26 papers · ranked by Valyu relevance
Peng Tan, Lian He, Yun Huang, Yubin Zhou
To push optogenetics forward toward real-world applications in living animals and ultimately in humans, notable bottlenecks have to be tackled, which include 1) the efficient delivery of the genetically encoded materials to targeted regions, 2) the limited depth of tissue penetration associated with blue and green…
Gagan Acharya, Sebastian F. Ruf, Erfan Nozari
Neurostimulation technologies have seen a recent surge in interest from the neuroscience and controls communities alike due to their proven potential to treat conditions such as epilepsy, Parkinson's Disease, and depression. The provided stimulation can be of different types, such as electric, magnetic, and…
Yuwen Sun, Manrui Li, Shuqiang Cao, Yang Xu + 14 more
'Qian Pan' 'Yadong Guo' 'Yi Ye' 'Zheng Wang' 'Hao Dai' 'Xiaoqi Xie' 'Xiameng Chen' 'Weibo Liang' 'Mootaz Salman' 'Philip Kitchen' 'Roslyn Bill' 'Firas Kobeissy'] Optogenetics is emerging as an ideal method for controlling cellular activity. It overcomes some notable shortcomings of conventional methods in the…
Lauren N. Beloate, Nanyin Zhang
Simultaneously manipulating and monitoring both microscopic and macroscopic brain activity in vivo and identifying the linkage to behavior are powerful tools in neuroscience research. These capabilities have been realized with the recent technical advances of optogenetics and its combination with fMRI, here termed…
Danqing Zhu, Hunter J. Johnson, Jun Chen, David V. Schaffer
Cells reside in a dynamic microenvironment that presents them with regulatory signals that vary in time, space, and amplitude. The cell, in turn, interprets these signals and accordingly initiates downstream processes including cell proliferation, differentiation, migration, and self-organization. Conventional…
Shaowei Li, Jianying Zhang, Jiehui Li, Yajie Hu + 2 more
'Haijun Wang'] Optogenetics and chemogenetics are emerging neuromodulation techniques that have attracted significant attention in recent years. These techniques enable the precise control of specific neuronal types and neural circuits, allowing researchers to investigate the cellular mechanisms underlying depression.…
Andrew M. Clark, Alexander Ingold, Christopher F. Reiche, Donald Cundy + 10 more
Optogenetics has transformed studies of neural circuit function, but remains challenging to apply in non-human primates (NHPs). A major challenge is delivering intense and spatially precise patterned photostimulation across large volumes in deep tissue. Here, we have developed and validated the Utah Optrode Array (UOA)…
Riichiro Hira, Yoshikazu Isomura
We thank Dr. K. Isobe at RIKEN for discussion. This work was supported by JP22wm0525007 (RH), JP19dm0207089 (YI) from AMED, JP22H02731 (RH), JP20K22678 (RH), JP21B304 (RH), JP21H05134 (RH), JP21H05135 (RH), JP21H0524 2(YI), and JP23H02589 (YI) from MEXT/JSPS, JPMJCR1751 (YI) from JST, Nakatani Foundation (RH), Shimadzu…
Tianyu Terry Gao, Teak‐Jung Oh, Kritika Mehta, Yu‐En Andrew Huang + 5 more
1## BACKGROUND Insights into pathogenesis are crucial for disease prevention, diagnosis, management, and treatment. Disease etiology and progression often involve disruptive gene modification, defective intracellular and intercellular cell signaling, and dysregulated cell-environment communications, which could…
Zahra-Soheila Soheili
From the 1970s through the 2000s, microbial opsins were identified as a family of proteins which react to light and shuttle ions through plasma membranes. Single-celled microbes use opsins for movement toward sources of light. By optical control in the milli-second range and accuracy at the level of cell type, the…
Ruben Schoeters, Thomas Tarnaud, Laila Weyn, Wout Joseph + 2 more
Optogenetics has emerged as a promising technique for modulating neuronal activity and holds potential for the treatment of neurological disorders such as temporal lobe epilepsy (TLE). However, clinical translation still faces many challenges. This in-silico study aims to enhance the understanding of optogenetic…
Niall McAlinden, Christopher F. Reiche, Andrew M. Clark, Robert Scharf + 7 more
Optogenetics allows manipulation of neural circuits in vivo with high spatial and temporal precision. However, combining this precision with control over a significant portion of the brain is technologically challenging (especially in larger animal models). Here, we have developed, optimised, and tested in vivo, the…
Devon J. Griggs, Noah Stanis, Julien Bloch, Jasmine Zhou + 6 more
Optogenetics has been a powerful scientific tool for two decades, yet its integration with non-human primate (NHP) electrophysiology has been limited due to several technical challenges. These include a lack of electrode arrays capable of supporting large-scale and long-term optical access, inaccessible viral vector…
Giorgio Scordo, Kostas Kanellopulos, Surangrat Thongkorn, Samuel Tavares da Silva Maraschin + 7 more
Optogenetics has emerged as a powerful tool for disease modeling, enabling precise control of cellular activities through light stimulation and providing a valuable insights into disease mechanisms and therapeutic possibilities. Innovative materials and technologies such as micro-LEDs, optical fibers and micro/nano…
Alexander W. Levis, Gabriel Loewinger, Francisco Pereira
Optogenetics is widely used to study the effects of neural circuit manipulation on behavior. However, the paucity of causal inference methodological work on this topic has resulted in analysis conventions that discard information, and constrain the scientific questions that can be posed. To fill this gap, we introduce…
Simon Weiler, Mateo Vélez-Fort, Troy W. Margrie
Genetic engineering of exogenous opsins sensitive to a wide range of lightwavelengths allows the interrogation of brain circuits to an unprecedented temporal and spatial precision. In particular, red-shifted opsins offer access deeper within the brain tissue. It is however crucial to consider the potential unintended…
Sanjana Shetty
Neural networks have been employed for a wide range of processing applications like image processing, motor control, object detection and many others. Living neural networks offer advantages of lower power consumption, faster processing, and biological realism. Optogenetics offers high spatial and temporal control over…
Luke Shaw, Krishnan Padmanabhan, Amy Buckleaw, Jude F. Mitchell + 1 more
The primate cerebral cortex relies on long-range connections to integrate information between functionally specialized areas. Investigating these processes requires tools that can selectively modulate specific projection pathways. While cell-class-specific optogenetics can modulate local circuits, these approaches…
Authors not listed
Channelrhodopsin-2 (ChR2) is a light-gated ion channel widely used in optogenetics, a technique that enables precise control of neuronal activity by genetically engineering light-sensitive proteins into cell membranes. This protein exists in dimeric form, with each monomer containing a retinal Schiff base (RSB) moiety…
Nan Zheng, Ying Jiang, Shan Jiang, Jongwoon Kim + 4 more
'Ji‐Xin Cheng' 'Xiaoting Jia' 'Chen Yang'] C.Y. conceived the project. N.Z. and S.J. performed fabrication and characterization of materials. N.Z. and Y.J. performed the stimulation and recording experiments in vitro and in vivo. N.Z. and Y.L. performed the in vivo biocompatibility evaluations. X.J. provided guidance…
Yue‐Ming Li, Ying Jiang, Lu Lan, Xiaowei Ge + 8 more
'Yuewei Zhan' 'Linli Shi' 'Nan Zheng' 'Guo Chen' 'Runyu Wang' 'Yang Chen' 'Ji‐Xin Cheng'] High precision neuromodulation is a powerful tool to decipher neurocircuits and treat neurological diseases. Current non-invasive neuromodulation methods offer limited precision at the millimeter-level. Here, we report…
Spencer Kim, Dylan Gray, Michelle Shanguhyia, Rachel Steinhardt
Recreating the signaling profile a chemical synapse to analyze serotonin receptor activation is a challenge. This is due in part to the kinetics of the synapse, where neurotransmitters are rapidly released and quickly cleared by active reuptake machinery. One strategy to produce a rapid rise in a bio-orthogonally…
Giacomo Mazzotti, Denis Hartmann, Michael Booth
Cell-free expression of a gene to protein has become a vital tool in nanotechnology and synthetic biology. Remote-control of cell-free systems with multiple, orthogonal wavelengths of light would enable precise, non-invasive modulation, opening many new applications in biology and medicine. While there has been success…
Afsaneh Taheri Kal-Koshvandi
We present the first light-free, genetically minimal platform for spatiotemporally resolved proximity labeling across five neuronal compartments—synaptic, nuclear, endosomal, mitochondrial, and activity-dependent microdomains. A covalent "catalyst–luciferase sandwich" architecture site-specifically conjugates five…
Authors not listed
Photocages have emerged as powerful tools for enabling precise control over the timing, location and quantity of the released biomolecule of interest. However, most commonly used photocages require activation by UV or violet light, which is cytotoxic. Moreover, the exclusive control of light on their photoreactivity…
Authors not listed
Spatiotemporal control of molecular release is essential for the precise manipulation of cellular functions, and photo-stimulation is one of the most widely used strategies to achieve this control. However, direct light exposure can cause cell damage due to the generation of reactive oxygen species and photothermal…