18 papers · ranked by Valyu relevance
Isabell Lang, Olena Zaitseva, Amelie Glöckler, Daniela Siegmund + 13 more
The receptors of the tumor necrosis factor (TNF) receptor superfamily (TNFRSF) are of overwhelming scientific and clinical relevance and stand at the center of intensive basic and translational research efforts. TNFRSF receptors (TNFRs) are engaged by membrane-bound ligands of the TNF superfamily (TNFSF) and, in some…
Hyeokjune Choi, Hyoin Park, Kabi Son, Ho Min Kim + 1 more
A new two peptide-linked protein assembly strategy was developed for diverse linker length and rigidity variation on protein oligomers.
Nienke Hesen, Mohamed Anany, Andre Freidel, Mediya Baker + 4 more
'Daniela Siegmund' 'Olena Zaitseva' 'Harald Wajant' 'Isabell Lang'] Title: ABSTRACT Most anti-CD40 antibodies show robust agonism only upon binding to FcγR+ cells, such as B cells, macrophages, or DCs, but a few anti-CD40 antibodies display also strong intrinsic agonism dependent on the recognized epitope and/or…
Ivan Vulovic, Qing Yao, Young-Jun Park, Alexis Courbet + 15 more
Protein nanomaterial design is an emerging discipline with applications in medicine and beyond. A longstanding design approach uses genetic fusion to join protein homo-oligomer subunits via α-helical linkers to form more complex symmetric assemblies, but this method is hampered by linker flexibility and a dearth of…
Yang Hsia, Rubul Mout, William Sheffler, Natasha I. Edman + 16 more
A goal of de novo protein design is to develop a systematic and robust approach to generating complex nanomaterials from stable building blocks. Due to their structural regularity and simplicity, a wide range of monomeric repeat proteins and oligomeric helical bundle structures have been designed and characterized.…
Rainer Haag, Rainer Haag
Multivalency is a key principle in nature to establish strong, but also reversible chemical interactions between two units, e.g., a receptor and a ligand, viruses and host cells, or between two cell surfaces. Multivalent binding is based on multiple simultaneous molecular recognition processes and plays an important…
Lihao Ge, Wonchul Shin, Ling-Gang Wu
Vesicle fusion is assumed to occur at flat membrane of excitable cells. In live neuroendocrine cells, we visualized vesicle fusion at Ω-shape membrane generated by preceding fusion, termed sequential compound fusion, which may be followed by fusion pore closure, termed compound kiss-and-run. These novel fusion modes…
Sergey A. Akimov, Oleg V. Kondrashov, Joshua Zimmerberg, Oleg V. Batishchev
'Oleg V. Batishchev'] Enveloped viruses include the most dangerous human and animal pathogens, in particular coronavirus, influenza virus, and human immunodeficiency virus (HIV). For these viruses, receptor binding and entry are accomplished by a single viral envelope protein (termed the fusion protein), the structural…
Rebecca E.A. Gwyther, D. Dafydd Jones, Harley L. Worthy
Protein oligomers are more common in nature than monomers, with dimers being the most prevalent final structural state observed in known structures. From a biological perspective, this makes sense as it conserves vital molecular resources that may be wasted simply by generating larger single polypeptide units, and…
Authors not listed
Membrane fusion underpins many fundamental cellular processes, yet the design of artificial fusogens that can interact with specific cell surface markers in a precise and predictable manner remains a major challenge. Here we demonstrate that cholera toxin B-subunit (CTB), a naturally occurring glycolipid-binding…
Teruyuki Nagamune
Biomolecular engineering can be used to purposefully manipulate biomolecules, such as peptides, proteins, nucleic acids and lipids, within the framework of the relations among their structures, functions and properties, as well as their applicability to such areas as developing novel biomaterials, biosensing…
A Lappala-Vernon, W Nishima, J C Miner, P W Fenimore + 5 more
Membrane fusion proteins are responsible for viral entry into host cells– a crucial first step in viral infection. These proteins undergo large conformational changes from pre-fusion to fusion initiation structures, and, despite differences in viral genomes and disease etiology, many fusion proteins are arranged as…
Ary Lautaro Di Bartolo, Claudia Nora Tomes, Luis Segundo Mayorga, Diego Masone
The fusion pore controls the release of exocytotic vesicles contents through a precise orchestration of lipids from the fusing membranes and proteins. There is a major lipid reorganization during the different stages in life of the fusion pore: membrane fusion, nucleation and expansion, that can be scrutinized…
Ryan E. Odstrcil, Albina O. Makio, McKenna A. Hull, Prashanta Dutta + 2 more
Enveloped viruses must enter host cells to initiate infections through a fusion process, during which the fusion proteins undergo significant and complex structural changes from pre-fusion to post-fusion conformations. Understanding of the fusion protein conformational stability, rapid and accurate identification of…
Ahanjit Bhattacharya, Nahal Bagheri, Steven G. Boxer
Fusion of enveloped viruses with endosomal membranes and subsequent release of viral genome into the cytoplasm is crucial to the viral infection cycle. It is often modelled by carrying out fusion between virus particles and target lipid vesicles. We utilized fluorescence microscopy to characterize the kinetic and…
julio coll
The respiratory syncytial virus (RSV) causes abundant annual fatalities on young children and elder adults by infecting human cells mediated by RSV fusion (F) surface homotrimeric proteins. Despite their RSV fusion in vitro inhibitors, anti-F therapeutic molecules are not yet clinically available because of emergent…
Brian J. Haas, Alexander Dobin, Mahmoud Ghandi, Anne Van Arsdale + 5 more
Gene fusions play a key role as driving oncogenes in tumors, and their reliable discovery and detection is important for cancer research, diagnostics, prognostics and guiding personalized therapy. While discovering gene fusions from genome sequencing can be laborious and costly, the resulting “fusion transcripts” can…
Marcelo Caparotta, Marcelo Puiatti, Diego Masone
Styrene-maleic acid copolymers have become an advantageous detergent-free alternative for membrane protein isolation. Since their discovery, experimental membrane protein extraction and purification by keeping intact their lipid environment has become significantly easier. With the aim of identifying new applications…