20 papers · ranked by Valyu relevance
Iago Pereiro, Anna Fomitcheva Khartchenko, Robert D. Lovchik, Govind V. Kaigala
'Govind V. Kaigala'] Abstract: Surface assays such as ELISA are pervasive in clinics and research and predominantly standardized in microtiter plates (MTP). MTPs provide many advantages but are often detrimental to surface assay efficiency due to inherent mass transport limitations. Microscale flows can overcome these…
Noémi Becza, Lingling Yao, Paul V. Lehmann, Greg A. Kirchenbaum + 1 more
'Qiao Wang'] Background: Measuring frequencies of antigen-specific memory B cells (Bmem), their immunoglobulin (Ig) class and subclass usage, cross-reactivity, and affinity can provide insights into the efficacy of future antibody responses in case of antigen re-encounter. B cell ImmunoSpot® assays can provide such…
Robayet Chowdhury, Alexander T. Taguchi, Laimonas Kelbauskas, Philip Stafford + 5 more
Past studies have shown that incubation of human serum samples on high density peptide arrays followed by measurement of total antibody bound to each peptide sequence allows detection and discrimination of humoral immune responses to a wide variety of infectious disease agents. This is true even though these arrays…
Greg A. Kirchenbaum, Graham Pawelec, Paul V. Lehmann, Roberto Marasca
Determining an individual’s humoral immune reactivity to a pathogen, autoantigen, or environmental agent is traditionally accomplished through the assessment of specific antibody levels in blood. However, in many instances, titers of specific antibodies decline over time and thus do not faithfully reveal prior antigen…
Greg A. Kirchenbaum, Noémi Becza, Lingling Yao, Alexey Y. Karulin + 3 more
Background/Objectives: Despite clonal expansion during a primary immune response, or after subsequent antigen encounters, the frequency of memory B cells (Bmem) specific for an antigen remains low, making their detection difficult. However, unlike serum antibodies, which have a short half-life in vivo and thus require…
Georgia Stylianou, Sharon Cookson, Justin T. Nassif, Greg A. Kirchenbaum + 3 more
Background: Memory B cells (Bmem) facilitate the generation of renewed and rapid antigen-specific antibody responses long after the initial antigen exposure, at a time when circulating serum antibodies may have declined. As the generation and/or recruitment of Bmem is at the core of most vaccination strategies, the…
Arthur Vengesai, Maritha Kasambala, Hamlet Mutandadzi, Tariro L. Mduluza-Jokonya + 3 more
There is an increasing awareness that health care must move from post-symptomatic treatment to pre-symptomatic intervention . A universal system to diagnose disease, characterize infection or evaluate the response to a vaccine would be useful . An ideal system would allow regular monitoring of health status using…
Chin Hong Ooi, Nam-Trung Nguyen, Gregor Kijanka
Protein arrays are systematically arranged, large collections of annotated proteins on planar surfaces commonly used for the characterisation of protein binding events against a wide range of possible probes. These may include analyses of protein-protein, peptide-protein, enzyme-substrate or antibody-antigen…
Jonathan Walter, Zicki Eludin, Andrei P. Drabovich
Serological diagnostics is generally defined as the detection of specific human immunoglobulins developed against viral, bacterial, or parasitic diseases. Serological tests facilitate the detection of past infections, evaluate immune status, and provide prognostic information. Serological assays were traditionally…
Adva Raz, Hila Gubi, Adam Cohen, Fernando Patolsky
Biomarkers detection have become essential in medical diagnostics and early detection of life-threatening diseases. Modern-day medicine relies heavily on painful and invasive tests, the extraction of large volumes of venous blood being the most common tool of biomarker detection. These tests are time-consuming…
Alexander J McGhee, Eric O. McGhee, Jack E. Famiglietti, W. Gregory Sawyer
'W. Gregory Sawyer'] Advanced cell culture techniques such as 3D bio-printing and hydrogel-based cell embedding techniques harbor many new and exciting opportunities to study cells in environments that closely recapitulate in-vivo conditions. Researchers often study these environments using fluorescence microscopy to…
Stephanie E Wallace, Martin Kartau, Tarun Kakkar, Christopher Davis + 8 more
'Agnieszka M. Szemiel' 'Emmanuel Mailart' 'Kevin Gautier' 'Adrian J. Lapthorn' 'Nikolaj Gadegaard' 'Malcolm Kadodwala' 'Edward Hutchinson' 'Affar S. Karimullah'] - 1. School of Chemistry, University of Glasgow, Joseph Black Building, University Avenue, G12 8QQ, Glasgow, UK - 2. James Watt School of Engineering…
Jin Yu, Alexiane C. Decout, Antonio Di Maio, Wengang Chai + 4 more
Glycan microarrays of sequence-defined glycans are the most widely used approach for high-throughput studies of interactions of glycans with glycan binding proteins. Currently nitrocellulose (NC) coated or N-hydroxysuccinimide (NHS) functionalized glass slides are the two most commonly used surfaces for immobilizing…
Sajjad Abdollahramezani, Darrell Omo-Lamai, Gerlof P. Bosman, Omid Hemmatyar + 11 more
'Omid Hemmatyar' 'Sahil Dagli' 'Varun Dolia' 'Kai Chang' 'Nicholas A. Güsken' 'Hamish Carr Delgado' 'Geert‐Jan Boons' 'Mark L. Brongersma' 'Fareeha Safir' 'B.T. Khuri-Yakub' 'Parivash Moradifar' 'Jennifer A. Dionne'] Empirical investigation of the quintillion-scale, functionally diverse antibody repertoires that can be…
Seongyun Choi, Jaeho Ji, Eunjeong Han, Ahrum Son + 1 more
The immune system, fundamental to numerous biological research fields, is increasingly investigated at single-cell resolution using high-dimensional flow cytometry and mass cytometry technologies. These approaches require comprehensive immunomarker information and carefully designed antibody panels that address the…
Hinda Najem, Sebastian Pacheco, Jillyn Turunen, Shashwat Tripathi + 11 more
Sequential multiplex methodologies such as Akoya CODEX, Miltenyi MACSima, Rarecyte Orion, and others require modification of the antibodies by conjugation to an oligo or a specific fluorophore which means the use of off-the-shelf reagents is not possible. Modifications of these antibodies are typically performed via…
Kenji Sugata, Mitsuyoshi Takatori, Omnia Reda, Benjy Jek Yang Tan + 6 more
Human T-cell leukemia virus type 1 (HTLV-1) has the potential to transform primary CD4^+^ T cells in vitro within a short time; however, the majority of infected individuals maintain an asymptomatic and disease-free condition, suggesting the existence of an equilibrium between the proliferation of infected cells and…
Agostino Occhicone, Alberto Sinibaldi, Peter Munzert, Jordan N. Butt + 4 more
This study presents a rigorous comparative analysis of two label-free optical biosensing platforms, Bloch surface wave (BSW) and microring resonator (MRR), for the detection of SARS-CoV-2 antibodies in human serum. To ensure direct comparability, a new BSW readout system was established alongside an existing MRR…
Amit Patel, Rahul Sharma
The human immune system is a complex network of cells, tissues, and organs that work together to protect the body from harmful pathogens. Antibodies, also known as immunoglobulins (Ig), are small proteins that play a vital role in the immune system's defense mechanism. Among the five classes of immunoglobulins…
Eleonora Macchia, Cinzia Di Franco, Cecilia Scandurra, Lucia Sarcina + 6 more
'Matteo Piscitelli' 'Michele Catacchio' 'Mariapia Caputo' 'Paolo Bollella' 'Gaetano Scamarcio' 'Luisa Torsi'] Affiliations: 1Dipartimento di Farmacia-Scienze del Farmaco, Università degli Studi di Bari Aldo Moro, 70125 Bari, Italy; 2Centre for Colloid and Surface Science, Dipartimento di Chimica, Università degli Studi…