsan Sridharan + 2 more
'Sudharsan Sridharan' 'Ralph R. Minter' 'Florian Hollfelder'] Affinity panning of large libraries is a powerful tool to identify protein binders. However, panning rounds are followed by the tedious re-screening of the clones obtained to evaluate binders precisely. In a first application of Bead Surface Display (BeSD)…
Matthew J. Styles, Joshua A. Pixley, Tongyao Wei, Chris Basile + 2 more
Proteins that selectively bind to a target of interest are foundational components of research pipelines^1,2^, diagnostics^3^, and therapeutics^4^. Current immunization-based^5,6^, display- based^7–14^, and computational approaches^15–17,18^ for discovering binders are laborious and time- consuming – taking months or…
Basile Nguyen, David Hartich, Udo Seifert, Paolo De Los Rios
The 70 kDa Heat Shock Proteins Hsp70 have several essential functions in living systems, such as protecting cells against protein aggregation, assisting protein folding, remodeling protein complexes and driving the translocation into organelles. These functions require high affinity for non-specific amino-acid…
Steffanie Paul, Edward P. Harvey, James Osei-Owusu, Aaron W. Kollasch + 13 more
Antibodies can bind their targets with exquisite potency and selectivity due in part to large antibody-target protein-protein interaction surface areas. Despite the very large size and diversity of synthetic libraries, in vitro sorting alone tends to yield binders with modest affinities. By analogy to the in vivo…
Hongtai Jing, Zhengtao Gao, Sheng Xu, Tao Shen + 6 more
'Shwai He' 'Tao You' 'Shuang Ye' 'Wei Lin' 'Siqi Sun'] In recent decades, antibodies have emerged as indispensable therapeutics for combating diseases, particularly viral infections. However, their development has been hindered by limited structural information and labor-intensive engineering processes. Fortunately…
Lei Wang, Xiaoming He, Gaoxing Guo, Xinzhou Qian + 1 more
Nanobodies have emerged as promising protein therapeutics due to their high-stability, low immunogenicity, and ease of production. However, experimental screening of high-affinity nanobodies for specific antigens and their post optimization remain costly and time-consuming, mainly due to the large number of possible…
Rhys Adams, Thierry Mora, Aleksandra M. Walczak, Justin B. Kinney
Despite the central role that antibodies play in the adaptive immune system and in biotechnology, much remains unknown about the quantitative relationship between an antibody's amino acid sequence and its antigen binding affinity. Here we describe a new experimental approach, called Tite-Seq, that is capable of…
Authors not listed
Multivalent lectin-glycan interactions (MLGIs) are widespread and vital for pathogen infection, cell-cell communication and immune regulation, making them attractive therapeutic targets. Despite significant efforts, research progress in MLGI targeting therapeutics remain limited, due to incomplete understanding of the…
Md. Aktar Hossain, Saima Sultana
In silico analysis is a powerful technique to identify better therapeutic interventions. Molecular docking is widely used to screen ligands through analysing binding affinities for target receptors. In this study we screened ligands for two proteins which are potential drug targets: deoxyuridine triphosphate…
Lin Li, Esther Gupta, John Spaeth, Leslie Shing + 2 more
'Rajmonda S. Caceres'] Therapeutic antibody development has become an increasingly popular approach for drug development. To date, antibody therapeutics are largely developed using large scale experimental screens of antibody libraries containing hundreds of millions of antibody sequences. The high cost and difficulty…
Eiji Kinoshita, Emiko Kinoshita-Kikuta, Tohru Koike, Jacek Wisniewski
'Jacek Wisniewski'] Affinity electrophoresis is an important technique that is widely used to separate and analyze biomolecules in the fields of biology and medicine. Both quantitative and qualitative information can be gained through affinity electrophoresis. Affinity electrophoresis can be applied through a variety…
Rahman Basaran, Darshita Budhadev, Amy Kempf, Inga Nehlmeier + 4 more
Multivalent lectin-glycan interactions (MLGIs) are pivotal for viral infections and immune regulation. Their structural and biophysical data are thus highly valuable, not only for the understanding of basic mechanisms but also for designing potent glycoconjugate therapeutics against target MLGIs. However, such…
Authors not listed
Predicting protein-ligand binding affinity from three-dimensional (3D) structural data is a central task in structure-based drug discovery, yet it remains challenging due to limited data availability, structural complexity, and the sparse nature of 3D molecular representations. In this study, we investigate the…
Sanjeeva Srivastava, Burcu Ayoglu, Elin Birgersson, Anja Mezger + 4 more
'Mats Nilsson' 'Mathias Uhlén' 'Peter Nilsson' 'Jochen M. Schwenk'] Antibody microarrays enable parallelized and miniaturized analysis of clinical samples, and have proven to provide novel insights for the analysis of different proteomes. However, there are concerns that the performance of such direct labeling and…
Oladapo Olaleye, Natalia Govorukhina, Nico C. van de Merbel, Rainer Bischoff + 1 more
'Rainer Bischoff' 'Vladimir N. Uversky'] There is often a need to isolate proteins from body fluids, such as plasma or serum, prior to further analysis with (targeted) mass spectrometry. Although immunoglobulin or antibody-based binders have been successful in this regard, they possess certain disadvantages, which…
Authors not listed
Glycoconjugates are known to interact with carbohydrate-binding proteins involved in adhesion by pathogens, and offer opportunities to design antimicrobial agents. Metal complexes with Eu(III), Ni(II) and Zn(II) were prepared from glycoconjugate ligand 1Gal, which binds to P. aeruginosa’s lectin LecA (Kd 9.6±0.7 μM).…
Authors not listed
Helicobacter pylori adhesin A (HpaA) is a surface-associated protein critical for the adhesion of H. pylori to gastric epithelial cells, which is strongly associated with an increased risk of gastric cancer. Recent elucidation of the 3D structure of HpaA offers novel opportunities for targeted therapeutic strategies to…