23 papers · ranked by Valyu relevance
Natalia Orlova, Dmitri Tsyboulski, Farzaneh Najafi, Sam Seid + 26 more
Cortical columns interact through dynamic routing of neuronal activity. To monitor these interactions, we developed the Multiplane Mesoscope which combines three established microscopy technologies: time-division multiplexing, remote focusing, and random-access mesoscopy. The Multiplane Mesoscope allowed us to study…
Rodrigo Itzamná Becerra-Deana, Simon Desrochers, Raphaël Maltais-Tariant, Simon Brais-Brunet + 4 more
Confocal microscopy is the cornerstone of cellular biology and biomedical research due to its non-destructive imaging, compatibility with live cells, sensitivity, optical sectioning, and subcellular resolution. To meet the demand for rapid three-dimensional imaging, we propose a novel approach using a mode-selective…
Le-Mei Wang, Dhruvam Pandey, Wencai Zhang, Kyu Young Han
Significance Fast, high-throughput fluorescence imaging is essential for numerous biomedical applications, particularly in high-resolution volumetric tissue analysis. Aim We aim to develop an imaging strategy that combines the strengths of multiplane microscopy and extended depth-of-field (EDOF) microscopy and to…
Zhongsheng Zhai, Mingmin Liu, Zili Lei, Zhi Xiong + 5 more
Highlights What are the main findings?1. The integration of a microsphere lens improves lateral resolution from 57.0 lp/mm to 101.6 lp/mm (78.25% increase) primarily through photonic nanojet-assisted near-field coupling and effective numerical aperture enhancement. 2. Dynamic phase encoding on a spatial light modulator…
Alexandr Klioutchnikov, Damian J. Wallace, Caleb Berdahl, Adam Sugi + 2 more
Head-mounted multiphoton microscopes enable imaging of activity from neuronal populations spread throughout the cortical layers in freely moving mice but so far have been restricted to recording from one cortical layer at a time. Here, combining two- and three-photon-based excitation delivered through multiple fibers…
Shuqi Zheng, Minoru Koyama, Jerome Mertz
HiLo microscopy synthesizes an optically-sectioned image from two images, one obtained with uniform and another with patterned illumination, such as laser speckle. Speckle-based HiLo has the advantage of being robust to aberrations, but is susceptible to residual speckle noise that is difficult to control. We present a…
Che‐Yung Shen, Jingxi Li, Yuhang Li, Tianyi Gan + 3 more
'Mona Jarrahi' 'Aydogan Özcan'] Electrical and Computer Engineering Department, University of California, Los Angeles, CA, 90095, USA Bioengineering Department, University of California, Los Angeles, CA, 90095, USA California NanoSystems Institute (CNSI), University of California, Los Angeles, CA, 90095, USA Department…
Karl A. Johnson, Daniel Noble, Rosa Machado, Guy M. Hagen
Fluorescence microscopy provides an unparalleled tool for imaging biological samples. However, producing high-quality volumetric images quickly and without excessive complexity remains a challenge. Here, we demonstrate a simple multi-camera structured illumination microscope (SIM) capable of simultaneously imaging…
Alexandr Klioutchnikov, Damian J. Wallace, Caleb Berdahl, Adam Sugi + 2 more
Head-mounted multiphoton microscopes enable imaging of activity from neuronal populations spread throughout the cortical layers in freely moving mice, but so far have been restricted to recording from one cortical layer at a time. Here, combining 2- and 3-photon based excitation delivered through multiple fibers, we…
Shuqi Zheng, Minoru Koyama, Jerome Mertz
Significance HiLo microscopy synthesizes an optically sectioned image from two images, one obtained with uniform and another with patterned illumination, such as laser speckle. Speckle-based HiLo has the advantage of being robust to aberrations but is susceptible to residual speckle noise that is difficult to control.…
Jun Liao, Zhe Wang, Zibang Zhang, Zichao Bian + 7 more
'Aparna Nambiar' 'Yutong Jiang' 'Shaowei Jiang' 'Jingang Zhong' 'Michael A. Choma' 'Guoan Zheng'] We report the development of a multichannel microscopy for whole-slide multiplane, multispectral, and phase imaging. We use trinocular heads to split the beam path into 6 independent channels and employ a camera array for…
Josiah W. Smith, Murat Torlak
In this article, we introduce a novel algorithm for efficient near-field synthetic aperture radar (SAR) imaging for irregular scanning geometries. With the emergence of fifth-generation (5G) millimeterwave (mmWave) devices, near-field SAR imaging is no longer confined to laboratory environments. Recent advances in…
Ze Zheng, Baolei Liu, Jiaqi Song, Zhu + 8 more
Ze Zheng 1 , 2 , Baolei Liu 3 , ∗ Jiaqi Song 1 , 4 , Muchen Zhu 1 , Yao Wang 1 , Menghan Tian 1 , Ying Xiong 1 , Zhaohua Yang 3 , Xiaolan Zhong 1 , David McGloin 5 , and Fan Wang 1 1 School of Physics, Beihang University, Beijing 100191, China 2 State Key Laboratory of Photonics and Communications, Institute for…
Jae-Young Jang, Hoon Yoo, Robert Sitnik
This paper describes a computational 3-D imaging system based on diffraction grating imaging with laser sources of multiple wavelengths. It was proven that a diffraction grating imaging system works well as a 3-D imaging system in our previous studies. The diffraction grating imaging system has advantages such as no…
Kelsey Hatzell, Yanjie Zheng
X-ray Computed Tomography (CT) is a non-invasive, non-destructive approach to imaging materials, material systems and engineered components in two- and three- dimensions. Acquisition of 3D images requires the collection of hundreds or thousands of through-thickness X-ray radiographic images from different angles. Such…
Liheng Bian, Jinli Suo, Guohai Situ, Ziwei Li + 3 more
'Feng Chen' 'Qionghai Dai'] Existing multispectral imagers mostly use available array sensors to separately measure 2D data slices in a 3D spatial-spectral data cube. Thus they suffer from low photon efficiency, limited spectrum range and high cost. To address these issues, we propose to conduct multispectral imaging…
Liheng Bian, Jinli Suo, Guohai Situ, Ziwei Li + 3 more
'Chen Feng' 'Qionghai Dai'] Current multispectral imagers suffer from low photon efficiency and limited spectrum range. These limitations are partially due to the technological limitations from array sensors (CCD or CMOS), and also caused by separative measurement of the entries/slices of a spatialspectral data cube.…
Jason Deglint, Farnoud Kazemzadeh, Daniel Cho, David A. Clausi + 1 more
'Alexander Wong'] The simultaneous capture of imaging data at multiple wavelengths across the electromagnetic spectrum is highly challenging, requiring complex and costly multispectral image devices. In this study, we investigate the feasibility of simultaneous multispectral imaging using conventional image sensors…
Hongyang Dong, Simon D.M. Jacques, Keith T. Butler, Olof Gutowski + 4 more
In this study, we introduce a method designed to eliminate parallax artefacts present in Xray powder diffraction computed tomography data acquired from large samples. These parallax artefacts manifest as artificial peak shifting, broadening and splitting, leading to inaccurate physicochemical information, such as…
Hongyang Dong, Simon D.M. Jacques, Winfried Kockelmann, Stephen W. T. Price + 10 more
Hongyang Dong 3 , Simon D.M. Jacques 1 , Winfried Kockelmann 4 , Stephen W. T. Price 1 , Robert Emberson 5 , Dorota Matras 6,7 , Yaroslav Odarchenko 1 , Vesna Middelkoop 10 , Athanasios Giokaris 1 , Olof Gutowski 8 , Ann-Christin Dippel 8 , Martin v. Zimmermann 8 , Andrew M. Beale 3 , Keith T. Butler 9 , Antonis…
Authors not listed
Scanning emission-based microscopies, such as X-ray fluorescence (XRF) and energy-dispersive X-ray spectroscopy, offer nanometer-scale chemical maps, but suffer from long acquisition times and radiation damage. Lower-flux and shorter dwell time scans mitigate this problem, but the resulting signal loss can only…
Authors not listed
A thorough understanding of drug pharmacokinetics and target interaction within complex biological environments is critical for successful drug discovery. Quantitative matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI; qMSI) presents a promising avenue for such investigations. While…
Wojciech Michno, Patrick Wehrli, Srinivas Koutarapu, Christian Marsching + 9 more
Understanding of Alzheimer’s disease (AD) pathophysiology, requires molecular assessment of how key pathological factors, specifically amyloid β (Aβ) plaques, influence the surrounding microenvironment. Here, neuronal lipids are particularly of interest as these are implicated in pathological- and neurodegenerative…