26 papers · ranked by Valyu relevance
Ian T. Hoffecker, Alan Shaw, Viktoria Sorokina, Ioanna Smyrlaki + 1 more
Viruses and bacteria commonly exhibit spatial repetition of surface molecules that directly interface with the host immune system. However the complex interaction of patterned surfaces with multivalent immune molecules such as immunoglobulins and B-cell receptors is poorly understood, and standard characterization…
Eike-Christian Wamhoff, Larance Ronsard, Jared Feldman, Grant A. Knappe + 13 more
Protein-based virus-like particles (P-VLPs) are commonly used to spatially organize antigens and enhance humoral immunity through multivalent antigen display. However, P-VLPs are thymus-dependent antigens that are themselves immunogenic and can induce B cell responses that may neutralize the platform. Here, we…
Eike-Christian Wamhoff, Larance Ronsard, Jared Feldman, Blake M. Hauser + 8 more
Multivalent antigen display is a well-established design principle to enhance humoral immunity elicited by subunit vaccines. Protein-based virus-like particles (VLPs) are an important vaccine platform that implements this principle but also contain thymus-dependent off-target epitopes, thereby generating neutralizing…
Zhi-Hua Huang, Zhan-Yi Sun, Yue Gao, Pu-Guang Chen + 3 more
'Yong-Xiang Chen' 'Yan-Mei Li'] Synthetic tumor vaccines have been proven to be promising for cancer immunotherapy. However, the limitation of the specificity and efficiency of the synthetic tumor vaccines need further improvements. To overcome these difficulties, additional tumor-associated targets need to be…
Ana R. Humpierre, Yanira Méndez, Ahyoung Kim, Michael Niemeyer + 8 more
The ability to conjugate multiple molecules to a protein is of great interest for pharmaceutical and vaccine development, especially if this can be achieved in a one-pot procedure. Multicomponent reactions are powerful procedures that allow the assembly of complex constructs incorporating at least three molecular…
Pietro Mastroeni, Omar Rossi, Francesca Micoli, Karen M. Ottemann
Salmonella enterica infections are a major cause of morbidity and mortality worldwide, especially in sub-Saharan Africa and in the Asian continent, and are increasingly associated with antimicrobial resistance. Salmonella enterica serovars Typhi and Paratyphi A, B, and C cause enteric fever, while non-typhoidal…
Gautam Sanyal
A potency or potency-indicating assay is a regulatory requirement for the release of every lot of a vaccine. Potency is a critical quality attribute that is also monitored as a stability indicator of a vaccine product. In essence, a potency measurement is a test of the functional integrity of the antigen and is…
Rochel Hecht, Faez Amokrane Nait Mohamed, Daniel Lingwood, Aaron G. Schmidt
Understanding how antigenic distance influences cross-reactive responses can inform vaccine design. Multivalent displays of viral proteins can improve B cell activation due to receptor cross-linking, and mosaic nanoparticles that incorporate variants can lead to cross-reactive B cell responses recognizing conserved…
Jenny E. Hernandez-Davies, Jiin Felgner, Shirin Strohmeier, Egest James Pone + 8 more
'Egest James Pone' 'Aarti Jain' 'Sharon Jan' 'Rie Nakajima' 'Algimantas Jasinskas' 'Erwin Strahsburger' 'Florian Krammer' 'Philip L. Felgner' 'D. Huw Davies'] Combining variant antigens into a multivalent vaccine is a traditional approach used to provide broad coverage against antigenically variable pathogens, such as…
Rochel Hecht, Faez Amokrane Nait Mohamed, Daniel Lingwood, Aaron Schmidt
Understanding how antigenic distance influences cross-reactive responses can inform vaccine design. Multivalent displays of viral proteins can improve B cell activation due to receptor cross-linking, and mosaic nanoparticles that incorporate variants can lead to cross-reactive B cell responses recognizing conserved…
Jieun Jeong, Jae-Seok Kim, Junghyeon Lee, Yu Ri Seo + 3 more
'Kristine M. Kim' 'Vladimir N. Uversky'] Gonorrhea is one of the most common, but still hidden and insidious, sexually transmitted diseases caused by Neisseria gonorrhoeae (gonococci). However, the diagnosis and treatment of gonorrhea are hampered by antigenic variability among gonococci, the lack of acquired immunity…
Guilherme Meira Lima, Zeinab Jame Chenarboo, Mirat Sojitra, Susmita Sarkar + 14 more
Selective detection of disease-associated changes in the cellular glycocalyx is a foundation of modern targeted therapies. Detecting minor changes in the density and identity of glycans on the cell surface is a technological challenge exacerbated by lack of 1:1 correspondence between cellular DNA/RNA and glycan…
Alisa Mikhaylina, Natalia Lekontseva, Albina Khairetdinova, Nelly Ilyina + 1 more
The study of natural and the design of artificial multivalent proteins is a promising field of molecular biology. Working with such proteins is much more difficult than with their monovalent analogues. In this paper, we show how using a ring of heptameric Sm-like protein as a scaffold, it is possible to create a…
Tal Einav, Shahrzad Yazdi, Aaron T. Coey, Pamela J. Björkman + 1 more
'Rob Phillips'] Due to the low density of envelope (Env) spikes on the surface of HIV-1, neutralizing IgG antibodies rarely bind bivalently using both antigen-binding arms (Fabs) to crosslink between spikes (inter-spike crosslinking), instead resorting to weaker monovalent binding that is more sensitive to Env…
Hsuan-Yuan (Sherry) Wang, Leike Li, Cody S. Nelson, Richard Barfield + 8 more
Human cytomegalovirus (HCMV) remains the most common congenital infection and infectious complication in immunocompromised patients. The most successful HCMV vaccine to-date, an HCMV glycoprotein B (gB) subunit vaccine adjuvanted with MF59, achieved 50% efficacy against primary HCMV infection. A previous study…
Vid Ravnik, Urban Bren, Tine Curk
Selective targeting of membranes with a specific receptor profile is an ongoing challenge in targeted drug delivery. We investigate the adsorption of copolymers on a multicomponent receptor-covered surface using grand-canonical Monte Carlo simulations and demonstrate that polymers can be designed to target a particular…
Tine Curk, Jure Dobnikar, Daan Frenkel
Multivalent particles have the ability to form multiple bonds to a substrate. Hence, a multivalent interaction can be strong, even if the individual bonds are weak. However, much more interestingly, multivalency greatly increases the sensitivity of the particle-substrate interaction to external conditions, resulting in…
Authors not listed
Multivalent lectin-glycan interactions (MLGIs) are widespread and vital for pathogen infection, cell-cell communication and immune regulation, making them attractive therapeutic targets. Despite significant efforts, research progress in MLGI targeting therapeutics remain limited, due to incomplete understanding of the…
Earl F. Bloch, Lou Massa, Kilian Dill
Rudolf Kraus in 1897 described the precipitation reaction from his studies of anticholera and anti-typhoid sera (1). Pauling in 1943 discussed the role of noncovalent forces (columbic, hydrogen bonding, hydrophobic, and Van Der Wall's) involved in antibody-antigen reactions that contribute to precipitation (2).…
Rahman Basaran, Darshita Budhadev, Yuan Guo, Dejian Zhou
The dendritic cell tetrameric lectin, DC-SIGN, and its closely related endothelial cell lectin, DC-SIGNR, (collectively as DC-SIGN/R) play a key role in the binding and transmission of deadly viruses, including Ebola, HIV, HCV, and SARS-CoV-2. Their virus binding/release processes involve a gradually acidifying…
James Hooper, Yuanyuan Liu, Darshita Budhadev, Dejian Zhou + 1 more
Multivalent lectin-glycan interactions (MLGIs) are widely employed for bio- recognition and discrimination, but they are also exploited by pathogens to infect host cells. Their biophysical details (e.g. thermodynamics, kinetics, binding modes and binding site orientation) are thus highly valuable, not only for…
Stefano Angioletti‐Uberti
Nanoparticles functionalized with multiple ligands can be programmed to bind biological targets depending on the receptors they express, providing a general mechanism exploited in various technologies, from selective drug-delivery to biosensing. For binding to be highly selective, ligands should exclusively interact…
Aakansha Nangarlia, Farah Fazloon Hassen, Gabriela Canziani, Praneeta Bandi + 10 more
Host cell infection by SARS-CoV-2, similar to that by HIV-1, is driven by a conformationally metastable and highly glycosylated surface entry protein complex, and infection by these viruses has been shown to be inhibited by the mannose-specific lectins Cyanovirin-N (CV-N) and Griffithsin (GRFT). We discovered in this…
Ana Díaz-Fernández, Simona Ranallo, Francesco Ricci
Here we report the development of a method for the electrochemical ultrasensitive detection of antibodies that couples the programmability and versatility of DNA-based systems with the sensitivity provided by enzymatic amplification. The platform, termed Enzyme-Linked DNA Displacement (ELIDIS), is based on the use of…
Viktoria Dotz, Manfred Wuhrer
Major conclusions: Glycan-based histo-blood groups are associated with intestinal microbiota composition, the risk of various diseases as well as therapeutic success of, e.g., vaccination. Their potential as prebiotic or anti-microbial agents, as disease biomarkers and vaccine targets should be further investigated in…
Brad Evans, Lai Yeung, Armen Beck, Annie Li + 3 more
The growth of therapeutic monoclonal antibodies continues to accelerate due to their success as treatments for many diseases. As new therapeutics are developed, it is increasingly important to have robust bioanalytical methods to measure the pharmacokinetics of the circulating therapeutic monoclonal antibodies (mAbs)…