16 papers · ranked by Valyu relevance
Cristina Sisu, Paul Muir, Adam Frankish, Ian Fiddes + 8 more
Pseudogenes are ideal markers of genome remodeling. In turn, the mouse is an ideal platform for studying them, particularly with the availability of developmental transcriptional data and the sequencing of 18 strains. Here, we present a comprehensive genome-wide annotation of the pseudogenes in the mouse reference…
Anne Habedank, Birk Urmersbach, Pia Kahnau, Lars Lewejohann
Existing methods for analysis of home cage based preference tests are either time consuming, not suitable for group management, expensive and/or based on proprietary equipment that is not freely available. For this reason, we developed an automated system for group housed mice based on radio frequency identification…
Michael J. Kuiper, Laurence OW Wilson, Shruthi Mangalaganesh, Carol Lee + 2 more
In silico predictions combined with in vitro, in vivo and in situ observations collectively suggest that mouse adaptation of the SARS-CoV-2 virus requires an aromatic substitution in position 501 or position 498 (but not both) of the spike protein’s receptor binding domain. This effect could be enhanced by mutations in…
Marie-Christine Birling, Atsushi Yoshiki, David J Adams, Shinya Ayabe + 64 more
The International Mouse Phenotyping Consortium reports the generation of new mouse mutant strains for over 5,000 genes from targeted embryonic stem cells on the C57BL/6N genetic background. This includes 2,850 null alleles for which no equivalent mutant mouse line exists, 2,987 novel conditional-ready alleles, and…
Bernardo A. Arús, Emily D. Cosco, Joycelyn Yiu, Ilaria Balba + 3 more
Extracting biological information from awake and unrestrained mice is imperative to in vivo basic and pre-clinical research. Accordingly, imaging methods which preclude invasiveness, anesthesia, and/or physical restraint enable more physiologically relevant biological data extraction by eliminating these extrinsic…
Graeme R. Paul, Peter Schwilch, Esther Wehrle, Gisela A. Kuhn + 1 more
Correct fixation of an object is essential for accurate micro computed tomography scanning. In this document, we provide a motivation for, description of, and use cases for a mouse holder appropriate for holding an externally fixated mouse in femur fracture/defect healing experiments. In addition to rigid fixation, the…
Antonio Muruato, Michelle N. Vu, Bryan A. Johnson, Meredith E. Davis-Gardner + 15 more
The emergence of SARS-CoV-2 has resulted in a worldwide pandemic causing significant damage to public health and the economy. Efforts to understand the mechanisms of COVID-19 disease have been hampered by the lack of robust mouse models. To overcome this barrier, we utilized a reverse genetic system to generate a…
Yingjian Li, Xiaoya Huang, Jikai Deng, Xue Tan + 3 more
Animal models are essential for understanding the pathogenesis of SARS-CoV-2 and for developing therapeutic strategies. Replicon delivery particles (RDPs) were a component of trans-complementary systems for SARS-CoV-2, which is a safe and convenient tool in researching SARS-CoV-2 in animal biosafety level-II laboratory…
Joanna Buchthal, Emma J. Chory, Zachary Hill, Christy Dennison + 9 more
CRISPR/Cas9 technology has revolutionized the production of animal models by reducing experimental timelines, slashing costs and streamlining gene editing, leading to a rapid expansion in the number of unique models for the study of human disease and gene function. However, most non-model animals, many of which are…
Rana Abdelnabi, Caroline S. Foo, Suzanne J. F. Kaptein, Robbert Boudewijns + 5 more
Ancestral SARS-CoV-2 lacks the intrinsic ability to bind to the mouse ACE2 receptor and therefore establishment of SARS-CoV-2 mouse models has been limited to the use of mouse-adapted viruses or genetically modified mice. Interestingly, some of the variants of concern, such as the beta B.1.351 variant, show an improved…
Lok-Yin Roy Wong, Jian Zheng, Kevin Wilhelmsen, Kun Li + 13 more
Coronavirus disease 2019 (COVID-19) is especially severe in aged populations^1^. Resolution of the COVID-19 pandemic has been advanced by the recent development of SARS-CoV-2 vaccines, but vaccine efficacy is partly compromised by the recent emergence of SARS-CoV-2 variants with enhanced transmissibility^2^. The…
Christine M. Bradshaw, Teodora Georgieva, Trevor N. Tankersley, Tama Taylor-Doyle + 4 more
Coronaviridae are significant human pathogens, as evidenced by several outbreaks of severe respiratory infections in the past 20 years and culminating with the COVID-19 pandemic. Mouse models of COVID-19 have included transgenic expression of the main SARS coronavirus entry receptor on human cells, human…
Masataka Nakayama, Shigeru Kyuwa
Mouse hepatitis virus (MHV) is a murine coronavirus and one of the most important pathogens in laboratory mice. Although various strains of MHV have been isolated, they are generally excreted in the feces and transmitted oronasally via aerosols and contaminated bedding. In this study, we attempted to determine the…
Anna Fagre, Juliette Lewis, Miles Eckley, Shijun Zhan + 11 more
Coronavirus disease-19 (COVID-19) emerged in November, 2019 in China and rapidly became pandemic. As with other coronaviruses, a preponderance of evidence suggests the virus originated in horseshoe bats (Rhinolophus spp.) and likely underwent a recombination event in an intermediate host prior to entry into human…
James Brett Case, Suzanne M. Scheaffer, Tamarand L. Darling, Traci L. Bricker + 7 more
The continued evolution and emergence of novel SARS-CoV-2 variants has resulted in challenges to vaccine and antibody efficacy. The emergence of each new variant necessitates the need to re-evaluate and refine animal models used for countermeasure testing. Here, we tested a currently circulating SARS-CoV-2 Omicron…
Meiling Li, Tingyan Lu, Hongwei Zhang, Shufang Chen + 4 more
It is already known that computer keyboards and mouses in hospitals are contaminated with different kinds of bacteria. However, the mouse pad has been neglected with regard to both research and regular cleaning and disinfection in hospitals. In our study, we monitored and evaluated the bacteriology degrees of 74…