25 papers · ranked by Valyu relevance
Hadas Peled-Zehavi, Reut Yemini, Nir Fluman
Insertion in Cells: Principles, Pathways, and Quality Control Authors: Hadas Peled-Zehavi, Reut Yemini, Nir Fluman Integral membrane proteins comprise at least a quarter of every proteome. To fold and function properly, these proteins must insert into the correct membrane with the proper topology and orientation.…
Authors not listed
Lipid composition drives membrane protein sorting, interactions, and function, but the precise mechanistic influence of the membrane on protein free energy landscapes remains largely unresolved. In this study, we probe how lipids reshape the folding landscape of the pH-low insertion peptide (pHLIP) using a combination…
Jiajia Hong, Xuemei Lu, Zhixiong Deng, Shufeng Xiao + 3 more
'Kai Yang' 'Franco Dosio'] Antimicrobial peptides (AMPs), as a key component of the immune defense systems of organisms, are a promising solution to the serious threat of drug-resistant bacteria to public health. As one of the most representative and extensively studied AMPs, melittin has exceptional broad-spectrum…
Jie Hu, Yiben Fu, Zhongcan Ouyang
Helix-membrane interactions are key to membrane deformation and play significant biological roles. However, systematic studies on the mechanisms behind these interactions are limited. This study uses a continuum membrane model to investigate how shallowly inserted helices interact with biological membranes, focusing on…
Tetiana Serdiuk, Johannes Thoma, Daniel J. Müller
Protein Folding Assisted by Insertases and Translocases Using AFM-Based Single-Molecule Force Spectroscopy Authors: Tetiana Serdiuk, Johannes Thoma, Daniel J. Müller In this review we discuss how atomic force microscopy (AFM)-based single-molecule force spectroscopy (SMFS) approaches can be applied to monitor the…
Ganga P. Sharma, Aaron C. Meyer, Suhail Habeeb, Michael Karbach + 1 more
'Gerhard Müller'] Free-energy landscapes for short peptides – specifically for variants of the pH Low Insertion Peptide (pHLIP) – in the heterogeneous environment of a lipid bilayer or cell membrane are constructed, taking into account a set of dominant interactions and the conformational preferences of the peptide…
Adan Marzouq, Lion Morgenstein, Shimon Yudovich, Ayelet Atkins + 2 more
Insertion of hydrophobic nanoparticles into phospholipid bilayers is limited to small particles that can incorporate into the hydrophobic membrane core in between the two lipid leaflets. Incorporation of nanoparticles above this size limit requires development of challenging surface engineering methodologies. In…
Tristan Bereau, William F. Bennett, Jim Pfaendtner, Markus Deserno + 1 more
'Mikko Karttunen'] The anchor of most integral membrane proteins consists of one or several helices spanning the lipid bilayer. The WALP peptide, GWW(LA)n(L)WWA, is a common model helix to study the fundamentals of protein insertion and folding, as well as helix-helix association in the membrane. Its structural…
Alexander Harjung, Alessandro Fracassi, Neal Devaraj
A common method of generating artificial cells is to encapsulate protein expression systems within lipid vesicles. However, to communicate with the external environment, protein translocation across lipid membranes must take place. In living cells, protein transport across membranes is achieved with the aid of complex…
Thomas Williams, James M. Osborne, Kwok Jian Goh, Trevor Lithgow + 1 more
The outer membrane is the interface through which Gram-negative bacteria - a broad classification of organisms including \textit{Escherichia coli} and a number of deadly pathogens - interact with the environment. Two decades of work on the process of outer membrane biogenesis have led to the discovery of the components…
Authors not listed
Recent advances in cell-penetrating-peptide (CPP)-mediated intracellular protein delivery strategies have highlighted the importance of sustained membrane association to improve delivery efficiency. In this study, we report cell-surface-reactive, polyfluoroalkyl-tagged polyarginine peptides as CPP-additives with…
Edo Vreeker, Adina Sauciuc, Fabian Grünewald, Ali Hammoudi + 1 more
Efficient integration of proteins into amphiphilic polymer membranes offers new opportunities in synthetic biology and nanotechnology. Long-term protein reconstitution into artificial membranes remains challenging due to a lack of stabilising protein-membrane interactions found in native lipid bilayers. Here, we…
Ramakrishnan Natesan, Richard W. Tourdot, Ravi Radhakrishnan
The conformational free energy landscape of a system is a fundamental thermodynamic quantity of importance particularly in the study of soft matter and biological systems, in which the entropic contributions play a dominant role. While computational methods to delineate the free energy landscape are routinely used to…
François Sicard, A. Özgür Yazaydın
Technical challenges in molecule sensing and chemical detection have created an increasing demand for transformative materials with high sensitivity and specificity. Biohybrid nanopores have attracted growing interest as they can ideally combine the durability of solid-state nanopores with the precise structure of…
Asli Aybike Dogan, Martin Dufva
There is a high demand in various fields to develop complex cell cultures. Apart from titer plates, Transwell inserts are the most popular device because they are commercially available, easy to use, and versatile. While Transwell inserts are standardized, there are potential gains to customize inserts in terms of the…
Lingling Gong, Mehran Abbaszadeh Amirdehi, Jayesh Sonawane, Nan Jia + 2 more
Microfluidic microbial fuel cells (MFCs) hold great potential to reproduce core functions of bulk MFCs for study and optimization under precise conditions. Unlike most MFC types, those in a microfluidic format typically do not use a membrane to separate anode and cathode compartments, relying instead on the physics of…
Matthias Wilm
Membrane proteins carry out a wide variety of biological functions. The reproduction of specific properties that have evolved over millions of years of biological membranes in a technically controlled environment is of significant interest. Here a method is presented that allows the self-assembly of a macroscopically…
Matthias Weßling
Sterile filters incorporating endotoxin adsorption function emerged recently to pretreat dialysate liquids fed to a hemodialysis filtration process. Their application significantly enhances the survival rate during dialysis treatment as they function as steril filters as well as an endotoxin adsorber. Little is known…
Feng Luo, Rui Xie, Zhuang Liu, Xiao-Jie Ju + 3 more
'Liang-Yin Chu'] Smart gating membranes, inspired by the gating function of ion channels across cell membranes, are artificial membranes composed of non-responsive porous membrane substrates and responsive gates in the membrane pores that are able to dramatically regulate the trans-membrane transport of substances in…
Authors not listed
Biomass from crop residues is an abundant feedstock for biorefining through both chemical or biological processes. After breaking down intact biomass through physical or chemical means, the resulting pretreatment product contains a wide variety of small molecules that can be funneled into engineered microbes that…
Silvia Tea Calzuola, Gwenyth Newman, Thomas Feaugas, Cécile M. Perrault + 5 more
Microfluidic devices with integrated membranes that enable control of mass transport in constrained environments have shown considerable growth over the last decade. Membranes are a key component in several industrial processes such as chemical, pharmaceutical, biotechnological, food, and metallurgy separation…
Pierre Tapie, Alexis Prevost, Lorraine Montel, Léa-Lætitia Pontani + 1 more
'Élie Wandersman'] We present a simple method to produce giant lipid pseudo-vesicles (vesicles with an oily cap on the top), trapped in an agarose gel. The method can be implemented using only a regular micropipette and relies on the formation of a water/oil/water double droplet in liquid agarose. We characterize the…
Dayinta L. Perrier, Afshin Vahid, Vaishnavi Kathavi, Lotte Stam + 6 more
We study the role of a biomimetic actin cortex during the application of electric pulses that induce electroporation or electropermeabilization, using giant unilamellar vesicles (GUVs) as a model system. The actin cortex, a subjacently attached interconnected network of actin filaments, regulates the shape and…
Arman B. Yeszhanov, Ilya V. Korolkov, Saule S. Dosmagambetova, Maxim V. Zdorovets + 3 more
'Maxim V. Zdorovets' 'Olgun Güven' 'Yu-Hsuan Chiao' 'Ranil Wickramasinghe'] Membrane distillation (MD) is a rapidly developing field of research and finds applications in desalination of water, purification from nonvolatile substances, and concentration of various solutions. This review presents data from recent…
Shimon Yudovich, Adan Marzouqe, Joseph Kantorovitsch, Eti Teblum + 4 more
Supported lipid bilayers are a well-developed model system for the study of membranes and their associated proteins, such as membrane channels, enzymes, and receptors. These versatile model membranes can be made from various components, ranging from simple synthetic phospholipids to complex mixtures of constituents…