24 papers · ranked by Valyu relevance
Rosalyn Casey, Deena Blumenkrantz, Kerry Millington, Damien Montamat-Sicotte + 8 more
'Damien Montamat-Sicotte' 'Onn Min Kon' 'Melissa Wickremasinghe' 'Samuel Bremang' 'Murphy Magtoto' 'Saranya Sridhar' 'David Connell' 'Ajit Lalvani' 'Denise L. Doolan'] Background IFN-γ and IL-2 cytokine-profiles define three functional T-cell subsets which may correlate with pathogen load in chronic intracellular…
Jesper Gantelius, Tarek Bass, Ronald Sjöberg, Peter Nilsson + 1 more
'Helene Andersson-Svahn'] Protein microarrays are useful tools for highly multiplexed determination of presence or levels of clinically relevant biomarkers in human tissues and biofluids. However, such tools have thus far been restricted to laboratory environments. Here, we present a novel 384-plexed easy to use…
Greg A. Kirchenbaum, Graham Pawelec, Paul V. Lehmann, Roberto Marasca
Determining an individual’s humoral immune reactivity to a pathogen, autoantigen, or environmental agent is traditionally accomplished through the assessment of specific antibody levels in blood. However, in many instances, titers of specific antibodies decline over time and thus do not faithfully reveal prior antigen…
Andrea Palermo, Richard Thelen, Laura K. Weber, Tobias Foertsch + 4 more
'Simone Rentschler' 'Verena Hackert' 'Julia Syurik' 'Alexander Nesterov-Mueller'] Peptide microarrays are a fast-developing field enabling the mapping of linear epitopes in the immune response to vaccinations or diseases and high throughput studying of protein-protein interactions. In this respect, a rapid label-free…
Mattia Bonsignori, M. Anthony Moody
The traditional enzyme-linked immunospot (ELISpot) assay is the gold standard for the enumeration of antigen-specific B cells. Since B cell availability from biological samples is often limited, either because of sample size/volume or the need of performing multiple analyses on the same sample, the implementation of…
Charles J Sande, Timothy Chege, Martin N Mutunga, Elijah Gicheru + 3 more
Despite the considerable progress that has been achieved in the development of vaccines, infectious diseases continue to be the major cause of morbidity and mortality in developing countries. The first year of life remains the time of greatest risk with more than half of all deaths caused by infectious diseases in…
Yi Huang, Heng Zhu
Biomarkers are deemed to be potential tools in early diagnosis, therapeutic monitoring, and prognosis evaluation for cancer, with simplicity as well as economic advantages compared with computed tomography and biopsy. However, most of the current cancer biomarkers present insufficient sensitivity as well as…
Chin Hong Ooi, Nam-Trung Nguyen, Gregor Kijanka
Protein arrays are systematically arranged, large collections of annotated proteins on planar surfaces commonly used for the characterisation of protein binding events against a wide range of possible probes. These may include analyses of protein-protein, peptide-protein, enzyme-substrate or antibody-antigen…
Burcu Gumuscu, Amy Elizabeth Herr
Direct measurement of proteins from single cells has been realized at the microscale using microfluidic channels, capillaries, and semi-enclosed microwell arrays. Although powerful, these formats are constrained, with the enclosed geometries proving cumbersome for multistage assays, including electrophoresis followed…
Takeo Shibata, Sumit Shah, Teresa Evans, Hannah Coleman + 9 more
Advances in high-throughput sequencing have revolutionized the manner with which we can study T cell responses. We describe a woman who received a human papillomavirus (HPV) therapeutic vaccine called PepCan, and experienced complete resolution of her cervical high-grade squamous intraepithelial lesion. By performing…
Francesca Volpetti, José L. García-Cordero, Sebastian J. Maerkl
We present a high-throughput microfluidic platform capable of quantitating up to 384 biomarkers in 4 distinct samples by immunoassay. The microfluidic device contains 384 unit cells, which can be individually programmed with pairs of capture and detection antibody. Samples are quantitated in each unit cell by four…
Adva Raz, Hila Gubi, Adam Cohen, Fernando Patolsky
Biomarkers detection have become essential in medical diagnostics and early detection of life-threatening diseases. Modern-day medicine relies heavily on painful and invasive tests, the extraction of large volumes of venous blood being the most common tool of biomarker detection. These tests are time-consuming…
Sara Feola, Jacopo Chiaro, Beatriz Martins, Salvatore Russo + 11 more
Beside the isolation and identification of MHC-I restricted peptides from the surface of cancer cells, one of the challenges is eliciting an effective anti-tumor CD8+ T cell mediated response as part of therapeutic cancer vaccine. Therefore, the establishment of a solid pipeline for the downstream selection of…
Anastas Pashov, Velizar Shivarov, Maya Hadzhieva, Victor Kostov + 8 more
Specific antibody reactivities are routinely used as biomarkers but the use of antibody repertoire profiles is still awaiting recognition. Here we suggest to expedite the adoption of this class of system level biomarkers by rationally designing a peptide array as an efficient probe for an appropriately chosen…
Sajjad Abdollahramezani, Darrell Omo-Lamai, Gerlof P. Bosman, Omid Hemmatyar + 11 more
'Omid Hemmatyar' 'Sahil Dagli' 'Varun Dolia' 'Kai Chang' 'Nicholas A. Güsken' 'Hamish Carr Delgado' 'Geert‐Jan Boons' 'Mark L. Brongersma' 'Fareeha Safir' 'B.T. Khuri-Yakub' 'Parivash Moradifar' 'Jennifer A. Dionne'] Empirical investigation of the quintillion-scale, functionally diverse antibody repertoires that can be…
Earl F. Bloch, Lou Massa, Kilian Dill
Rudolf Kraus in 1897 described the precipitation reaction from his studies of anticholera and anti-typhoid sera (1). Pauling in 1943 discussed the role of noncovalent forces (columbic, hydrogen bonding, hydrophobic, and Van Der Wall's) involved in antibody-antigen reactions that contribute to precipitation (2).…
Seongyun Choi, Jaeho Ji, Eunjeong Han, Ahrum Son + 1 more
The immune system, fundamental to numerous biological research fields, is increasingly investigated at single-cell resolution using high-dimensional flow cytometry and mass cytometry technologies. These approaches require comprehensive immunomarker information and carefully designed antibody panels that address the…
Nuttawut Kongsuwan, Xiao Xiong, Ping Bai, Jia-Bin You + 3 more
'Ching Eng Png' 'Lin Wu' 'Ortwin Hess'] Plasmon-polaritons are among the most promising candidates for next generation optical sensors due to their ability to support extremely confined electromagnetic fields and empower strong coupling of light and matter. Here we propose quantum plasmonic immunoassay sensing as an…
Hinda Najem, Sebastian Pacheco, Jillyn Turunen, Shashwat Tripathi + 11 more
Sequential multiplex methodologies such as Akoya CODEX, Miltenyi MACSima, Rarecyte Orion, and others require modification of the antibodies by conjugation to an oligo or a specific fluorophore which means the use of off-the-shelf reagents is not possible. Modifications of these antibodies are typically performed via…
Stephanie E Wallace, Martin Kartau, Tarun Kakkar, Christopher Davis + 8 more
'Agnieszka M. Szemiel' 'Emmanuel Mailart' 'Kevin Gautier' 'Adrian J. Lapthorn' 'Nikolaj Gadegaard' 'Malcolm Kadodwala' 'Edward Hutchinson' 'Affar S. Karimullah'] - 1. School of Chemistry, University of Glasgow, Joseph Black Building, University Avenue, G12 8QQ, Glasgow, UK - 2. James Watt School of Engineering…
Amit Patel, Rahul Sharma
The human immune system is a complex network of cells, tissues, and organs that work together to protect the body from harmful pathogens. Antibodies, also known as immunoglobulins (Ig), are small proteins that play a vital role in the immune system's defense mechanism. Among the five classes of immunoglobulins…
Andrea J. Radtke, Colin J. Chu, Ziv Yaniv, Yao Li + 13 more
'Rebecca T. Beuschel' 'Hiroshi Ichise' 'Anita Gola' 'Juraj Kabát' 'Bradley Lowekamp' 'Emily Speranza' 'Joshua Croteau' 'Nishant Thakur' 'Danny Jonigk' 'Jeremy L. Davis' 'Jonathan M. Hernandez' 'Ronald N. Germain'] Andrea J. Radtke1,13,, Colin J. Chu2,3,13, Ziv Yaniv4 , Li Yao5 , James Marr6 , Rebecca T. Beuschel1 …
Eleonora Macchia, Cinzia Di Franco, Cecilia Scandurra, Lucia Sarcina + 6 more
'Matteo Piscitelli' 'Michele Catacchio' 'Mariapia Caputo' 'Paolo Bollella' 'Gaetano Scamarcio' 'Luisa Torsi'] Affiliations: 1Dipartimento di Farmacia-Scienze del Farmaco, Università degli Studi di Bari Aldo Moro, 70125 Bari, Italy; 2Centre for Colloid and Surface Science, Dipartimento di Chimica, Università degli Studi…
Manoj M. Varma
Immunoassay formats applicable for clinical or point-of-care diagnostics fall into two broad classes. One which uses labeled secondary antibodies for signal transduction and the other which does not require the use of any labels. Comparison of the limits of detection (LoD) reported by these two sensing approaches over…