24 papers · ranked by Valyu relevance
Exequiel Porta, Karunakaran Kalesh, Jaime Isern, Patrick Steel
Identifying new enzymes and gaining insights into their mode of action (and inhibition) is a critical step in drug discovery. In this chapter, we describe a novel methodology that accelerates this process by combining the precision of Activity-Based Protein Profiling (ABPP) with the efficiency of microplate assay…
Hyeonna Choi, Jung Yup Kim, Hyuneui Lim, Seunggyu Jeon
Biological experiment protocols are written in natural language, whereas automation systems rely on predefined control commands, creating a semantic gap that limits autonomous execution. Microplate-based automatic experiments are particularly challenging due to the need to simultaneously control well mapping…
Jontana Allkja, Frits van Charante, Juliana Aizawa, Inés Reigada + 11 more
'Clara Guarch-Pérez' 'Jesus Augusto Vazquez-Rodriguez' 'Paul Cos' 'Tom Coenye' 'Adyary Fallarero' 'Sebastian A. J. Zaat' 'Antonio Felici' 'Livia Ferrari' 'Nuno F. Azevedo' 'Albert E. Parker' 'Darla M. Goeres'] Microtiter plate methods are commonly used for biofilm assessment. However, results obtained with these…
Authors not listed
This paper details a 3D-printed plate handler designed to address the limitation of automated plate manipulation within the Opentrons OT-2 liquid handling robot. The hardware enables precise, automated labware transfer, expanding the OT-2’s capabilities for complex biological and chemical assays. Technically, it…
Janith Wanigasekara, Lara J. Carroll, Patrick J. Cullen, Brijesh Tiwari + 2 more
'Brijesh Tiwari' 'James F. Curtin' 'Nils Cordes'] Three-dimensional (3D) cell culture models can help bridge the gap between in vitro cell cultures and in vivo responses by more accurately simulating the natural in vivo environment, shape, tissue stiffness, stressors, gradients and cellular response while avoiding the…
William Benman, Pavan Iyengar, Thomas Mumford, Lukasz J. Bugaj
Temperature is a critical parameter for biological function, yet there is a lack of approaches to modulate the temperature of biological specimens in a dynamic and high-throughput manner. We present the thermoPlate, a device for programmable control of temperature in each well of a 96-well plate in a manner compatible…
Victoria Probst, Felix Pacheco, Finn Cilius Nielsen, Frederik Otzen Bagger
Single cell mRNA sequencing technologies have transformed our understanding of cellular heterogeneity and identity. For sensitive discovery or clinical marker estimation where high transcript capture per cell is needed only plate-based techniques currently offer sufficient resolution. Here, we present a performance…
John A. Bryant, Cameron Longmire, Sriya Sridhar, Samuel Janousek + 2 more
Every year biotechnology labs generate a combined total of ∼5.5 million tons of plastic waste. As the global bioeconomy expands, biofoundries will inevitably increase plastic consumption in-step with synthetic biology scaling. Decontamination and reuse of single-use plastics could increase sustainability and reduce…
Vibecke Markhus, Katarina Fritz-Wallace, Olav Mjaavatten, Einar K. Kristoffersen + 2 more
Platelets play a critical role in hemostasis and are involved in a wide range of physiological and pathological processes, including cancer, inflammation, atherosclerosis, and angiogenesis. Their dynamic and adaptable proteome not only supports their essential functions in normal physiological conditions but also…
Authors not listed
Ion transport across biological lipid membranes is crucial for both health and disease, prompting investigation into synthetic ion transporters as potential therapeutics. However, state of the art methods for assessing transporter activity are often time-consuming and resource-intensive, hindering the screening of…
María Andreína Francisco Rodríguez, Jordi Carreras Puigvert, Ola Spjuth
Microplates are indispensable in large-scale biomedical experiments but the physical location of samples and controls on the microplate can significantly affect the resulting data and quality metric values. We introduce a new method based on constraint programming for designing microplate layouts that reduces unwanted…
Tomoyuki Kawase, Katsuya Suzuki, Masami Kamimura, Tomoharu Mochizuki + 3 more
'Takashi Ushiki' 'Fernando Albericio' 'Jennifer Patterson'] Platelet polyphosphate (polyP) can be conveniently quantified by exploiting a recent methodological breakthrough using 4′,6-diamidino-2-phenylindole (DAPI). However, the preservation of these biological samples has not yet been standardized. In a preliminary…
Iago Pereiro, Anna Fomitcheva Khartchenko, Robert D. Lovchik, Govind V. Kaigala
'Govind V. Kaigala'] Abstract: Surface assays such as ELISA are pervasive in clinics and research and predominantly standardized in microtiter plates (MTP). MTPs provide many advantages but are often detrimental to surface assay efficiency due to inherent mass transport limitations. Microscale flows can overcome these…
Authors not listed
Therapeutic drug monitoring enables personalized cancer medicine by dosing patients with chemotherapy based on the patient’s own unique rate of drug clearance and metabolic degradation. These rates significantly influence the plasma concentration which influences patient outcomes. This is especially true for the…
SangYun Lee, Seongsoo Jang, YongKeun Park
Platelets, or thrombocytes, are anucleated tiny blood cells with an indispensable contribution to the hemostatic properties of whole blood, detecting injured sites at the surface of blood vessels and forming blood clots. Here, we quantitatively and non-invasively investigated the morphological and biochemical…
Michaela Oeller, Sandra Laner-Plamberger, Linda Krisch, Eva Rohde + 3 more
Numerous cell-based therapeutics are currently being tested in clinical trials. Human platelet lysate (HPL) is a valuable alternative to fetal bovine serum as a cell culture medium supplement for a variety of different cell types. HPL as a raw material permits animal serum-free cell propagation with highly efficient…
Scott Custo, Byron Baron, Alex Felice, Elisa Seria
Objectives: Platelet-derived products have been shown as promising novel therapeutic agents for chronic wounds. However, their clinical use requires a greater degree of method standardisation, part of which involved more extensive cataloguing of their biochemical composition. This study aimed to quantify and compare…
Tairan Liu, Yuzhu Li, Hatice Ceylan Koydemir, Yijie Zhang + 5 more
'Yi‐Chen E. Yang' 'Hongda Wang' 'Jingxi Li' 'Bijie Bai' 'Aydogan Özcan'] 1 Electrical and Computer Engineering Department, University of California, Los Angeles, CA, 90095, USA. 2 Bioengineering Department, University of California, Los Angeles, 90095, USA. 3 California NanoSystems Institute (CNSI), University of…
Alice Lorusso, Liam Morgan, Aileen Farrelly, Mark Lambert + 6 more
Platelet count, mean platelet volume (MPV) and residual leucocyte content were measured using a Sysmex XN-1000™ haematology analyser (Sysmex Corporation, Japan). Metabolic parameters (pH, pO2, pCO2) were assessed using the Roche Omni S blood gas analyser (Roche Diagnostics, Switzerland). Glucose concentration was…
Dwi Aprilia Mahfirotin, Brian Ferliano, Andrian Dede Handika, Yosi Sudarsi Asril + 7 more
1## INTRODUCTION The American Association of Physicists in Medicine (AAPM) and the International Atomic Energy Agency (IAEA) published protocols for clinical reference dosimetry in external beam radiation therapy using high-energy photon and electron beams., The protocols are AAPM Task Group 51 (TG-51) and IAEA…
Trina De, Vardan Andriasyan, Artur Yakimovich
Virological plaque assays are the primary method for quantifying infectious particles in a suspension, achieved by incubating a serial dilution of the virus with a monolayer of indicator cells. Existing software tools for quantification of plaque assay images lack modularity, show measurements disagreement or are…
Siting Liu, Gong Chen, An Mei, Xunjie Lu + 3 more
Microfluidics has emerged as a rapidly growing field in recent years, offering numerous advantages over traditional methods for various applications such as biomedical analysis, environmental monitoring, and chemical synthesis. Among the many areas of microfluidics research, the manipulation of fluids plays a crucial…
Harriet E. Allan, Melissa A. Hayman, Simone Marcone, Melissa V. Chan + 9 more
Anucleate platelets circulate in the blood of healthy individuals for approximately 7-10 days during which time their protein composition may change. We hypothesized such changes would be linked to altered structure and function. Here, we separated platelets of different ages based on mRNA content and characterised…
Brian Hsu, Priyanka V Setty, Rory Butler, Ryan Lewis + 6 more
DRAFT Automating experimental protocol design and execution remains as a fundamental bottleneck in realizing self-driving laboratories. We introduce PRISM (Protocol Refinement through Intelligent Simulation Modeling), a framework that automates the design, validation, and execution of experimental protocols on a…