26 papers · ranked by Valyu relevance
Yong Wang, Xiushuo Zhang, Jing Xu, Xiangyu Sun + 9 more
'Hongsheng Li' 'Yanping Liu' 'Jingjing Tian' 'Xiaorui Hao' 'Xiaofei Kong' 'Zhiwei Wang' 'Jie Yang' 'Yuqing Su'] As a typical microscopic imaging technology, the emergence of the microscope has accelerated the pace of human exploration of the micro world. With the development of science and technology, microscopes have…
Jason R Swedlow
The current revolution in biological microscopy stems from the realisation that advances in optics and computational tools and automation make the modern microscope an instrument that can access all scales relevant to modern biology - from individual molecules all the way to whole tissues and organisms and from single…
Liyana Valiya Peedikakkal, Andrew Furley, Ashley J. Cadby
Existing optical microscopy techniques compromise between resolution, photodamage, speed of acquisition and imaging in to deep samples. This often confines a technique to a certain biological system or process. We present a versatile imaging system which can switch between imaging modalities with sub millisecond…
Taro Ichimura, Taishi Kakizuka, Kazuki Horikawa, Kaoru Seiriki + 5 more
There are many phenomena in biological systems where less than 1% of rare cells trigger entire multicellular systems. In order to scientifically understand the mechanisms of these rare cell-driven systems, simultaneous observation of huge populations of cells is essential. It has been difficult to observe the dynamics…
Rebecca A. Senft, Barbara Diaz-Rohrer, Pina Colarusso, Lucy Swift + 12 more
Technological advancements in biology and microscopy have empowered a transition from bioimaging as an observational method to a quantitative one. However, as biologists are adopting quantitative bioimaging and these experiments become more complex, researchers need additional expertise to carry out this work in a…
S. Weisenburger, Vahid Sandoghdar
Optical microscopy is one of the oldest scientific instruments that is still used in forefront research. Ernst Abbe's nineteenth century formulation of the resolution limit in microscopy let generations of scientists believe that optical studies of individual molecules and resolving sub-wavelength structures were not…
Shane M. Hickey, Ben Ung, Christie Bader, Robert Brooks + 10 more
'Joanna Lazniewska' 'Ian R. D. Johnson' 'Alexandra Sorvina' 'Jessica Logan' 'Carmela Martini' 'Courtney R. Moore' 'Litsa Karageorgos' 'Martin J. Sweetman' 'Douglas A. Brooks' 'Suleyman Allakhverdiev'] Fluorescence microscopy has become a critical tool for researchers to understand biological processes at the cellular…
Diego Morone, Rocco D’Antuono
The integration of computational advances in microscopy has enhanced our ability to visualise immunological events at scales. However, data generated with these techniques is often complex, multi-dimensional, and multi-modal. Data science and artificial intelligence (AI) play a key role in untangling the wealth of…
Authors not listed
Scanning ion conductance microscopy (SICM) offers non-contact, label-free imaging of live cells with nanometer-scale resolution. However, its conventional imaging mode is inherently slow due to repeated vertical scanning, limiting temporal resolution and causing inertial issues. Here, we present Scanning Counter Ion…
Zengbo Wang
Recently, it was discovered that microsphere can generate super-resolution focusing beyond diffraction limit. This has led to the development of an exciting super-resolution imaging technique -microsphere nanoscopy- that features a record resolution of 50 nm under white lights. Different samples have been directly…
Divya Tripathi, Mayurakshi Hardaniya, Suchita Pande, Dipak Maity + 1 more
'Pierre Gouton'] Optical imaging is an excellent non-invasive method for viewing visceral organs. Most importantly, it is safer as compared to ionizing radiation-based methods like X-rays. By making use of the properties of photons, this technique generates high-resolution images of cells, molecules, organs, and…
Adam J. M. Wollman, Richard Nudd, Erik Hedlund, Mark C. Leake
Although not laying claim to being the inventor of the light microscope, Antonj van Leeuwenhoek, (1632 –1723) was arguably the first person to bring this new technological wonder of the age properly to the attention of natural scientists interested in the study of living things (people we might now term 'biologists').…
Doyeon Kim, SangYun Lee, Moosung Lee, JunTaek Oh + 2 more
Live cell imaging provides essential information in the investigation of cell biology and related pathophysiology. Refractive index (RI) can serve as intrinsic optical imaging contrast for 3-D label-free and quantitative live cell imaging, and provide invaluable information to understand various dynamics of cells and…
Egidijus Auksorius
This thesis centres on the development of multidimensional fluorescence imaging tools, with a particular emphasis on fluorescence lifetime imaging (FLIM) microscopy for application to biological research. The key aspects of this thesis are the development and application of tunable supercontinuum excitation sources…
Gabriel G. Moya Muñoz, Oliver Brix, Philipp Klocke, Nicolas D. Wendler + 3 more
Over the past decades, single-molecule spectroscopy and super-resolution microscopy have advanced significantly and by now represent important tools for life science research. Despite rapid progress and ongoing development, there is a growing gap between the state-of-the-art and what is accessible to non-optics…
Wenbo Jiang, Jingchun Wang, Yidi Yang, Yun Bu + 1 more
Conventional optical microscopes are only able to resolve objects down to a size of approximately 200 nm due to optical diffraction limits. The rapid development of nanotechnology has increased the demand for greater imaging resolution, with a need to break through those diffraction limits. Among super-resolution…
Rong Tang, Jiaze Yin, Bethany Weinberg, Haonan Lin + 4 more
By optically sensing mid-infrared absorption through a visible probe beam, mid-infrared photothermal (MIP) microscopy has emerged as a powerful tool for chemical imaging with micromolar sensitivity and submicron spatial resolution. The adoption of spatially multiplexed camera-based widefield detection further enhanced…
Helen Miller, Zhaokun Zhou, Jack W Shepherd, Adam J. M. Wollman + 1 more
'Mark C. Leake'] Single-molecule biophysics has transformed our understanding of biology, but also of the physics of life. More exotic than simple soft matter, biomatter lives far from thermal equilibrium, covering multiple lengths from the nanoscale of single molecules to up several orders of magnitude to higher in…
Kanda Borgognoni, Bradley Guilliams, Rachel Cohen, Christopher Ackerson
A cloneable NanoParticle (cNP) is an inorganic nanoparticle that is synthesized by a protein. The protein determines the elemental composition, size, morphology, and other properties of the nanoparticle. Here, we describe the use of a cloneable Selenium NanoParticle (cSeNP) as a cloneable imaging contrast agent in…
Keiichiro Toda, Miu Tamamitsu, Ryoichi Horisaki, Takuro Ideguchi
An optical microscope enables image-based findings and diagnosis on microscopic targets, which is indispensable in many scientific, industrial and medical settings. Majority of microscope users are accustomed to standard benchtop microscopes, but it fails to give molecular contrast of the specimen which otherwise…
Authors not listed
Super-resolution imaging techniques that overcome the diffraction limit of light have gained wide popularity for visual-izing cellular structures with nanometric resolution. Although fluorogenic spirocyclization-structural rhodamine dyes remain the premier type of small-molecule fluorophore for super-resolution imaging…
Rananjaya Gamage, Bradley Smith
Indocyanine Blue (ICB) is the deep-red, pentamethine analogue of the widely used clinical near-infrared heptamethine cyanine dye, Indocyanine Green (ICG). The two fluorophores have the same number of functional groups and molecular charge and vary only by a single vinylene unit in the polymethine chain which produces a…
Azaam Aziz, Mariana Medina-Sánchez, Jing Claussen, Oliver G. Schmidt
Medical imaging plays an important role in diagnosis and treatment of multiple diseases. It is a field under continuous development which seeks for improved sensitivity and spatiotemporal resolution to allow the dynamic monitoring of diverse biological processes that occur at the micro- and nanoscale. Emerging…
Domingo Olivares Postigo, Federico Gorrini, Valeria Bitonto, Johannes Ackermann + 3 more
Ensembles of negatively charged nitrogen vacancy centers (NV-) in diamond have been proposed for sensing of magnetic fields and paramagnetic agents, and as a source of spin-order for the hyperpolarization of nuclei in magnetic resonance applications. To this end, strongly fluorescent nanodiamonds represent promising…
Authors not listed
Desorption Electrospray Ionization Mass Spectrometry Imaging (DESI-MSI) is a powerful technique for molecular analysis of surfaces; however, its application of single cell studies has not been previously published. In the current work, a commercial DESI setup (DESI XS) coupled to a mass spectrometer was used to analyze…
Authors not listed
1,4-Dihydronicotinamide adenine dinucleotide hydride (NADH) is an essential coenzyme existing in all living organisms. Due to its involvement in different biological process, fluorescence imaging of intracellular NADH levels at different pathological conditions has emerged as an interesting area of research. We report…