17 papers · ranked by Valyu relevance
Alexandra-Madelaine Tichy, Elliot J. Gerrard, Julien M.D. Legrand, Robin M. Hobbs + 1 more
Optogenetics enables the spatio-temporally precise control of cell and animal behaviour. Many optogenetic tools are driven by light-controlled protein-protein-interactions (PPIs) that are repurposed from natural light-sensitive domains (LSDs). Applying light-controlled PPI to new target proteins is challenging because…
Inka Busack, Florian Jordan, Peleg Sapir, Henrik Bringmann
Optogenetics controls neural activity and behavior in living organisms through genetically targetable actuators and light. This method has revolutionized biology and medicine as it allows controlling cells with high temporal and spatial precision. Optogenetics is typically applied only at short time scales, for…
Kirti Sharma, Zoe Jaeckel, Artur Schneider, Oliver Paul + 2 more
Optogenetics involves delivery of light-sensitive opsins to the target brain region, as well as introduction of optical and electrical devices to manipulate and record neural activity, respectively, from the targeted neural population. Combining these functionalities in a single implantable device is of great…
Tayfun Tumkaya, James Stewart, Safwan B. Burhanudin, Adam Claridge-Chang
Optogenetics has become an important tool for the study of behavior, enabling neuroscientists to infer causations by examining behavior after activating genetically circumscribed neurons with light. Light-induced neural activity is affected by illumination parameters used in experiments, such as intensity, duration…
Álvaro Inglés-Prieto, Nikolas Furthmann, Samuel Crossman, Nina Hoyer + 9 more
Optogenetics has been harnessed to shed new mechanistic light on current therapies and to develop future treatment strategies. This has been to date achieved by the correction of electrical signals in neuronal cells and neural circuits that are affected by disease. In contrast, the optogenetic delivery of trophic…
Gregory Gauvain, Himanshu Akolkar, Antoine Chaffiol, Fabrice Arcizet + 20 more
Restoring vision using optogenetics is an ideal medical application because the eye offers a direct window to access and stimulate the pathological area: the retina. Optogenetic therapy could be applied to diseases with photoreceptor degeneration such as retinitis pigmentosa. Here, we select the specific optogenetic…
Karl P. Gerhardt, Evan J. Olson, Sebastian M. Castillo-Hair, Lucas A. Hartsough + 8 more
In optogenetics, researchers use light and genetically encoded photoreceptors to control biological processes with unmatched precision. However, outside of neuroscience, the impact of optogenetics has been limited by a lack of user-friendly, flexible, accessible hardware. Here, we engineer the Light Plate Apparatus…
E. Ronzitti, R. Conti, E. Papagiakoumou, D. Tanese + 6 more
Optogenetic neuronal network manipulation promises to at last unravel a long-standing mystery in neuroscience: how does microcircuit activity causally relate to behavioral and pathological states? The challenge to evoke spikes with high spatial and temporal complexity necessitates further joint development of…
Niall McAlinden, Christopher F. Reiche, Andrew M. Clark, Robert Scharf + 7 more
Optogenetics allows manipulation of neural circuits in vivo with high spatial and temporal precision. However, combining this precision with control over a significant portion of the brain is technologically challenging (especially in larger animal models). Here, we have developed, optimised, and tested in vivo, the…
Lizhu Li, Lihui Lu, Yuqi Ren, Guo Tang + 12 more
The precise control of neural activities at both cellular and circuit levels reveals significant impacts on the fundamental neuroscience explorations and medical applications. Optogenetic methods provide efficient cell-specific modulations, but state-of-the-art technologies lack the ability of simultaneous neural…
Devon J. Griggs, Noah Stanis, Julien Bloch, Jasmine Zhou + 6 more
Optogenetics has been a powerful scientific tool for two decades, yet its integration with non-human primate (NHP) electrophysiology has been limited due to several technical challenges. These include a lack of electrode arrays capable of supporting large-scale and long-term optical access, inaccessible viral vector…
Hidenori Watanabe, Hiromi Sano, Satomi Chiken, Kenta Kobayashi + 4 more
Optogenetics has become an indispensable tool for investigating brain functions. Although non-human primates are particularly useful models for understanding the functions and dysfunctions of the human brain, application of optogenetics to non-human primates is still limited. In the present study, we generated an…
Andrew M. Clark, Alexander Ingold, Christopher F. Reiche, Donald Cundy + 10 more
Optogenetics has transformed studies of neural circuit function, but remains challenging to apply in non-human primates (NHPs). A major challenge is delivering intense and spatially precise patterned photostimulation across large volumes in deep tissue. Here, we have developed and validated the Utah Optrode Array (UOA)…
Niall McAlinden, Yunzhou Cheng, Robert Scharf, Enyuan Xie + 8 more
We present an electrically addressable optrode array capable of delivering light to 181 sites in the brain, each providing sufficient light to optogenetically excite hundreds of neurons in vivo, developed with the aim to allow behavioural studies in large mammals. The device is a glass microneedle array directly…
Simon Weiler, Mateo Vélez-Fort, Troy W. Margrie
Genetic engineering of exogenous opsins sensitive to a wide range of lightwavelengths allows the interrogation of brain circuits to an unprecedented temporal and spatial precision. In particular, red-shifted opsins offer access deeper within the brain tissue. It is however crucial to consider the potential unintended…
Rishi Rajalingham, Michael Sorenson, Reza Azadi, Simon Bohn + 2 more
Challenges in behavioral optogenetics in large brains demand development of a chronically implantable platform for light delivery. We have developed Opto-Array, a chronically implantable array of LEDs for high-throughput optogenetic perturbation in non-human primates. We tested the Opto-Array in the primary visual…
Eliza Bliss-Moreau, Vincent D. Costa, Mark G. Baxter
A recent paper published in Neuron by 15 introduces an openly available resource detailing published and unpublished studies using optogenetics to manipulate the nonhuman primate (NHP) brain. The open science efforts of the team are important and rare in NHP neuroscience, but the conclusions drawn about the success…